AB231040

Anti-Ahsp antibody

Name: Anti-Ahsp antibody (ab231040) | Abcam
Brand: Abcam Limited
SKU: AB231040
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Erythroid associated factor antibody. Suitable for WB, IHC-P and reacts with Rat, Recombinant full length protein - Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

Ahsp

5 Images

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (AB231040)

Paraffin-embedded rat stomach tissue stained for Ahsp with ab231040 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (AB231040)

Paraffin-embedded rat liver tissue stained for Ahsp with ab231040 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (AB231040)

Paraffin-embedded rat kidney tissue stained for Ahsp with ab231040 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Ahsp antibody (AB231040)

All lanes:

Western blot - Anti-Ahsp antibody (ab231040) at 3 µg/mL

All lanes:

Recombinant rat Ahsp protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution

false

Supplier Data

Western blot - Anti-Ahsp antibody (AB231040)

All lanes:

Western blot - Anti-Ahsp antibody (ab231040) at 3 µg/mL

Lane 1:

Rat liver lysate

Lane 2:

Mouse liver lysate

Secondary

All lanes:

HRP-linked Guinea pig Anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Recombinant full length protein - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231040 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AHSP protein also known as Alpha Hemoglobin Stabilizing Protein plays an important role in the stabilization of alpha-globin chains. This protein has a mass of approximately 11 kDa and is expressed in erythroid cells which are involved in red blood cell development and function. By binding to alpha-globin the AHSP protein assists in preventing harmful aggregation and degradation of these chains which is important in hemoglobin assembly and stability.

Biological function summary

AHSP protein assists in preventing the precipitation of excess free alpha-globin chains within erythroid cells. It acts as a chaperone guiding the proper folding of alpha-globin and facilitating the formation of hemoglobin. This protein does not work alone; it operates as a component of the erythrocytic protein maturation complex working closely with beta-globin during erythropoiesis to ensure effective hemoglobin production.

Pathways

The AHSP protein integrates into cellular processes by playing a role in the hemoglobin synthesis pathway. It is a part of the broader erythroid differentiation pathway and ensures effective erythrocyte maturation and function. AHSP protein is related to HBA1 and HBA2 proteins through these pathways as they are all important components during hemoglobin formation.

Malfunction or reduced expression of the AHSP protein links to various hemoglobinopathies such as beta-thalassemia and alpha-thalassemia. In these conditions imbalances in the globin chains cause abnormal erythrocyte formation and impaired oxygen transport. The AHSP protein connects to HBB and HBA1 proteins when considering these disorders as its stabilizing role influences the equilibrium between alpha- and beta-globin chains important for effective erythrocyte functionality.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Erythroid associated factor

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 9: PubMed34944635

2021

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuying Liang,Haijing Guan,Jingwen Sun,Yan Qi,Wenjuan Yao

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ahsp antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (AB231040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (AB231040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody (AB231040)

Western blot - Anti-Ahsp antibody (AB231040)

Western blot - Anti-Ahsp antibody (AB231040)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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