AB250261

Anti-Ahsp antibody [EPR12319] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal AHSP antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

EDRF, ERAF, AHSP, Alpha-hemoglobin-stabilizing protein, Erythroid differentiation-related factor, Erythroid-associated factor

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] - BSA and Azide free (AB250261)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] - BSA and Azide free (AB250261)

This data was developed using ab180861, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Ahsp with ab180861 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] - BSA and Azide free (AB250261)

This data was developed using ab180861, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human fetal spleen tissue labeling Ahsp with ab180861 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Ahsp antibody [EPR12319] - BSA and Azide free (AB250261)

This data was developed using ab180861, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab180861 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Ahsp antibody [EPR12319] (<a href='/en-us/products/primary-antibodies/ahsp-antibody-epr12319-ab180861'>ab180861</a>)

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-Ahsp antibody [EPR12319] - BSA and Azide free (AB250261)

This data was developed using ab180861, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ahsp antibody [EPR12319] (<a href='/en-us/products/primary-antibodies/ahsp-antibody-epr12319-ab180861'>ab180861</a>) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Predicted band size: 12 kDa

false

Unconjugated

Anti-Ahsp antibody [EPR12319]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12319

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250261 is the carrier-free version of ab180861.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AHSP protein also known as Alpha Hemoglobin Stabilizing Protein plays an important role in the stabilization of alpha-globin chains. This protein has a mass of approximately 11 kDa and is expressed in erythroid cells which are involved in red blood cell development and function. By binding to alpha-globin the AHSP protein assists in preventing harmful aggregation and degradation of these chains which is important in hemoglobin assembly and stability.

Biological function summary

AHSP protein assists in preventing the precipitation of excess free alpha-globin chains within erythroid cells. It acts as a chaperone guiding the proper folding of alpha-globin and facilitating the formation of hemoglobin. This protein does not work alone; it operates as a component of the erythrocytic protein maturation complex working closely with beta-globin during erythropoiesis to ensure effective hemoglobin production.

Pathways

The AHSP protein integrates into cellular processes by playing a role in the hemoglobin synthesis pathway. It is a part of the broader erythroid differentiation pathway and ensures effective erythrocyte maturation and function. AHSP protein is related to HBA1 and HBA2 proteins through these pathways as they are all important components during hemoglobin formation.

Malfunction or reduced expression of the AHSP protein links to various hemoglobinopathies such as beta-thalassemia and alpha-thalassemia. In these conditions imbalances in the globin chains cause abnormal erythrocyte formation and impaired oxygen transport. The AHSP protein connects to HBB and HBA1 proteins when considering these disorders as its stabilizing role influences the equilibrium between alpha- and beta-globin chains important for effective erythrocyte functionality.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia.

See full target information AHSP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ahsp antibody [EPR12319] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] - BSA and Azide free (AB250261)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] - BSA and Azide free (AB250261)

Immunoprecipitation - Anti-Ahsp antibody [EPR12319] - BSA and Azide free (AB250261)

Western blot - Anti-Ahsp antibody [EPR12319] - BSA and Azide free (AB250261)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise