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AB199323

Anti-AIDA antibody [EPR19161]

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(1 Publication)

Rabbit Recombinant Monoclonal AIDA antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Axin interaction partner and dorsalization antagonist, Aida

2 Images
Western blot - Anti-AIDA antibody [EPR19161] (AB199323)
  • WB

Supplier Data

Western blot - Anti-AIDA antibody [EPR19161] (AB199323)

Blocking buffer : 5% NFDM/TBST; Dilution buffer : 5% BSA/TBST.

The data was kindly provided by our collaborator Dr. Sheng-Cai Lin, Xiamen University.

All lanes:

Western blot - Anti-AIDA antibody [EPR19161] (ab199323) at 1/500 dilution

Lane 1:

Immortalized wild type MEF (mouse embryonic fibroblast cell line), whole cell lysate at 15 µg

Lane 2:

Aida knockout immortalized MEF, whole cell lysate at 15 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/5000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa

true

Exposure time: 30s

Western blot - Anti-AIDA antibody [EPR19161] (AB199323)
  • WB

Supplier Data

Western blot - Anti-AIDA antibody [EPR19161] (AB199323)

Blocking buffer : 5% NFDM/TBST; Dilution buffer : 5% BSA/TBST.

The data was kindly provided by our collaborator Dr. Sheng-Cai Lin, Xiamen University.

All lanes:

Western blot - Anti-AIDA antibody [EPR19161] (ab199323) at 1/500 dilution

Lane 1:

Wild type mouse lung homogenate at 50 µg

Lane 2:

Aida knockout mouse lung homogenate at 50 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/5000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa

true

Exposure time: 15s

  • Carrier free

    Anti-AIDA antibody [EPR19161] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19161

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p>Under our experimental conditions ab199323 detected non-specific bands in WB. However, the band of interest at ~36kDa disappears from the knockout sample demonstrating the specificity of the antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AIDA protein also known as Anisomycin-induced protein A is a little-studied member of a protein family yet plays significant mechanical roles in cellular activities. Its molecular mass is not well characterized but researchers have noted its expression across various tissues notably in the brain and immune cells. The presence in these tissues suggests a versatile and potentially significant function in cellular processes awaiting further exploration.
Biological function summary

The AIDA protein interacts with multiple cellular components to modulate immune responses. It likely forms part of a multi-protein complex orchestrating signaling processes pivotal for cell communication and response. The protein appears to regulate the transcription of certain genes implicated in stress responses offering insights into its possible regulatory roles within cells.

Pathways

AIDA is integrated into the MAPK signaling pathway which is important for transmitting cellular responses to stress stimuli. AIDA interacts with other proteins such as ERK and JNK within this pathway demonstrating its involvement in modulating cellular reactions to external stimuli. This association highlights the dynamic role of AIDA in adapting cellular states to environmental conditions.

AIDA's function relates significantly to neuroinflammatory conditions and immune dysregulation. Its interaction with proteins like NF-kB suggests a role in pathogenesis where chronic inflammation is prevalent such as in multiple sclerosis and rheumatoid arthritis. These associations emphasize the potential of AIDA as a target for therapeutic interventions in inflammatory diseases promising advances for treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a ventralizing factor during embryogenesis (By similarity). Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
See full target information Aida
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 27:843-853.e6 PubMed29617643

2018

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Luo,Ming Jiang,Guili Lian,Qing Liu,Meng Shi,Terytty Yang Li,Lintao Song,Jing Ye,Ying He,Luming Yao,Cixiong Zhang,Zhi-Zhong Lin,Chen-Song Zhang,Tong-Jin Zhao,Wei-Ping Jia,Peng Li,Shu-Yong Lin,Sheng-Cai Lin
View all publications
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Product promise

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