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AB274421

Anti-AIDA antibody [EPR20903-53] - BSA and Azide free

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Rabbit Recombinant Monoclonal AIDA antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.

View Alternative Names

C1orf80, AIDA, Axin interaction partner and dorsalization antagonist

1 Images
Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (AB274421)
  • WB

Lab

Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (AB274421)

This data was developed using ab234419, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

ab234419 was shown to specifically react with AIDA in wild-type MEF cells as signal was lost in AIDA knockout cells. Wild-type and AIDA knockout samples were subjected to SDS-PAGE. ab234419 and ab52901 (Rabbit anti-beta Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50, 000 dilution and 1/100,000 dilution for 1 hour at room temperature before imaging respectively. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

AIDA knockout MEF cell lysate was kindly provided by our collaborator.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-AIDA antibody [EPR20903-53] (<a href='/en-us/products/primary-antibodies/aida-antibody-epr20903-53-ab234419'>ab234419</a>) at 1/1000 dilution

Lane 1:

Wild-type MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 40 µg

Lane 2:

AIDA knockout MEF whole cell lysate at 40 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

Lane 4:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 40 µg

Lane 5:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 40 µg

Lane 6:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 7:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 40 µg

Lane 8:

C6 (rat glial tumor glial cell) whole cell lysate at 40 µg

Lane 9:

PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20903-53

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab274421 is the carrier-free version of ab234419.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AIDA protein also known as Anisomycin-induced protein A is a little-studied member of a protein family yet plays significant mechanical roles in cellular activities. Its molecular mass is not well characterized but researchers have noted its expression across various tissues notably in the brain and immune cells. The presence in these tissues suggests a versatile and potentially significant function in cellular processes awaiting further exploration.
Biological function summary

The AIDA protein interacts with multiple cellular components to modulate immune responses. It likely forms part of a multi-protein complex orchestrating signaling processes pivotal for cell communication and response. The protein appears to regulate the transcription of certain genes implicated in stress responses offering insights into its possible regulatory roles within cells.

Pathways

AIDA is integrated into the MAPK signaling pathway which is important for transmitting cellular responses to stress stimuli. AIDA interacts with other proteins such as ERK and JNK within this pathway demonstrating its involvement in modulating cellular reactions to external stimuli. This association highlights the dynamic role of AIDA in adapting cellular states to environmental conditions.

AIDA's function relates significantly to neuroinflammatory conditions and immune dysregulation. Its interaction with proteins like NF-kB suggests a role in pathogenesis where chronic inflammation is prevalent such as in multiple sclerosis and rheumatoid arthritis. These associations emphasize the potential of AIDA as a target for therapeutic interventions in inflammatory diseases promising advances for treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling (By similarity).
See full target information AIDA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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