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AB248248

Anti-AIPL1 antibody [EPR7711] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal AIPL1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AIPL2, AIPL1, Aryl-hydrocarbon-interacting protein-like 1

3 Images
Flow Cytometry (Intracellular) - Anti-AIPL1 antibody [EPR7711] - BSA and Azide free (AB248248)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-AIPL1 antibody [EPR7711] - BSA and Azide free (AB248248)

This data was developed using ab128960, the same antibody clone in a different buffer formulation.

ab128960, at 1/10 dilution staining AIPL1 (red) in permeabilized Y79 cells, compared with a negative rabbit IgG (green) by intracellular flow cytometry.

Western blot - Anti-AIPL1 antibody [EPR7711] - BSA and Azide free (AB248248)
  • WB

Unknown

Western blot - Anti-AIPL1 antibody [EPR7711] - BSA and Azide free (AB248248)

This data was developed using ab128960, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AIPL1 antibody [EPR7711] (<a href='/en-us/products/primary-antibodies/aipl1-antibody-epr7711-ab128960'>ab128960</a>) at 1/10000 dilution

All lanes:

Y79 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

OI-RD Scanning - Anti-AIPL1 antibody [EPR7711] - BSA and Azide free (AB248248)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-AIPL1 antibody [EPR7711] - BSA and Azide free (AB248248)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7711

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab248248 is the carrier-free version of ab128960.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AIPL1 also known as aryl hydrocarbon receptor-interacting protein-like 1 is a protein that plays an important mechanical role in the cellular environment. It has a molecular mass of approximately 42 kDa. AIPL1 is expressed mainly in the retina further appearing in photoreceptor cells where its function becomes important for proper cell operation. This protein interacts with other molecular structures directly affecting processes essential to cellular function.
Biological function summary

AIPL1 assists in maintaining the stability and proper folding of photoreceptor PDE6 a cyclic GMP phosphodiesterase an important component of the phototransduction complex. It acts as a chaperone ensuring that PDE6 which is critical for visual processes stays correctly assembled and functional. Its role in this complex highlights its involvement in the health of photoreceptor cells and indirectly supports the conversion of light into neural signals within the eye.

Pathways

AIPL1 participates significantly within the phototransduction pathway. This is a vital pathway required for vision where it directly influences the regulation of PDE6 activity. AIPL1's association with PDE6 links it to further elements like the guanylate cyclase pathway important for replenishing cGMP levels post-phototransduction. Interactions with proteins such as chaperonin-containing TCP-1 (CCT) highlight its role in the folding and proper functioning of phototransduction components.

Mutations in AIPL1 are associated with Leber congenital amaurosis (LCA) a severe inherited retinal dystrophy that leads to blindness or severe vision impairment from a young age. The loss of function or malfunction of AIPL1 affects PDE6 leading to the buildup of misfolded proteins which impairs photoreceptor cell survival and function. Additionally study of similar AIPL1 mutations sheds light on a subset of retinitis pigmentosa linking abnormalities within this protein to progressive vision disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be important in protein trafficking and/or protein folding and stabilization.
See full target information AIPL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20220613 PubMed36694627

2023

circ-IARS depletion inhibits the progression of non-small-cell lung cancer by circ-IARS/miR-1252-5p/HDGF ceRNA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Yang,Chunping Yang,Ping Li
View all publications
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