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Rabbit Polyclonal AHNAK2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein AHNAK2 aa 2600-2750.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-AHNAK2 antibody (AB224055), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHNAK2 antibody (AB224055), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHNAK2 antibody (AB224055), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Protein AHNAK2 aa 2600-2750. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVF2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Associated Products

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Alternative names

Recommended products

Rabbit Polyclonal AHNAK2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein AHNAK2 aa 2600-2750.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Protein AHNAK2 aa 2600-2750. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVF2
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

AHNAK2 short for "AHNAK nucleoprotein 2" is a large ubiquitously expressed protein with a molecular mass of approximately 600 kDa. Scientists have identified it in various tissues with high levels in the heart lungs and skin. This protein belongs to the family of nucleoproteins which suggests its involvement in cellular structural functions. AHNAK2 is known to play a role in structural integrity as it interacts with components of the cytoskeleton and cell membrane.

Biological function summary

AHNAK2 influences the cellular processes of migration and proliferation. It interacts with members of the actin cytoskeleton and certain signaling pathways. AHNAK2 does not form part of a well-defined complex but shows associations with signaling molecules that manage cell shape and motility. Its interactions indicate a potential regulatory function in rearranging cytoskeletal structures which facilitates various dynamic cellular processes.

Pathways

AHNAK2 participates in the TGF-beta signaling pathway and the MAPK/ERK pathway. It strengthens these pathways especially by modulating cellular responses to external stimuli. The protein also associates with other signaling molecules like SMAD proteins in TGF-beta pathway contributing to the regulation of gene expression cell growth and development. AHNAK2’s involvement in this network indicates its role in turning extracellular information into intracellular actions.

Associated diseases and disorders

Researchers have linked AHNAK2 to forms of cancer especially lung and pancreatic cancer. Elevated levels of AHNAK2 may relate to tumor progression and metastasis. Additional studies associate it with proteins like KRAS involved in signal transduction which suggests AHNAK2 might encourage oncogenic pathways that drive cancer development. Understanding AHNAK2 functions and interactions could lead to new insights into therapeutic strategies.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-AHNAK2 antibody (ab224055), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AHNAK2 antibody (ab224055)

    PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for AHNAK2 (green) using ab224055 (4 μg/ml) in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHNAK2 antibody (ab224055), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHNAK2 antibody (ab224055)

    Paraffin embedded human skin tissue stained for AHNAK2 with ab224055 at a 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHNAK2 antibody (ab224055), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHNAK2 antibody (ab224055)

    Paraffin embedded human skin tissue stained for AHNAK2 with ab224055 at a 1/500 dilution in immunohistochemical analysis. No staining is observed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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