AB228004

Anti-AIMP2/p38 antibody

Name: Anti-AIMP2/p38 antibody (ab228004) | Abcam
Brand: Abcam Limited
SKU: AB228004
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal AIMP2/p38 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AIMP2.

View Alternative Names

JTV1, PRO0992, AIMP2, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, Multisynthase complex auxiliary component p38, Protein JTV-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody (AB228004)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody (AB228004)

Paraffin-embedded human kidney tissue stained for AIMP2/p38 with ab228004 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-AIMP2/p38 antibody (AB228004)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AIMP2/p38 antibody (AB228004)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AIMP2/p38 (green) using ab228004 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-AIMP2/p38 antibody (AB228004)

10% SDS-PAGE

All lanes:

Western blot - Anti-AIMP2/p38 antibody (ab228004) at 1/1000 dilution

All lanes:

HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 30 µg

Predicted band size: 35 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human AIMP2. The exact immunogen used to generate this antibody is proprietary information.

Q13155

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AIMP2 also known as p38 is a multifunctional protein involved in several cellular processes. Measuring at a molecular weight of approximately 52 kDa AIMP2 plays an important role in regulating protein synthesis as a part of the aminoacyl-tRNA synthetase complex. This protein is widely expressed throughout various tissues in the body including lung liver and brain indicating its importance across different physiological contexts.

Biological function summary

AIMP2 acts as a scaffolding protein integrating signals between the aminoacyl-tRNA synthetase complex and other cellular mechanisms. It forms a complex with several components in the multi-aminoacyl-tRNA synthetase complex aiding in protein translation. The protein is involved in the maintenance of cellular homeostasis and stress responses by modulating protein synthesis and degradation processes.

Pathways

Multiple studies have shown that AIMP2 integrates into several important signaling pathways like the p53 pathway. AIMP2 enhances the activation of p53 tumor suppressor protein facilitating cellular responses to DNA damage and oxidative stress. It interacts closely with proteins like MDM2 mediating p53 stability and activity linking AIMP2 to cellular growth control and apoptosis regulation.

Mutations or dysregulation of AIMP2 have been connected to various conditions including neurodegenerative diseases and cancer. For example alterations in AIMP2 expression levels are implicated in Parkinson’s disease where its role in dopaminergic neuron survival is compromised. In cancer AIMP2 interacts with proteins like FBP1 affecting tumor progression and metastasis making it an important target for therapeutic interventions in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for assembly and stability of the aminoacyl-tRNA synthase complex (PubMed : 19131329). Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.

See full target information AIMP2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Viruses 15: PubMed36851687

2023

HIV-1 Gag Binds the Multi-Aminoacyl-tRNA Synthetase Complex via the EPRS Subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Danni Jin,Yiping Zhu,Heidi L Schubert,Stephen P Goff,Karin Musier-Forsyth

The Journal of pharmacy and pharmacology 70:1369-1377 PubMed29943422

2018

Tanshinone IIA effects on ovarian cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Li,Liang Yang,Baolian Zhang,Suqin Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AIMP2/p38 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody (AB228004)

Immunocytochemistry/ Immunofluorescence - Anti-AIMP2/p38 antibody (AB228004)

Western blot - Anti-AIMP2/p38 antibody (AB228004)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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