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Rabbit Recombinant Monoclonal AIP antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-AIP antibody [EPR13585] (AB192024), expandable thumbnail
  • Western blot - Anti-AIP antibody [EPR13585] (AB192024), expandable thumbnail
  • Western blot - Anti-AIP antibody [EPR13585] (AB192024), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

May play a positive role in AHR-mediated (aromatic hydrocarbon receptor) signaling, possibly by influencing its receptivity for ligand and/or its nuclear targeting. Cellular negative regulator of the hepatitis B virus (HBV) X protein.

Alternative names

XAP2, AIP, AH receptor-interacting protein, Aryl-hydrocarbon receptor-interacting protein, HBV X-associated protein 2, Immunophilin homolog ARA9, XAP-2

Recommended products

Rabbit Recombinant Monoclonal AIP antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13585
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

AIP also known as aryl hydrocarbon receptor-interacting protein is a molecular chaperone with a mass of approximately 37 kDa. It mainly resides in the cytoplasm of cells and interacts with various proteins. Expression of the AIP protein occurs in many tissues including liver lung and brain. This protein plays a significant role in maintaining the stability and proper functioning of its interacting partners.

Biological function summary

AIP acts as a modulator of protein-protein interactions impacting several signaling pathways. It is a part of the larger protein complex involving heat shock proteins which helps prevent the degradation of its client proteins. AIP is essential for the activity and stability of receptors like the aryl hydrocarbon receptor (AHR) helping these receptors remain in a functional state until they get activated.

Pathways

AIP incorporates itself into the AHR signaling pathway which affects the regulation of xenobiotic metabolism. The protein is also involved in the regulation of the glucocorticoid receptor pathway working closely with proteins like Hsp90. Through these interactions AIP influences the ability of cells to respond to environmental toxins and physiological stress thereby playing a role in cellular homeostasis.

Associated diseases and disorders

AIP has a connection with familial isolated pituitary adenomas (FIPA) and is also implicated in some forms of cancer. AIP mutations can lead to alterations in the function of the AHR receptor which is linked to these conditions. A notable interaction partner of AIP in these contexts is the pituitary tumor transforming gene (PTTG) which highlights its role in tumorigenesis and the regulation of cell proliferation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024), expandable thumbnail

    Western blot - Anti-AIP antibody [EPR13585] (ab192024)

    Lanes 1-3: Merged signal (red and green). Green - ab192024 observed at 38 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab192024 Anti-AIP antibody [EPR13585] was shown to specifically react with AIP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human AIP knockout HeLa cell line ab265502 (knockout cell lysate Human AIP knockout HeLa cell lysate ab257822) was used. Wild-type and AIP knockout samples were subjected to SDS-PAGE. ab192024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-AIP antibody [EPR13585] (ab192024) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: AIP knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human AIP knockout HeLa cell line (Human AIP knockout HeLa cell line ab265502)

    Lane 3: HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 37 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024), expandable thumbnail

    Western blot - Anti-AIP antibody [EPR13585] (ab192024)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-AIP antibody [EPR13585] (ab192024) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: Jurkat cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: Human thymus cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-AIP antibody [EPR13585] (ab192024), expandable thumbnail

    Western blot - Anti-AIP antibody [EPR13585] (ab192024)

    Blocking/dilution buffer: 5% NFDM /TBST.

    All lanes: Western blot - Anti-AIP antibody [EPR13585] (ab192024) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: Mouse spleen lysate at 10 µg

    Lane 5: Rat heart lysate at 10 µg

    Lane 6: Rat kidney lysate at 10 µg

    Lane 7: C6 cell lysate at 10 µg

    Lane 8: Raw 264.7 cell lysate at 10 µg

    Lane 9: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa

    Observed band size: 38 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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