AB68556

Anti-AHNAK antibody [EM-09]

Name: Anti-AHNAK antibody [EM-09] (ab68556) | Abcam
Brand: Abcam Limited
SKU: AB68556
Price: 520 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(6 Publications)

Mouse Monoclonal AHNAK antibody. Suitable for ICC, Flow Cyt, WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AHNAK.

View Alternative Names

PM227, AHNAK, Neuroblast differentiation-associated protein AHNAK, Desmoyokin

5 Images

Flow Cytometry - Anti-AHNAK antibody [EM-09] (AB68556)

Flow Cyt

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Flow Cytometry - Anti-AHNAK antibody [EM-09] (AB68556)

Overlay histogram showing HeLa cells stained with ab68556 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68556, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Immunocytochemistry - Anti-AHNAK antibody [EM-09] (AB68556)

ab68556 at 1/250 dilution staining AHNAK (red) in HeLa cells by Immunocytochemistry/Immunofluorescence. Nuclei were stained with DAPI (blue).

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Immunocytochemistry - Anti-AHNAK antibody [EM-09] (AB68556)

Human primary fibroblasts stained with ab68556 (green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunohistochemistry (Frozen sections) - Anti-AHNAK antibody [EM-09] (AB68556)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-AHNAK antibody [EM-09] (AB68556)

ab68556 (red) staining AHNAK in murine tongue by immunohistochemistry using frozen tissue sections. Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).

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Western blot - Anti-AHNAK antibody [EM-09] (AB68556)

The material was 1% lauryl maltoside lysate of HeLa cells, non-reducing conditions, immunoprecipitated with another anti-AHNAK1 antibody. 4% stacking gel, 6% separating gel, U=190 V, 60 min. Blotting : 30 mA, 90 min. Detection antibody concentration : 2 microgram/ml. Please note the conditions were not optimalized.

All lanes:

Western blot - Anti-AHNAK antibody [EM-09] (ab68556) at 2 µg/mL

Predicted band size: 629 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EM-09

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

ICC, IHC-Fr, Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human AHNAK. The exact immunogen used to generate this antibody is proprietary information.

Q09666

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/250", "ICC-species-notes": "Permeabilization is required.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "1/250", "ICC-species-notes": "Permeabilization is required.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab68556 is purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AHNAK protein also known as "giant ankyrin repeat-containing protein" or "desmoyokin" has a large molecular mass of approximately 700 kDa. It is expressed in various tissues including heart skeletal muscle and brain. The protein is characterized by its repeated ankyrin-like motifs which are essential for protein-protein interactions. AHNAK is localized to the plasma membrane cytoplasm and sometimes the nucleus indicating its versatile roles within cells.

Biological function summary

AHNAK has key functions in cell structure and signaling. It can form a complex with other proteins such as S100 family proteins influencing calcium binding and signal transduction. This complex plays a role in the regulation of the cytoskeleton and membrane repair processes. Additionally AHNAK participates in cellular stress responses and impacts cell adhesion by interacting with membrane-associated proteins.

Pathways

AHNAK interacts with pathways involving calcium signaling and cytoskeletal organization. It links to the calcium-binding protein S100B facilitating downstream signaling that impacts cellular responses to external stimuli. Another pathway involves plasma membrane repair where AHNAK partners with annexin proteins to reseal damaged membranes and maintain cellular integrity.

AHNAK associates with cardiomyopathies and certain types of cancer. In cardiac conditions its interaction with proteins like dystrophin is important for maintaining muscle cell integrity during contraction. The dysregulation of AHNAK in cancer can influence cell motility and invasiveness highlighting its potential as a biomarker for tumor progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be required for neuronal cell differentiation.

See full target information AHNAK

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:7900-7920 PubMed36261270

2022

Activity-Induced Cortical Glutamatergic Neuron Nascent Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Lucio M Schiapparelli,Yi Xie,Pranav Sharma,Daniel B McClatchy,Yuanhui Ma,John R Yates,Anton Maximov,Hollis T Cline

Clinical and experimental immunology 209:291-304 PubMed35766885

2022

The AHNAK induces increased IL-6 production in CD4+ T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss.

Applications

Unspecified application

Species

Unspecified reactive species

Liman Li,Yuan Liu,Ting Feng,Wenjie Zhou,Yanyun Wang,Hong Li

Cancer cell international 20:76 PubMed32190000

2020

MicroRNA-93-5p promotes epithelial-mesenchymal transition in gastric cancer by repressing tumor suppressor AHNAK expression.

Applications

Unspecified application

Species

Unspecified reactive species

Erdong Shen,Xin Wang,Xin Liu,Mingyue Lv,Liang Zhang,Guolian Zhu,Zhe Sun

Journal of Cancer 10:3860-3870 PubMed31333803

2019

Upregulation of nucleoprotein AHNAK is associated with poor outcome of pancreatic ductal adenocarcinoma prognosis via mediating epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwen Zhang,Xiaoding Liu,Rui Huang,Xuguang Liu,Zhiyong Liang,Tonghua Liu

Molecular cancer research : MCR 16:1287-1298 PubMed29724814

2018

AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Won Park,Il Yong Kim,Ji Won Choi,Hee Jung Lim,Jae Hoon Shin,Yo Na Kim,Seo Hyun Lee,Yeri Son,Mira Sohn,Jong Kyu Woo,Joseph H Jeong,Cheolju Lee,Yun Soo Bae,Je Kyung Seong

Biologicals : journal of the International Associa 52:59-66 PubMed29361371

2018

Mass spectrometry analysis reveals differences in the host cell protein species found in pseudotyped lentiviral vectors.

Applications

Species

Unspecified reactive species

Sabine Johnson,Jun X Wheeler,Robin Thorpe,Mary Collins,Yasuhiro Takeuchi,Yuan Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AHNAK antibody [EM-09]

Flow Cytometry - Anti-AHNAK antibody [EM-09] (AB68556)

Immunocytochemistry - Anti-AHNAK antibody [EM-09] (AB68556)

Immunocytochemistry - Anti-AHNAK antibody [EM-09] (AB68556)

Immunohistochemistry (Frozen sections) - Anti-AHNAK antibody [EM-09] (AB68556)

Western blot - Anti-AHNAK antibody [EM-09] (AB68556)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise