AB244285

Anti-Ajuba antibody

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(3 Publications)

Knockout Tested Rabbit Polyclonal AJUBA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AJUBA aa 200-350.

View Alternative Names

JUB, AJUBA, LIM domain-containing protein ajuba

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunohistochemical staining of human skin shows moderate nuclear positivity in squamous epithelial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in tubules.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunohistochemical staining of human Fallopian tube shows strong nuclear positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-Ajuba antibody (AB244285)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ajuba antibody (AB244285)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Ajuba (green) using ab244285 at 4 μg/ml in ICC/IF.

Unknown

Western blot - Anti-Ajuba antibody (AB244285)

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Western blot - Anti-Ajuba antibody (ab244285) at 0.4 µg/mL

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HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 57 kDa

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Lab

Western blot - Anti-Ajuba antibody (AB244285)

Western blot : Anti-AJUBA antibody (ab244285) staining at 0.1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab244285 was shown to bind specifically to AJUBA. A band was observed at 55-60 kDa in wild-type A549 cell lysates with no signal observed at this size in AJUBA knockout cell line. To generate this image, wild-type and AJUBA knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

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Western blot - Anti-Ajuba antibody (ab244285) at 1 µg/mL

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

AJUBA knockout A549 cell lysate at 20 µg

Lane 3:

HCT 116 cell lysate at 20 µg

Lane 4:

MEF1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 55 kDa,60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AJUBA aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q96IF1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ajuba also known as Juba or 8 Ajuba is a LIM domain protein with a molecular mass of around 55 kDa. This protein shows expression in various tissues including the skin and immune cells. Mechanically Ajuba functions as a scaffold protein interacting with several other proteins to regulate cellular processes. It helps in the assembly and stabilization of protein complexes especially at the cell surface and in the nucleus playing an important role in connecting cytoskeletal components to signaling pathways.

Biological function summary

Ajuba influences cell proliferation adhesion and migration. As part of the Ajuba-Zyxin-LIMD1 complex it interacts with actin filaments and diverse cytoskeletal proteins mediating signal transduction from extracellular cues to the nucleus. Through these interactions Ajuba modulates gene expression essential for cell cycle progression and differentiation emphasizing its significance in development and tissue maintenance.

Pathways

Ajuba participates in the Wnt signaling and the Hippo pathway. Within Wnt signaling Ajuba partners with key proteins like β-catenin to regulate transcriptional activity and cell fate determination. Additionally in the Hippo pathway Ajuba associates with large tumor suppressor kinases (LATS) and YAP influencing cellular growth and apoptosis. These pathways demonstrate Ajuba's involvement in maintaining cellular homoeostasis and response to environmental stimuli.

Ajuba's dysregulation associates with cancer and autoimmune diseases. In cancer altered Ajuba expression affects cell survival and proliferation tying it to proteins such as β-catenin in colorectal cancer progression. In autoimmune diseases improper Ajuba functioning disrupts immune cell signaling and tolerance impacting proteins like NF-kB. These connections highlight Ajuba's role in pathological conditions and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.

See full target information AJUBA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 21:44 PubMed39737130

2024

Targeting the Ajuba/Notch axis increases the sensitivity of colon cancer cells to 5-fluorouracil.

Applications

Unspecified application

Species

Unspecified reactive species

Xinghua Liang,Xuelian Liu,Long Zhang,Junhao Liu,Rong Yan,Haiyan Li,Xiancheng Zeng,Hong Wang

Development (Cambridge, England) 149: PubMed35020896

2022

Cell trajectory modeling identifies a primitive trophoblast state defined by BCAM enrichment.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Shannon,Jennet Baltayeva,Barbara Castellana,Jasmin Wächter,Gina L McNeill,Ji Soo Yoon,Jenna Treissman,Hoa T Le,Pascal M Lavoie,Alexander G Beristain

Journal of cellular and molecular medicine 24:9908-9924 PubMed32681581

2020

Hsa_circ_0128846 promotes tumorigenesis of colorectal cancer by sponging hsa-miR-1184 and releasing AJUBA and inactivating Hippo/YAP signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Wang,Yujia Chen,Wei Liu,Tao Liu,Di Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ajuba antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ajuba antibody (AB244285)

Immunocytochemistry/ Immunofluorescence - Anti-Ajuba antibody (AB244285)

Western blot - Anti-Ajuba antibody (AB244285)

Western blot - Anti-Ajuba antibody (AB244285)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise