AB31543

Anti-AIMP3/p18 antibody

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(6 Publications)

Rabbit Polyclonal AIMP3/p18 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

AIMP3, P18, EEF1E1, Eukaryotic translation elongation factor 1 epsilon-1, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Elongation factor p18, Multisynthase complex auxiliary component p18

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (AB31543)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (AB31543)

Immunofluorescence analysis of Hela cells using anti-AIMP3 (p18) antibody (ab31543).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (AB31543)

Immunofluorescence analysis of U2OS cells using anti-AIMP3 (p18) antibody (ab31543).

Unknown

Western blot - Anti-AIMP3/p18 antibody (AB31543)

All lanes:

Western blot - Anti-AIMP3/p18 antibody (ab31543) at 1/20000 dilution

All lanes:

HEK293 cell line

Predicted band size: 19 kDa

Observed band size: 21 kDa

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Western blot - Anti-AIMP3/p18 antibody (AB31543)

All lanes:

Western blot - Anti-AIMP3/p18 antibody (ab31543) at 1/8000 dilution

All lanes:

whole cell lysate prepared from HEK293 cells at 50 µg/mL

Secondary

All lanes:

Goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 19 kDa

Observed band size: 20 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EEF1E1.

O43324

Specificity

ab31543 reacts specifically with 21kDa p18 and immunoprecipitates with ATM/ATR

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/8000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AIMP3 also known as p18 or eEF1A1 is a protein that plays an important role in cellular processes. It has a molecular mass of approximately 18 kDa. AIMP3 is expressed ubiquitously meaning it can be found in a variety of tissues throughout the body. As a part of the aminoacyl-tRNA synthetase (ARS) complex AIMP3 contributes to protein translation assisting in the assembly of necessary components for peptide chain formation.

Biological function summary

The role of AIMP3 extends beyond its contribution to protein synthesis. This protein participates in the macromolecular ARS complex which is essential for accurate and efficient translation of the genetic code into functional proteins. AIMP3 acts as a scaffold stabilizing the assembly and function of the ARS complex. Its involvement is critical for maintaining the fidelity of protein synthesis as errors in this process can lead to cellular dysfunction.

Pathways

AIMP3 engages in several key biological processes. It functions within the DNA damage response pathway where it interacts with proteins such as ATM and ATR to facilitate DNA repair mechanisms. Additionally AIMP3 is involved in the p53 signaling pathway where it contributes to the regulation of cell cycle and apoptosis. This interaction highlights the protein's role in maintaining genomic stability and preventing tumorigenesis.

AIMP3 has implications in cancer and neurodegenerative diseases. In cancer downregulation or mutations in AIMP3 can disrupt the DNA repair process leading to genomic instability and tumor development. The protein's interaction with p53 is particularly important as p53 mutations are common in a variety of cancers. Additionally AIMP3 has been associated with neurodegenerative disorders where improper protein synthesis may contribute to disease progression. Its relationship with these conditions highlights the broad impact of AIMP3 on human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Positive modulator of ATM response to DNA damage.

See full target information EEF1E1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Viruses 15: PubMed36851687

2023

HIV-1 Gag Binds the Multi-Aminoacyl-tRNA Synthetase Complex via the EPRS Subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Danni Jin,Yiping Zhu,Heidi L Schubert,Stephen P Goff,Karin Musier-Forsyth

Annals of translational medicine 8:1670 PubMed33490182

2021

Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Wang,Dongsheng Guan,Xu Zhao,Dongge Qiao,Yanhua Yang,Yinglin Cui

Proceedings of the National Academy of Sciences of 110:2557-62 PubMed23359686

2013

Role for the obesity-related FTO gene in the cellular sensing of amino acids.

Applications

Species

Mouse

Pawan Gulati,Man Ka Cheung,Robin Antrobus,Chris D Church,Heather P Harding,Yi-Chun Loraine Tung,Debra Rimmington,Marcella Ma,David Ron,Paul J Lehner,Frances M Ashcroft,Roger D Cox,Anthony P Coll,Stephen O'Rahilly,Giles S H Yeo

The Journal of biological chemistry 287:26302-11 PubMed22685290

2012

Elevation of highly up-regulated in liver cancer (HULC) by hepatitis B virus X protein promotes hepatoma cell proliferation via down-regulating p18.

Applications

Species

Human

Yumei Du,Guangyao Kong,Xiaona You,Shuai Zhang,Tao Zhang,Yuen Gao,Lihong Ye,Xiaodong Zhang

The Journal of biological chemistry 286:20688-700 PubMed21460219

2011

RNA binding targets aminoacyl-tRNA synthetases to translating ribosomes.

Applications

Unspecified application

Species

Human

Alexandre David,Nir Netzer,Michael Brad Strader,Suman R Das,Cai Yun Chen,James Gibbs,Philippe Pierre,Jack R Bennink,Jonathan W Yewdell

Aging cell 9:810-22 PubMed20726853

2010

Downregulation of lamin A by tumor suppressor AIMP3/p18 leads to a progeroid phenotype in mice.

Applications

Species

Mouse

Young Sun Oh,Dae Gyu Kim,Gyuyoup Kim,Eung-Chil Choi,Brian K Kennedy,Yousin Suh,Bum Joon Park,Sunghoon Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AIMP3/p18 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (AB31543)

Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (AB31543)

Western blot - Anti-AIMP3/p18 antibody (AB31543)

Western blot - Anti-AIMP3/p18 antibody (AB31543)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise