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AB243169

Anti-AIRE antibody [5H12]

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(1 Publication)

Rat Recombinant Monoclonal AIRE antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Autoimmune regulator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog, APECED protein homolog, Aire

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [5H12] (AB243169)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [5H12] (AB243169)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling AIRE with ab243169 at 1/500 dilution (3.428μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Nuclear staining in the medulla of mouse thymus tissue is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [5H12] (AB243169)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [5H12] (AB243169)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling AIRE with ab243169 at 1/500 dilution (3.428μg/ml) followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin.

Negative control : No staining on mouse spleen (PMID : 18322189).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Carrier free

    Anti-AIRE antibody [5H12] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

5H12

Isotype

IgG2c

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "3.428 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.428 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "3.428 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AIRE short for Autoimmune Regulator is a transcription factor with a molecular mass of about 58 kDa. It plays an important role in the immune system by promoting the expression of self-antigens in the thymus. This activity contributes to the elimination of self-reactive T cells important for preventing autoimmune responses. AIRE primarily shows expression in the medullary thymic epithelial cells (mTECs) where it ensures that immature T cells get exposed to a wide range of antigens.
Biological function summary

AIRE functions to maintain central tolerance in the immune system. It acts as a part of a transcriptional complex that regulates genes involved in immune tolerance. This complex controls the expression of tissue-specific antigens (TSAs) important for "educating" T cells in the thymus to distinguish between self and non-self antigens. By controlling this mechanism AIRE helps to lower the risk of the body attacking its own tissues.

Pathways

AIRE contributes significantly to the pathway of immune tolerance and T cell selection within the thymus. It interacts with several other proteins involved in these pathways including the tumor necrosis factor receptor superfamily member RANK influencing the maturation of mTECs. Additionally AIRE interacts with proteins like CHD4 and a range of histone-modifying enzymes to modulate gene expression more broadly within the context of epigenetic regulation.

Mutations or malfunctions in AIRE relate to autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) also known as Autoimmune Polyendocrine Syndrome Type 1 (APS-1). AIRE deficiency impairs negative selection of self-reactive T cells leading to multi-organ autoimmunity. This condition links AIRE with other proteins involved in the maintenance of immune homeostasis such as CTLA-4 which has roles in restraining immune activation and maintaining self-tolerance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA) (Probable). Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla (By similarity). Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program (PubMed : 19015306). Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression (PubMed : 21300913). Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes (PubMed : 19923453). In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection (PubMed : 26070482). In secondary lymphoid organs, induces functional inactivation of CD4(+) T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli (PubMed : 23993652).
See full target information Aire
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 180:3824-32 PubMed18322189

2008

A specific anti-Aire antibody reveals aire expression is restricted to medullary thymic epithelial cells and not expressed in periphery.

Applications

Unspecified application

Species

Unspecified reactive species

François-Xavier Hubert,Sarah A Kinkel,Kylie E Webster,Ping Cannon,Pauline E Crewther,Anna I Proeitto,Li Wu,William R Heath,Hamish S Scott
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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