AB314060

Anti-AHR antibody [BLR118H] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal Aryl hydrocarbon Receptor antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AHR aa 750 to C-terminus.

View Alternative Names

BHLHE76, AHR, Aryl hydrocarbon receptor, Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHR antibody [BLR118H] - BSA free (AB314060)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHR antibody [BLR118H] - BSA free (AB314060)

Detection of human AHR in FFPE bladder carcinoma by immunohistochemistry Antibody : Rabbit anti-AHR recombinant monoclonal antibody [BLR118H] (A700-118 Lot 1). Secondary : HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-AHR antibody [BLR118H] - BSA free (AB314060)

Detection of human AHR in FFPE GaMG cells by immunocytochemistry. Antibody : Rabbit anti-AHR recombinant monoclonal antibody [BLR118H] (A700-118 Lot 1). Secondary : HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-AHR antibody [BLR118H] - BSA free (AB314060)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-AHR recombinant monoclonal antibody [BLR118H] (A700-118 Lot 1) used for IP at 20 µl/mg lysate. AHR was also immunoprecipitated by rabbit anti-AHR antibody BL1617B-4H10. For blotting immunoprecipitated AHR, A700-118 was used at 1 : 1000. Chemiluminescence with an exposure time of 3 seconds.

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Supplier Data

Western blot - Anti-AHR antibody [BLR118H] - BSA free (AB314060)

Samples : Whole cell lysate (50 µg) from KM12, HeLa, Hep-G2, HEK293T, GaMG, RT-4, Malme-3M, and MCF-7 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-AHR recombinant monoclonal antibody [BLR118H] (A700-118 Lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A).

All lanes:

Western blot - Anti-AHR antibody [BLR118H] - BSA free (ab314060) at 1/1000 dilution

Lane 1:

KM12 Whole cell lysate at 50 µg

Lane 2:

HeLa Whole cell lysate at 50 µg

Lane 3:

Hep-G2 Whole cell lysate at 50 µg

Lane 4:

HEK293T Whole cell lysate at 50 µg

Lane 5:

GaMG Whole cell lysate at 50 µg

Lane 6:

RT-4 Whole cell lysate at 50 µg

Lane 7:

Malme-3M Whole cell lysate at 50 µg

Lane 8:

MCF-7 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR118H

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, WB, ICC

applications

Immunogen

Synthetic Peptide within Human AHR aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P35869

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Aryl hydrocarbon Receptor (AhR) also known as the Ah receptor is a ligand-activated transcription factor expressed in various tissues including liver lung and skin. The AhR has a molecular mass of approximately 96 kDa. It forms a complex with the aryl hydrocarbon receptor nuclear translocator (ARNT) upon ligand binding. AhR can bind to several compounds including aryl azide influencing the expression of numerous genes involved in xenobiotic metabolism.

Biological function summary

The AhR plays an important role in the regulation of detoxification enzymes such as cytochrome P450s. This receptor functions as a sensor for environmental toxins activating detoxification pathways once bound to ligands. It also contributes to the modulation of immune responses and development processes. The AhR often forms part of a larger protein complex to exert its effects on gene expression and cellular processes.

Pathways

The AhR significantly participates in the xenobiotic metabolism pathway and the dioxin signaling pathway. It interacts with proteins such as ARNT and cytochrome P450 enzymes to mediate the cellular response to toxins. The activity of AhR regulates the expression of phase I and phase II detoxification enzymes making it an integral part of the body’s defense against environmental chemicals.

Disturbances in AhR activity have been linked to cancer and autoimmune diseases. Altered expression or function of AhR can contribute to aberrant cell proliferation and immune dysregulation. The AhR interacts with other proteins like peroxidase and its substrates playing a role in oxidative stress responses associated with these conditions. Understanding these links can offer insights into therapeutic targets for related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed : 23275542, PubMed : 30373764, PubMed : 32818467, PubMed : 7961644). Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE) (PubMed : 23275542, PubMed : 30373764, PubMed : 7961644). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (PubMed : 12213388). Xenobiotics can act as ligands : upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed : 7961644, PubMed : 33193710). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed : 34521881, PubMed : 7961644). Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists (PubMed : 18076143). Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands (PubMed : 32818467, PubMed : 32866000). Acts as a negative regulator of anti-tumor immunity : indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity (PubMed : 32818467). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed : 28602820). Inhibits PER1 by repressing the CLOCK-BMAL1 heterodimer mediated transcriptional activation of PER1 (PubMed : 28602820). The heterodimer ARNT : AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed : 28602820).

See full target information AHR

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AHR antibody [BLR118H] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHR antibody [BLR118H] - BSA free (AB314060)

Immunocytochemistry - Anti-AHR antibody [BLR118H] - BSA free (AB314060)

Immunoprecipitation - Anti-AHR antibody [BLR118H] - BSA free (AB314060)

Western blot - Anti-AHR antibody [BLR118H] - BSA free (AB314060)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise