Anti-AHNAK antibody [EM-09]

5 product images

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  Immunocytochemistry - Anti-AHNAK antibody [EM-09] (ab68556)

  Human primary fibroblasts stained with ab68556 (green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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  Western blot - Anti-AHNAK antibody [EM-09] (ab68556)

  The material was 1% lauryl maltoside lysate of HeLa cells, non-reducing conditions, immunoprecipitated with another anti-AHNAK1 antibody. 4% stacking gel, 6% separating gel, U=190 V, 60 min. Blotting: 30 mA, 90 min. Detection antibody concentration: 2 microgram/ml. Please note the conditions were not optimalized.

  All lanes: Western blot - Anti-AHNAK antibody [EM-09] (ab68556) at 2 µg/mL

  Performed under non-reducing conditions.

  Predicted band size: 629 kDa

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  Immunocytochemistry - Anti-AHNAK antibody [EM-09] (ab68556)

  ab68556 at 1/250 dilution staining AHNAK (red) in HeLa cells by Immunocytochemistry/Immunofluorescence. Nuclei were stained with DAPI (blue).

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  Flow Cytometry - Anti-AHNAK antibody [EM-09] (ab68556)

  Overlay histogram showing HeLa cells stained with ab68556 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68556, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  

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  Immunohistochemistry (Frozen sections) - Anti-AHNAK antibody [EM-09] (ab68556)

  ab68556 (red) staining AHNAK in murine tongue by immunohistochemistry using frozen tissue sections. Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).