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AB251106

Anti-AIP antibody [EPR13585] - BSA and Azide free

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Rabbit Recombinant Monoclonal AIP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

XAP2, AIP, AH receptor-interacting protein, Aryl-hydrocarbon receptor-interacting protein, HBV X-associated protein 2, Immunophilin homolog ARA9, XAP-2

1 Images
Western blot - Anti-AIP antibody [EPR13585] - BSA and Azide free (AB251106)
  • WB

Lab

Western blot - Anti-AIP antibody [EPR13585] - BSA and Azide free (AB251106)

This data was developed using the same antibody clone in a different buffer formulation (ab192024).

Lanes 1-3 : Merged signal (red and green). Green - ab192024 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

ab192024 Anti-AIP antibody [EPR13585] was shown to specifically react with AIP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265502 (knockout cell lysate ab257822) was used. Wild-type and AIP knockout samples were subjected to SDS-PAGE. ab192024 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-AIP antibody [EPR13585] (<a href='/en-us/products/primary-antibodies/aip-antibody-epr13585-ab192024'>ab192024</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

AIP knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human AIP knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-aip-knockout-hela-cell-line-ab265502'>ab265502</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 37 kDa

Observed band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13585

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251106 is the carrier-free version of ab192024.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AIP also known as aryl hydrocarbon receptor-interacting protein is a molecular chaperone with a mass of approximately 37 kDa. It mainly resides in the cytoplasm of cells and interacts with various proteins. Expression of the AIP protein occurs in many tissues including liver lung and brain. This protein plays a significant role in maintaining the stability and proper functioning of its interacting partners.
Biological function summary

AIP acts as a modulator of protein-protein interactions impacting several signaling pathways. It is a part of the larger protein complex involving heat shock proteins which helps prevent the degradation of its client proteins. AIP is essential for the activity and stability of receptors like the aryl hydrocarbon receptor (AHR) helping these receptors remain in a functional state until they get activated.

Pathways

AIP incorporates itself into the AHR signaling pathway which affects the regulation of xenobiotic metabolism. The protein is also involved in the regulation of the glucocorticoid receptor pathway working closely with proteins like Hsp90. Through these interactions AIP influences the ability of cells to respond to environmental toxins and physiological stress thereby playing a role in cellular homeostasis.

AIP has a connection with familial isolated pituitary adenomas (FIPA) and is also implicated in some forms of cancer. AIP mutations can lead to alterations in the function of the AHR receptor which is linked to these conditions. A notable interaction partner of AIP in these contexts is the pituitary tumor transforming gene (PTTG) which highlights its role in tumorigenesis and the regulation of cell proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a positive role in AHR-mediated (aromatic hydrocarbon receptor) signaling, possibly by influencing its receptivity for ligand and/or its nuclear targeting.. Cellular negative regulator of the hepatitis B virus (HBV) X protein.
See full target information AIP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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