Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)

Rabbit Recombinant Monoclonal AIMP2/p38 antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (AB316938), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr
Human	Tested	Tested	Tested	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended
Rat	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Target data

See full target information Aminoacyl tRNA synthase complex-interacting multifunctional protein 2

Function

Required for assembly and stability of the aminoacyl-tRNA synthase complex (PubMed:19131329). Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.

Alternative names

JTV1, PRO0992, AIMP2, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, Multisynthase complex auxiliary component p38, Protein JTV-1

Recommended products

Rabbit Recombinant Monoclonal AIMP2/p38 antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR29127-48
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab316938 is the carrirer-free version of Anti-AIMP2/p38 antibody [EPR29127-48] ab316937.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

AIMP2 also known as p38 is a multifunctional protein involved in several cellular processes. Measuring at a molecular weight of approximately 52 kDa AIMP2 plays an important role in regulating protein synthesis as a part of the aminoacyl-tRNA synthetase complex. This protein is widely expressed throughout various tissues in the body including lung liver and brain indicating its importance across different physiological contexts.

Biological function summary

AIMP2 acts as a scaffolding protein integrating signals between the aminoacyl-tRNA synthetase complex and other cellular mechanisms. It forms a complex with several components in the multi-aminoacyl-tRNA synthetase complex aiding in protein translation. The protein is involved in the maintenance of cellular homeostasis and stress responses by modulating protein synthesis and degradation processes.

Pathways

Multiple studies have shown that AIMP2 integrates into several important signaling pathways like the p53 pathway. AIMP2 enhances the activation of p53 tumor suppressor protein facilitating cellular responses to DNA damage and oxidative stress. It interacts closely with proteins like MDM2 mediating p53 stability and activity linking AIMP2 to cellular growth control and apoptosis regulation.

Associated diseases and disorders

Mutations or dysregulation of AIMP2 have been connected to various conditions including neurodegenerative diseases and cancer. For example alterations in AIMP2 expression levels are implicated in Parkinson’s disease where its role in dopaminergic neuron survival is compromised. In cancer AIMP2 interacts with proteins like FBP1 affecting tumor progression and metastasis making it an important target for therapeutic interventions in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling AIMP2/p38 with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat liver. The section was incubated with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling AIMP2/p38 with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum. The section was incubated with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling AIMP2/p38 with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse liver. The section was incubated with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling AIMP2/p38 with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum (PMID: 23974709). The section was incubated with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreatic carcinoma tissue labeling AIMP2/p38 with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human pancreatic carcinoma. The section was incubated with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling AIMP2/p38 with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human breast carcinoma. The section was incubated with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling AIMP2/p38 with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum. The section was incubated with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-AIMP2/p38 antibody [EPR29127-48] (Anti-AIMP2/p38 antibody [EPR29127-48] ab316937) at 1/1000 dilution
Lane 1: Human liver cancer tissue lysate at 30 µg with 5% NFDM/TBST
Lane 2: Mouse brain tissue lysate at 30 µg with 5% NFDM/TBST
Lane 3: Rat brain tissue lysate at 30 µg with 5% NFDM/TBST
Lane 4: Rat liver tissue lysate at 30 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 35 kDa
Exposure time: 48s
Western blot - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
All lanes: Western blot - Anti-AIMP2/p38 antibody [EPR29127-48] (Anti-AIMP2/p38 antibody [EPR29127-48] ab316937) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: HeLa transfected with siRNA specifically targeting AIMP2 whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 35 kDa, 15 kDa
Exposure time: 180s
Western blot - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-AIMP2/p38 antibody [EPR29127-48] (Anti-AIMP2/p38 antibody [EPR29127-48] ab316937) at 1/1000 dilution
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 4: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 35 kDa
Exposure time: 180s
Immunoprecipitation - Anti-AIMP2/p38 antibody [EPR29127-48] - BSA and Azide free (ab316938)
This data was developed using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937, the same antibody clone in a different buffer formulation.
AIMP2/p38 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2: Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-AIMP2/p38 antibody [EPR29127-48] ab316937 in HeLa whole cell lysate
All lanes: Immunoprecipitation - Anti-AIMP2/p38 antibody [EPR29127-48] (Anti-AIMP2/p38 antibody [EPR29127-48] ab316937) at 1/30 dilution
All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 41s