Anti-AIRE antibody [EPR28045-139] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [EPR28045-139] - BSA and Azide free (AB315165)

This data was developed using ab315164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labeling AIRE with ab315164 at 1/500 (1.034 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on a few cells in the medulla of mouse thymus (PMID : 15492124; PMID : 28232470). The section was incubated with ab315164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [EPR28045-139] - BSA and Azide free (AB315165)

This data was developed using ab315164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling AIRE with ab315164 at 1/500 (1.034 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse liver (PMID : 10925280). The section was incubated with ab315164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [EPR28045-139] - BSA and Azide free (AB315165)

This data was developed using ab315164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat thymus tissue labeling AIRE with ab315164 at 1/500 (1.034 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on a few cells in the medulla of rat thymus. The section was incubated with ab315164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [EPR28045-139] - BSA and Azide free (AB315165)

This data was developed using ab315164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling AIRE with ab315164 at 1/500 (1.034 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat cerebrum. The section was incubated with ab315164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [EPR28045-139] - BSA and Azide free (AB315165)

This data was developed using ab315164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling AIRE with ab315164 at 1/500 (1.034 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse cerebrum (PMID : 10925280). The section was incubated with ab315164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIRE antibody [EPR28045-139] - BSA and Azide free (AB315165)

This data was developed using ab315164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling AIRE with ab315164 at 1/500 (1.034 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat liver. The section was incubated with ab315164 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-AIRE antibody [EPR28045-139] - BSA and Azide free (AB315165)

This data was developed using ab315164, the same antibody clone in a different buffer formulation.

AIRE was immunoprecipitated from 0.35 mg Mouse thymus tissue lysate with ab315164 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315164 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse thymus tissue lysate
Lane 2 : ab315164 IP in Mouse thymus tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315164 in Mouse thymus tissue lysate

All lanes:

Immunoprecipitation - Anti-AIRE antibody [EPR28045-139] (<a href='/en-us/products/primary-antibodies/aire-antibody-epr28045-139-ab315164'>ab315164</a>) at 1/30 dilution

All lanes:

Mouse thymus tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-AIRE antibody [EPR28045-139] - BSA and Azide free (AB315165)

This data was developed using ab315164, the same antibody clone in a different buffer formulation.

Negative control : mouse kidney (PMID : 18214467, PMID : 10925280), mouse liver (PMID : 18214467, PMID : 10925280), mouse skin (PMID : 18214467, PMID : 10925280).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-AIRE antibody [EPR28045-139] (<a href='/en-us/products/primary-antibodies/aire-antibody-epr28045-139-ab315164'>ab315164</a>) at 1/1000 dilution

Lane 1:

Mouse thymus tissue lysate at 50 µg

Lane 2:

Mouse kidney tissue lysate at 50 µg

Lane 3:

Mouse liver tissue lysate at 50 µg

Lane 4:

Mouse skin tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 59 kDa,36 kDa

false

Exposure time: 26s