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Rabbit Recombinant Monoclonal AK2 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

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Images

Western blot - Anti-AK2 antibody [EPR11388(B)] (AB166901), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (AB166901), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] (AB166901), expandable thumbnail
  • Immunoprecipitation - Anti-AK2 antibody [EPR11388(B)] (AB166901), expandable thumbnail
  • Western blot - Anti-AK2 antibody [EPR11388(B)] (AB166901), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Predicted
Predicted
Not recommended
Expected
Predicted
Rat
Predicted
Predicted
Not recommended
Expected
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

For unpurified use at 1/100 - 1/250.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.

Alternative names

ADK2, AK2, AK 2, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase

Recommended products

Rabbit Recombinant Monoclonal AK2 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR11388(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The adenylate kinase 2 also known as AK2 is an enzyme that catalyzes the conversion of adenine nucleotides within the cell. It facilitates the reversible conversion of ADP into ATP and AMP playing a critical role in cellular energy homeostasis. AK2 weighing approximately 26 kDa is primarily found in the mitochondrial intermembrane space of various tissues including liver muscle and brain. Its expression allows for efficient energy transfer essential for normal mitochondrial function and cellular metabolism.

Biological function summary

AK2 functions in energy transfer and regulation in the cell serving as an important component in maintaining cellular ATP levels. AK2 is an integral part of the mitochondrial adenylate kinase system which manages energy flux in cells. By balancing the concentrations of ATP ADP and AMP AK2 directly influences cellular energy state and metabolic activity. The enzyme also supports nucleotide homeostasis therefore facilitating processes such as signal transduction and nucleic acid synthesis.

Pathways

AK2 is involved in the adenylate kinase pathway important for energy metabolism. It coordinates with other enzymes like AK1 and AK3 ensuring efficient energy distribution in the cell. AK2 also interfaces with the apoptosis signaling pathways where it plays a part in regulating programmed cell death. The interaction of AK2 within these pathways emphasizes its essential role in maintaining cellular energy balance and undergoing apoptosis when necessary.

Associated diseases and disorders

AK2 mutations link to rare conditions like reticular dysgenesis a severe form of combined immunodeficiency. The disorder arises when AK2 fails to initiate proper energy transfer necessary for white blood cell development. AK2 is also related to mitochondrial disorders where energy production gets critically impaired manifesting in muscle weakness and neurodegenerative symptoms. The enzyme's connection to proteins involved in energy pathways suggests its potential contribution to the progression of such diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901), expandable thumbnail

    Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    ** Lanes 1 - 4:** Merged signal (red and green). Green - ab166901 observed at 26 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
    ab166901 was shown to react with AK2 in wild-type HEK-293T cells in western blot with loss of signal observed in AK2 knockout cell line Human AK2 knockout HEK-293T cell line ab266539 (AK2 knockout cell lysate Human AK2 knockout HEK-293T cell lysate ab257825). Wild-type and AK2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab166901 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: AK2 knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human AK2 knockout HEK-293T cell line (Human AK2 knockout HEK-293T cell line ab266539)

    Lane 3: HL-60 cell lysate at 20 µg

    Lane 4: HepG2 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AK2 using ab166901 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] (ab166901), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunocytochemistry/Immunofluorescence analysis MCF-7 (human breast carcinoma) cells labelling AK2 with purified ab166901 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Immunoprecipitation - Anti-AK2 antibody [EPR11388(B)] (ab166901), expandable thumbnail

    Immunoprecipitation - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunoprecipitation of AK2 from HeLa cell lysate pellet using ab166901 at 1/10 dilution followed by immunoblotting. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Predicted band size: 26 kDa

  • Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901), expandable thumbnail

    Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    All lanes: Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901) at 1/1000 dilution

    Lane 1: Fetal kidney lysate at 10 µg

    Lane 2: Fetal liver lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling AK2 using ab166901 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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