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AB232888

Anti-AK3 antibody

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(2 Publications)

Rabbit Polyclonal AK3 antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AK3 aa 1-250.

View Alternative Names

AK3L1, AK6, AKL3L, AK3, Adenylate kinase 3, Adenylate kinase 3 alpha-like 1, Adenylate kinase isozyme 3

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3 antibody (AB232888)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3 antibody (AB232888)

Formalin-fixed, paraffin-embedded human kidney tissue stained for AK3 with ab232888 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3 antibody (AB232888)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3 antibody (AB232888)

Formalin-fixed, paraffin-embedded human liver tissue stained for AK3 with ab232888 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3 antibody (AB232888)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3 antibody (AB232888)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for AK3 with ab232888 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3 antibody (AB232888)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3 antibody (AB232888)

Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for AK3 with ab232888 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Western blot - Anti-AK3 antibody (AB232888)
  • WB

Supplier Data

Western blot - Anti-AK3 antibody (AB232888)

All lanes:

Western blot - Anti-AK3 antibody (ab232888) at 2 µg/mL

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

Lane 2:

Pig heart tissue lysate

Lane 3:

Pig kidney tissue lysate

Lane 4:

Pig liver tissue lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/1000 dilution

Predicted band size: 26 kDa

false

Western blot - Anti-AK3 antibody (AB232888)
  • WB

Supplier Data

Western blot - Anti-AK3 antibody (AB232888)

All lanes:

Western blot - Anti-AK3 antibody (ab232888) at 2 µg/mL

All lanes:

Recombinant human AK3 protein

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AK3 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UIJ7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AK3 also known as adenylate kinase 3 is a mitochondrial enzyme involved in energy metabolism. It has a molecular mass of approximately 26 kDa and is mainly found in the mitochondrial matrix. AK3 catalyzes the reversible transfer of phosphate groups among adenosine phosphates which plays a role in cellular energy homeostasis. Expression of AK3 occurs in tissues with high metabolic rates such as the heart liver and muscle reflecting its importance in energy demand areas.
Biological function summary

Adenylate kinase 3 facilitates the maintenance of cellular energy balance by ensuring the proper concentration of adenine nucleotides within the mitochondria. This enzyme is an important component of the adenine nucleotide translocase complex which regulates nucleotide transport across the inner mitochondrial membrane. Through its activity AK3 contributes to the recycling of ATP and ADP supporting overall energy metabolism and mitochondrial function.

Pathways

Adenylate kinase 3 plays a significant role in the purine nucleotide cycle and oxidative phosphorylation pathways. In the purine nucleotide cycle it interacts with enzymes like nucleoside diphosphate kinase for maintaining proper levels of ADP and ATP. Within oxidative phosphorylation AK3 helps in balancing energy supply in concert with other mitochondrial proteins like ATP synthase ensuring efficient ATP production and utilization in energy-intensive processes.

Alterations in adenylate kinase 3 function have been linked to mitochondrial diseases and cardiac disorders. Mitochondrial dysfunction resulting from impaired AK3 activity can lead to energy deficiency contributing to diseases like Leigh syndrome. Similarly inadequate AK3 function may affect cardiac energy metabolism relating to heart failure conditions where proper energy supply is essential. AK3's interactions with proteins like creatine kinase further emphasize its role in maintaining cardiac energy stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial adenylate kinase with a specific GTP : AMP phosphotransferase activity (PubMed : 11485571, PubMed : 32822537). Could also use ITP as phosphate donor (PubMed : 11485571). Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable).
See full target information AK3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Brain communications 5:fcad001 PubMed36687391

2023

Translational potential of synaptic alterations in Alzheimer's disease patients and amyloid precursor protein knock-in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dina Medina-Vera,Daniela Enache,Simone Tambaro,Ethar Abuhashish,Cristina Rosell-Valle,Bengt Winblad,Fernando Rodríguez de Fonseca,Erika Bereczki,Per Nilsson

Cancer cell international 20:244 PubMed32549791

2020

Knockdown of LINC00662 represses AK4 and attenuates radioresistance of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangzong Chen,Chunchun Bao,Xiuxing Zhang,Xinshi Lin,Yimou Fu
View all publications
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Product promise

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