AB110327

Anti-AIF antibody [7F7AB10] - Mitochondrial Marker

Name: Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (ab110327) | Abcam
Brand: Abcam Limited
SKU: AB110327
Price: 470 USD
Availability: InStock
Rating: 4 (1 reviews)

BOND RX™ Validated
What is this?

(1 Review)

(6 Publications)

Mouse Monoclonal AIF antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

AIF, PDCD8, AIFM1, Programmed cell death protein 8

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

IHC image of AIF staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110327, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

Immunocytochemistry analysis using ab110327 at 5μg/ml staining AIF in HeLa cells (4% paraformaldehyde fixed and Triton X-100 permeabilized). The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

Flow Cytometry - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

Flow Cyt

Unknown

Flow Cytometry - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

Flow cytometric analysis using ab110327 at 1µg/ml staining AIF in HL60 cells (blue). Isotype control antibody (red).

Unknown

Western blot - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

All lanes:

Western blot - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (ab110327) at 5 µg/mL

All lanes:

Isolated mitochondria from Human heart at 5 µg

Predicted band size: 67 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-AIF antibody [7F7AB10] - Mitochondrial Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7F7AB10

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB, IHC-P, ICC/IF

applications

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-5 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-10 µg/mL", "ICCIF-species-notes": "For 2 hours.Paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes)." } } }

style

left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

ab110327 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AIF short for Apoptosis-Inducing Factor is a flavoprotein characterized by its mass of approximately 57 kDa. It resides in the mitochondria of cells where it plays a decisive role in apoptosis. Under normal conditions AIF remains in the mitochondrial intermembrane space but when cells receive apoptotic signals AIF translocates to the nucleus leading to chromatin condensation and large-scale DNA fragmentation. The protein is also known as AIFM1 and performs functions beyond apoptosis including regulation of reactive oxygen species. Often analyzed using tools like a mitochondrial AB marker researchers use AIF as an important part of understanding cell death mechanisms.

Biological function summary

AIF functions as an important element in mitochondrial-mediated apoptosis. Once released from mitochondria AIF triggers a caspase-independent pathway of apoptosis making it distinct from other pathways where caspases are central. While not part of a larger protein complex AIF interacts closely with several mitochondrial and nuclear elements to execute its functions. Researchers often monitor AIF activity using apoptosis ELISA kits which help in precise detection and quantification of this protein within cellular systems.

Pathways

The presence and activity of AIF align with the broader apoptotic pathway and mitochondrial respiration. AIF links significantly with other molecules like cytochrome c released during apoptosis although cytochrome c follows a caspase-dependent pathway. It also shows involvement in various cellular injury and stress response pathways reinforcing its role as a major player in cellular fate decisions and intrinsic apoptotic mechanisms.

AIF holds relevance in neurodegenerative disorders such as Parkinson's disease and certain forms of cancer. Misregulation or mutations in AIF can lead to increased susceptibility to these conditions highlighting its importance in maintaining normal cellular and mitochondrial function. AIF also interacts with other proteins implicated in these disorders such as E2F1 in cancer which shares similar apoptotic regulatory roles. These interactions highlight the biological significance of AIF beyond its fundamental apoptosis function making it a potential target for therapeutic interventions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions both as NADH oxidoreductase and as regulator of apoptosis (PubMed : 17094969, PubMed : 20362274, PubMed : 23217327, PubMed : 33168626). In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway (PubMed : 20362274). Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (By similarity). The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (PubMed : 20362274). Binds to DNA in a sequence-independent manner (PubMed : 27178839). Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (PubMed : 17094969). Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (PubMed : 19418225). In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed : 26004228).. Isoform 4. Has NADH oxidoreductase activity. Does not induce nuclear apoptosis.. Isoform 5. Pro-apoptotic isoform.

See full target information AIFM1

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 95:e0203020 PubMed33952640

2021

A Human Endogenous Bornavirus-Like Nucleoprotein Encodes a Mitochondrial Protein Associated with Cell Viability.

Applications

Unspecified application

Species

Unspecified reactive species

Kan Fujino,Masayuki Horie,Shohei Kojima,Sae Shimizu,Aya Nabekura,Hiroko Kobayashi,Akiko Makino,Tomoyuki Honda,Keizo Tomonaga

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:2002874 PubMed33898171

2021

OTUD1 Activates Caspase-Independent and Caspase-Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyu Luo,Xiaowei Wu,Pengfei Zhao,Yabing Nan,Wan Chang,Xiaolin Zhu,Dan Su,Zhihua Liu

Frontiers in veterinary science 7:580530 PubMed33263014

2020

Benzyl Isothiocyanate Induces Apoptosis and Inhibits Tumor Growth in Canine Mammary Carcinoma via Downregulation of the Cyclin B1/Cdk1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Cheng,Hongxiu Diao,Zhaoyan Lin,Jiafeng Gao,Ying Zhao,Weijiao Zhang,Qi Wang,Jiahao Lin,Di Zhang,Yipeng Jin,Yongping Bao,Degui Lin

Journal of neurochemistry 151:732-748 PubMed31553812

2019

Subcellular NAMPT-mediated NAD salvage pathways and their roles in bioenergetics and neuronal protection after ischemic injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowan Wang,Zhe Zhang,Nannan Zhang,Hailong Li,Li Zhang,Christopher P Baines,Shinghua Ding

International journal of molecular sciences 20: PubMed30754623

2019

Parkin Interacts with Apoptosis-Inducing Factor and Interferes with Its Translocation to the Nucleus in Neuronal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marianna Guida,Alessandra Zanon,Luigi Montibeller,Alexandros A Lavdas,Judith Ladurner,Francesca Pischedda,Aleksandar Rakovic,Francisco S Domingues,Giovanni Piccoli,Christine Klein,Peter P Pramstaller,Andrew A Hicks,Irene Pichler

Scientific reports 7:4331 PubMed28659599

2017

PARP-1 overexpression contributes to Cadmium-induced death in rat proximal tubular cells via parthanatos and the MAPK signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tongwang Luo,Yan Yuan,Qi Yu,Gang Liu,Mengfei Long,Kanglei Zhang,Jianchun Bian,Jianhong Gu,Hui Zou,Yi Wang,Jiaqiao Zhu,Xuezhong Liu,Zongping Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AIF antibody [7F7AB10] - Mitochondrial Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

Flow Cytometry - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

Western blot - Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB110327)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise