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AB284814

Anti-AK 1 antibody [EP7083] - BSA and Azide free

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Rabbit Monoclonal AK 1 antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

View Alternative Names

Adenylate kinase isoenzyme 1, AK 1, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase, AK1

2 Images
Western blot - Anti-AK 1 antibody [EP7083] - BSA and Azide free (AB284814)
  • WB

Unknown

Western blot - Anti-AK 1 antibody [EP7083] - BSA and Azide free (AB284814)

This data was developed using ab138504, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AK 1 antibody [EP7083] (<a href='/en-us/products/primary-antibodies/ak-1-antibody-ep7083-ab138504'>ab138504</a>) at 1/1000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

U87-MG lysate at 10 µg

Lane 3:

Saos2 lysate at 10 µg

Lane 4:

HT-29 lysate at 10 µg

Lane 5:

fetal muscle lysate at 10 µg

Lane 6:

fetal heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

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Western blot - Anti-AK 1 antibody [EP7083] - BSA and Azide free (AB284814)
  • WB

Unknown

Western blot - Anti-AK 1 antibody [EP7083] - BSA and Azide free (AB284814)

This data was developed using ab138504, the same antibody clone in a different buffer formulation.

Lanes 1-3 : Merged signal (red and green). Green - ab138504 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa.

ab138504 Anti-AK 1 antibody [EP7083] was shown to specifically react with AK 1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265132 (knockout cell lysate ab257824) was used. Wild-type and AK 1 knockout samples were subjected to SDS-PAGE. ab138504 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-AK 1 antibody [EP7083] (<a href='/en-us/products/primary-antibodies/ak-1-antibody-ep7083-ab138504'>ab138504</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

AK1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human AK1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ak1-knockout-hela-cell-line-ab265132'>ab265132</a>)

Lane 3:

U-87MG cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7083

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284814 is the carrier-free version of ab138504

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenylate kinase 1 also known as AK1 plays a critical role in cellular energy homeostasis. It catalyzes the reversible transfer of a phosphate group from ATP to AMP forming two ADP molecules. AK1 is a 24-kilodalton protein largely expressed in tissues with high-energy demands like brain heart and skeletal muscle. Expression levels vary but AK1's presence is notably higher in these energy-intense regions highlighting its importance in energy transfer and equilibrium.
Biological function summary

Adenylate kinase 1 is essential for maintaining the adenine nucleotide pool balance. It does not act alone but functions within diverse cellular contexts ensuring efficient energy supply which is necessary for critical cellular processes. In these contexts AK1 forms part of a larger network that involves other kinases and molecules operating to sustain sufficient energy levels during periods of high activity or stress. This orchestration of molecules ensures cells adapt to changing energy requirements swiftly.

Pathways

AK1 is a critical component of the ATP-generating phosphotransfer network and is involved in the cellular adenylate kinase reaction pathway. It interacts closely with proteins such as AMP-activated protein kinase (AMPK) which assists in detecting and responding to energy deficits. In the context of the phosphotransfer network maintaining the balance between ATP ADP and AMP is important and AK1 enables this dynamic equilibrium. By interrelating with these pathways AK1 supports efficient energy transduction and cellular adaptation to metabolic changes.

Abnormal AK1 function or expression has been implicated in metabolic conditions like mitochondrial myopathy and heart diseases such as myocardial infarction. A disruption in the balance of adenylate kinase activity can lead to energy deficiency aggravating these conditions. Connections to these diseases involve proteins such as creatine kinase which plays a role in energy buffering. The interplay between AK1 and associated kinases is key in understanding how energetic imbalances contribute to the development and progression of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity) (PubMed : 21080915, PubMed : 23416111, PubMed : 2542324). Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP (By similarity).
See full target information AK1
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Cell Lines & Lysates

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Advanced Validation

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Product promise

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