Anti-AICDA antibody [JUA51E]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [JUA51E] (AB323835)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling AICDA with ab323835 at 1/200 (5.45 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human testis. The primary antibody was incubated for 30 mins at room temperature, then incubation with the post primary antibody ab125913 at 1/1000 for 8 mins at room temperature, then incubation with the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [JUA51E] (AB323835)

Immunohistochemical analysis of paraffin-embedded Human Burkitt lymphoma tissue labeling AICDA with ab323835 at 1/200 (5.45 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human Burkitt lymphoma. The primary antibody was incubated for 30 mins at room temperature, then incubation with the post primary antibody ab125913 at 1/1000 for 8 mins at room temperature, then incubation with the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AICDA antibody [JUA51E] (AB323835)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling AICDA with ab323835 at 1/200 (5.45 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil. The primary antibody was incubated for 30 mins at room temperature, then incubation with the post primary antibody ab125913 at 1/1000 for 8 mins at room temperature, then incubation with the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-AICDA antibody [JUA51E] (AB323835)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling AICDA with ab323835 at 1/100 (10 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in Raji cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : K-562.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 (10 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab323835 at a 1/100 dilution followed by ab150088 at a 1/1000 dilution.

-ve control 2 : ab179513 at a 1/200 dilution followed by ab150117 at a 1/1000 dilution.

• WB

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Western blot - Anti-AICDA antibody [JUA51E] (AB323835)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HL-60, K-562 (PMID : 27217538).

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-AICDA antibody [JUA51E] (ab323835) at 1/1000 dilution

Lane 1:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 4:

HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 22 kDa,36 kDa

true

Exposure time: 114s