AB155689

Anti-AIMP3/p18 antibody

Name: Anti-AIMP3/p18 antibody (ab155689) | Abcam
Brand: Abcam Limited
SKU: AB155689

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(2 Publications)

Rabbit Polyclonal AIMP3/p18 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EEF1E1 aa 1-200.

View Alternative Names

AIMP3, P18, EEF1E1, Eukaryotic translation elongation factor 1 epsilon-1, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Elongation factor p18, Multisynthase complex auxiliary component p18

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (AB155689)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (AB155689)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling AIMP3/p18 with ab155689 at 1/200 dilution. Lower image is merged with DNA probe.

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Western blot - Anti-AIMP3/p18 antibody (AB155689)

12% SDS PAGE

All lanes:

Western blot - Anti-AIMP3/p18 antibody (ab155689) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 19 kDa

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Western blot - Anti-AIMP3/p18 antibody (AB155689)

12% SDS PAGE

All lanes:

Western blot - Anti-AIMP3/p18 antibody (ab155689) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 19 kDa

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Lab

Western blot - Anti-AIMP3/p18 antibody (AB155689)

All lanes:

Western blot - Anti-AIMP3/p18 antibody (ab155689) at 1/500 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

AIMP3/p18 Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 19 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human EEF1E1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O43324

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AIMP3 also known as p18 or eEF1A1 is a protein that plays an important role in cellular processes. It has a molecular mass of approximately 18 kDa. AIMP3 is expressed ubiquitously meaning it can be found in a variety of tissues throughout the body. As a part of the aminoacyl-tRNA synthetase (ARS) complex AIMP3 contributes to protein translation assisting in the assembly of necessary components for peptide chain formation.

Biological function summary

The role of AIMP3 extends beyond its contribution to protein synthesis. This protein participates in the macromolecular ARS complex which is essential for accurate and efficient translation of the genetic code into functional proteins. AIMP3 acts as a scaffold stabilizing the assembly and function of the ARS complex. Its involvement is critical for maintaining the fidelity of protein synthesis as errors in this process can lead to cellular dysfunction.

Pathways

AIMP3 engages in several key biological processes. It functions within the DNA damage response pathway where it interacts with proteins such as ATM and ATR to facilitate DNA repair mechanisms. Additionally AIMP3 is involved in the p53 signaling pathway where it contributes to the regulation of cell cycle and apoptosis. This interaction highlights the protein's role in maintaining genomic stability and preventing tumorigenesis.

AIMP3 has implications in cancer and neurodegenerative diseases. In cancer downregulation or mutations in AIMP3 can disrupt the DNA repair process leading to genomic instability and tumor development. The protein's interaction with p53 is particularly important as p53 mutations are common in a variety of cancers. Additionally AIMP3 has been associated with neurodegenerative disorders where improper protein synthesis may contribute to disease progression. Its relationship with these conditions highlights the broad impact of AIMP3 on human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Positive modulator of ATM response to DNA damage.

See full target information EEF1E1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5476-5495 PubMed40959275

2025

Increased CAPG inhibits ferroptosis to drive tumor proliferation and sorafenib resistance in hepatocellular carcinoma via the WDR74-p53-SLC7A11 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Quan,Fan Yao,Wenfeng Liu,Bei Tang,Miao Li,Shenxin Lu,Jinghuan Li,Rongxin Chen,Zhenggang Ren,Xin Yin

Nature cardiovascular research 4:876-890 PubMed40562875

2025

AIMP3 maintains cardiac homeostasis by regulating the editing activity of methionyl-tRNA synthetase.

Applications

Unspecified application

Species

Unspecified reactive species

Anindhya S Das,Charles P Rabolli,Colton R Martens,Han-Kai Jiang,Yingshen Zhang,Aubree A Zimmer,Kevin Lin,Kedryn K Baskin,Juan D Alfonzo,Federica Accornero

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AIMP3/p18 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (AB155689)

Western blot - Anti-AIMP3/p18 antibody (AB155689)

Western blot - Anti-AIMP3/p18 antibody (AB155689)

Western blot - Anti-AIMP3/p18 antibody (AB155689)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise