Knockout Tested Rabbit Recombinant Monoclonal AICDA antibody. Carrier free. Suitable for IP, ChIP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![ChIP - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--chip-img120606.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126676.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457)")
![Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunoprecipitation-img8764.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457)")
![Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunoprecipitation-img3323.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125413.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (AB269457)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ChIP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
8 products for Alternative Version
Target data
Function
Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses (PubMed:18722174, PubMed:21385873, PubMed:21518874, PubMed:27716525). May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (PubMed:21496894).
Alternative names
AID, AICDA, Single-stranded DNA cytosine deaminase, Activation-induced cytidine deaminase, Cytidine aminohydrolase
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal AICDA antibody. Carrier free. Suitable for IP, ChIP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23436-45
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab269457 is the carrier-free version of Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AICDA also known as activation-induced cytidine deaminase is an enzyme with a molecular mass of approximately 25 kDa. It is expressed in germinal center B cells within the lymphoid tissues particularly in the spleen and lymph nodes. AICDA catalyzes the deamination of cytidine to uridine in single-stranded DNA an essential step in the processes of somatic hypermutation and class switch recombination. These mechanisms are important for antibody diversity and specificity in the adaptive immune response.
- Biological function summary
AICDA plays an important role in the adaptive immune system by facilitating the diversification of antibodies. It does not appear to function as part of a larger protein complex but operates independently to induce mutations in variable regions of immunoglobulin genes. This activity enables B cells to produce antibodies with higher affinity and different isotypes important for an effective immune defense system. AICDA’s function ensures the generation of diverse antibody repertoires allowing the immune system to effectively target a wide range of pathogens.
- Pathways
AICDA is integral to the immunoglobulin gene diversification pathway. It initiates somatic hypermutation and class switch recombination by deaminating cytosine residues in the DNA of immunoglobulin genes. This action relates AICDA to other proteins involved in DNA repair and error-prone replication such as uracil-DNA glycosylase (UNG) and the mismatch repair proteins like MSH2 and MSH6. AICDA's deamination activity triggers the recruitment of these proteins to introduce mutations and remove uracil residues facilitating the generation of antibody diversity.
- Associated diseases and disorders
Aberrant AICDA activity associates with lymphomas and autoimmune conditions. For example dysregulation of AICDA could contribute to oncogenic mutations leading to non-Hodgkin's lymphoma. Additionally incorrect or unscheduled activation of AICDA might result in the production of autoantibodies implicated in autoimmune disorders like systemic lupus erythematosus (SLE). AICDA’s interaction with DNA repair proteins during misguided activation may lead to genomic instability linking it to both tumorigenesis and autoimmunity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![ChIP - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--chip-img120606.jpg "ChIP - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
ChIP - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)
Chromatin was prepared from Ramos cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 (red), or 5 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are commercial primers from paper: PMC2905439
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126676.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)
Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma tissue labeling AICDA with Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 at 1/4000 dilution (0.12ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Mainly cytoplasmic staining (weak nuclear staining) in part of tumor cells of human diffuse large B-cell lymphoma (PMID: 29251015).
The section was incubated with Anti-BAP1 antibody [EPR22826-65] ab255611 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454).
![Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunoprecipitation-img8764.jpg "Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)
AICDA was immunoprecipitated from 0.35 mg NAMALWA (human Burkitt's lymphoma B lymphocyte) whole cell lysate with Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NAMALWA whole cell lysate 10ug.
Lane 2: Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 IP in NAMALWA whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 in NAMALWA whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 90 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454).
All lanes: Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454)
Predicted band size: 23 kDa
Observed band size: 24 kDa
![Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunoprecipitation-img3323.jpg "Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)
AICDA was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Ramos whole cell lysate 10ug.
Lane 2: Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 IP in Ramos whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 in Ramos whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 90 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454).
All lanes: Immunoprecipitation - Anti-AICDA antibody [EPR23436-45] - ChIP Grade (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454)
Predicted band size: 23 kDa
Observed band size: 24 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125413.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)
Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling AICDA with Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 at 1/4000 dilution (0.12ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Mainly cytoplasmic staining (weak nuclear staining) in part of tumor cells of human Hodgkin lymphoma (PMID: 15732141).
The section was incubated with Anti-BAP1 antibody [EPR22826-65] ab255611 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6714.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling AICDA with Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 at 1/4000 dilution (0.12ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Mainly cytoplasmic staining (weak nuclear staining) in germinal center cells of human tonsil (PMID:23877718, 15732141, PMID: 29251015).
The section was incubated with Anti-BAP1 antibody [EPR22826-65] ab255611 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454).
![Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--flow-cytometry-intracellular-img71378.jpg "Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / NAMALWA (human Burkitt's lymphoma B lymphocyte, Right) cells labelling AICDA with Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Blac) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control:K-562 cell line (PMID: 27217538).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454).
![Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--flow-cytometry-intracellular-img414640.jpg "Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Ramos (human Burkitt's lymphoma B lymphocyte) cells labelling AICDA with Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454).
![Western blot - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--western-blot-img418061.jpg "Western blot - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
Western blot - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454). False colour image of Western blot: Anti-AICDA antibody [EPR23436-45] - ChIP Grade staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454 was shown to bind specifically to AICDA. A band was observed at 22 kDa in wild-type Raji cell lysates with no signal observed at this size in AICDA knockout cell line ab277185 (knockout cell lysate ab277227). To generate this image, wild-type and AICDA knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-AICDA antibody [EPR23436-45] - ChIP Grade (Anti-AICDA antibody [EPR23436-45] - ChIP Grade ab269454) at 1/1000 dilution
Lane 1: Wild-type Raji cell lysate at 20 µg
Lane 2: AICDA knockout Raji cell lysate at 20 µg
Lane 3: Daudi cell lysate at 20 µg
Lane 4: SH-SY5Y cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 22 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6714.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
![Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--flow-cytometry-intracellular-img71378.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
![Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--flow-cytometry-intracellular-img414640.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
![Western blot - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457), expandable thumbnail](https://content.abcam.com/products/images/aicda-antibody-epr23436-45-chip-grade-bsa-and-azide-free-ab269457--western-blot-img418061.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AICDA antibody [EPR23436-45] - ChIP Grade - BSA and Azide free (ab269457)")
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