Rabbit Recombinant Monoclonal AIDA antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (AB274421), expandable thumbnail](https://content.abcam.com/products/images/aida-antibody-epr20903-53-bsa-and-azide-free-ab274421--western-blot-img7322.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (AB274421)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling (By similarity).
Alternative names
C1orf80, AIDA, Axin interaction partner and dorsalization antagonist
Recommended products
Rabbit Recombinant Monoclonal AIDA antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20903-53
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab274421 is the carrier-free version of Anti-AIDA antibody [EPR20903-53] ab234419.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The AIDA protein also known as Anisomycin-induced protein A is a little-studied member of a protein family yet plays significant mechanical roles in cellular activities. Its molecular mass is not well characterized but researchers have noted its expression across various tissues notably in the brain and immune cells. The presence in these tissues suggests a versatile and potentially significant function in cellular processes awaiting further exploration.
- Biological function summary
The AIDA protein interacts with multiple cellular components to modulate immune responses. It likely forms part of a multi-protein complex orchestrating signaling processes pivotal for cell communication and response. The protein appears to regulate the transcription of certain genes implicated in stress responses offering insights into its possible regulatory roles within cells.
- Pathways
AIDA is integrated into the MAPK signaling pathway which is important for transmitting cellular responses to stress stimuli. AIDA interacts with other proteins such as ERK and JNK within this pathway demonstrating its involvement in modulating cellular reactions to external stimuli. This association highlights the dynamic role of AIDA in adapting cellular states to environmental conditions.
- Associated diseases and disorders
AIDA's function relates significantly to neuroinflammatory conditions and immune dysregulation. Its interaction with proteins like NF-kB suggests a role in pathogenesis where chronic inflammation is prevalent such as in multiple sclerosis and rheumatoid arthritis. These associations emphasize the potential of AIDA as a target for therapeutic interventions in inflammatory diseases promising advances for treatment strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421), expandable thumbnail](https://content.abcam.com/products/images/aida-antibody-epr20903-53-bsa-and-azide-free-ab274421--western-blot-img7322.jpg "Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)")
Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)
This data was developed using Anti-AIDA antibody [EPR20903-53] ab234419, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-AIDA antibody [EPR20903-53] ab234419 was shown to specifically react with AIDA in wild-type MEF cells as signal was lost in AIDA knockout cells. Wild-type and AIDA knockout samples were subjected to SDS-PAGE. Anti-AIDA antibody [EPR20903-53] ab234419 and Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker ab52901 (Rabbit anti-beta Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/50, 000 dilution and 1/100,000 dilution for 1 hour at room temperature before imaging respectively. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
AIDA knockout MEF cell lysate was kindly provided by our collaborator.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-AIDA antibody [EPR20903-53] (Anti-AIDA antibody [EPR20903-53] ab234419) at 1/1000 dilution
Lane 1: Wild-type MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate at 40 µg
Lane 2: AIDA knockout MEF whole cell lysate at 40 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg
Lane 4: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 40 µg
Lane 5: C2C12 (mouse myoblasts myoblast) whole cell lysate at 40 µg
Lane 6: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg
Lane 7: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 40 µg
Lane 8: C6 (rat glial tumor glial cell) whole cell lysate at 40 µg
Lane 9: PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 40 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa
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