Rabbit Recombinant Monoclonal Aryl hydrocarbon Receptor antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AHR aa 750 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHR antibody [BLR118H] - BSA free (AB314060), expandable thumbnail](https://content.abcam.com/products/images/ahr-antibody-blr118h-bsa-free-ab314060--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420442.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHR antibody [BLR118H] - BSA free (AB314060)")
![Immunocytochemistry - Anti-AHR antibody [BLR118H] - BSA free (AB314060), expandable thumbnail](https://content.abcam.com/products/images/ahr-antibody-blr118h-bsa-free-ab314060--immunocytochemistry-img420441.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-AHR antibody [BLR118H] - BSA free (AB314060)")
![Western blot - Anti-AHR antibody [BLR118H] - BSA free (AB314060), expandable thumbnail](https://content.abcam.com/products/images/ahr-antibody-blr118h-bsa-free-ab314060--western-blot-img420440.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AHR antibody [BLR118H] - BSA free (AB314060)")
![Immunoprecipitation - Anti-AHR antibody [BLR118H] - BSA free (AB314060), expandable thumbnail](https://content.abcam.com/products/images/ahr-antibody-blr118h-bsa-free-ab314060--immunoprecipitation-img420439.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-AHR antibody [BLR118H] - BSA free (AB314060)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human AHR aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35869
Reactivity data
Select an application| ICC | IHC-P | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed:23275542, PubMed:30373764, PubMed:32818467, PubMed:7961644). Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE) (PubMed:23275542, PubMed:30373764, PubMed:7961644). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (PubMed:12213388). Xenobiotics can act as ligands: upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed:7961644). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed:34521881, PubMed:7961644). Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists (PubMed:18076143). Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands (PubMed:32818467, PubMed:32866000). Acts as a negative regulator of anti-tumor immunity: indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity (PubMed:32818467). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed:28602820). Inhibits PER1 by repressing the CLOCK-BMAL1 heterodimer mediated transcriptional activation of PER1 (PubMed:28602820). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820).
Alternative names
BHLHE76, AHR, Aryl hydrocarbon receptor, Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76
Recommended products
Rabbit Recombinant Monoclonal Aryl hydrocarbon Receptor antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AHR aa 750 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human AHR aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35869
- Clone number
- BLR118H
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Aryl hydrocarbon Receptor (AhR) also known as the Ah receptor is a ligand-activated transcription factor expressed in various tissues including liver lung and skin. The AhR has a molecular mass of approximately 96 kDa. It forms a complex with the aryl hydrocarbon receptor nuclear translocator (ARNT) upon ligand binding. AhR can bind to several compounds including aryl azide influencing the expression of numerous genes involved in xenobiotic metabolism.
- Biological function summary
The AhR plays an important role in the regulation of detoxification enzymes such as cytochrome P450s. This receptor functions as a sensor for environmental toxins activating detoxification pathways once bound to ligands. It also contributes to the modulation of immune responses and development processes. The AhR often forms part of a larger protein complex to exert its effects on gene expression and cellular processes.
- Pathways
The AhR significantly participates in the xenobiotic metabolism pathway and the dioxin signaling pathway. It interacts with proteins such as ARNT and cytochrome P450 enzymes to mediate the cellular response to toxins. The activity of AhR regulates the expression of phase I and phase II detoxification enzymes making it an integral part of the body’s defense against environmental chemicals.
- Associated diseases and disorders
Disturbances in AhR activity have been linked to cancer and autoimmune diseases. Altered expression or function of AhR can contribute to aberrant cell proliferation and immune dysregulation. The AhR interacts with other proteins like peroxidase and its substrates playing a role in oxidative stress responses associated with these conditions. Understanding these links can offer insights into therapeutic targets for related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHR antibody [BLR118H] - BSA free (ab314060), expandable thumbnail](https://content.abcam.com/products/images/ahr-antibody-blr118h-bsa-free-ab314060--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420442.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHR antibody [BLR118H] - BSA free (ab314060)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHR antibody [BLR118H] - BSA free (ab314060)
Detection of human AHR in FFPE bladder carcinoma by immunohistochemistry Antibody:Rabbit anti-AHR recombinant monoclonal antibody [BLR118H] (A700-118 Lot 1). Secondary:HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.
![Immunocytochemistry - Anti-AHR antibody [BLR118H] - BSA free (ab314060), expandable thumbnail](https://content.abcam.com/products/images/ahr-antibody-blr118h-bsa-free-ab314060--immunocytochemistry-img420441.jpg "Immunocytochemistry - Anti-AHR antibody [BLR118H] - BSA free (ab314060)")
Immunocytochemistry - Anti-AHR antibody [BLR118H] - BSA free (ab314060)
Detection of human AHR in FFPE GaMG cells by immunocytochemistry. Antibody:Rabbit anti-AHR recombinant monoclonal antibody [BLR118H] (A700-118 Lot 1). Secondary:HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.
![Western blot - Anti-AHR antibody [BLR118H] - BSA free (ab314060), expandable thumbnail](https://content.abcam.com/products/images/ahr-antibody-blr118h-bsa-free-ab314060--western-blot-img420440.jpg "Western blot - Anti-AHR antibody [BLR118H] - BSA free (ab314060)")
Western blot - Anti-AHR antibody [BLR118H] - BSA free (ab314060)
Samples:Whole cell lysate (50 µg) from KM12, HeLa, Hep-G2, HEK293T, GaMG, RT-4, Malme-3M, and MCF-7 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-AHR recombinant monoclonal antibody [BLR118H] (A700-118 Lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).
All lanes: Western blot - Anti-AHR antibody [BLR118H] - BSA free (ab314060) at 1/1000 dilution
Lane 1: KM12 Whole cell lysate at 50 µg
Lane 2: HeLa Whole cell lysate at 50 µg
Lane 3: Hep-G2 Whole cell lysate at 50 µg
Lane 4: HEK293T Whole cell lysate at 50 µg
Lane 5: GaMG Whole cell lysate at 50 µg
Lane 6: RT-4 Whole cell lysate at 50 µg
Lane 7: Malme-3M Whole cell lysate at 50 µg
Lane 8: MCF-7 Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Immunoprecipitation - Anti-AHR antibody [BLR118H] - BSA free (ab314060), expandable thumbnail](https://content.abcam.com/products/images/ahr-antibody-blr118h-bsa-free-ab314060--immunoprecipitation-img420439.jpg "Immunoprecipitation - Anti-AHR antibody [BLR118H] - BSA free (ab314060)")
Immunoprecipitation - Anti-AHR antibody [BLR118H] - BSA free (ab314060)
Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies:Rabbit anti-AHR recombinant monoclonal antibody [BLR118H] (A700-118 Lot 1) used for IP at 20 µl/mg lysate. AHR was also immunoprecipitated by rabbit anti-AHR antibody BL1617B-4H10. For blotting immunoprecipitated AHR, A700-118 was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.
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