Skip to main content

Rabbit Recombinant Monoclonal AK3L1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

Be the first to review this product! Submit a review

Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Not recommended
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

-

Target data

Function

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates (PubMed:19073142, PubMed:19766732, PubMed:23416111, PubMed:24767988). Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor (PubMed:19073142, PubMed:19766732, PubMed:23416111). Also displays broad nucleoside diphosphate kinase activity (PubMed:19073142, PubMed:19766732, PubMed:23416111). Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK (PubMed:24767988, PubMed:26980435). Plays a protective role in the cellular response to oxidative stress (PubMed:19130895, PubMed:23474458, PubMed:26980435).

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Recombinant Monoclonal AK3L1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR7678

Purification technique

Affinity purification Protein A

Dissociation constant

3.23 x 10-11 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

AK3L1 regulates mitochondrial energy metabolism and supports cellular functions. It operates as part of a network managing the mitochondrial adenylate kinase isoform family participating in mitochondrial phosphotransfer systems. AK3L1 contributes to sustaining energy balance especially in tissues with high-energy demand like muscle and brain. Within these systems AK3L1 interacts with other adenylate kinases and mitochondrial proteins to balance nucleotide ratios and prevent energy deficits.

Activity summary

AK3L1 also known as adenylate kinase 3-like 1 is an enzyme with a molecular mass of approximately 26 kDa. This protein primarily catalyzes the reversible transfer of phosphate groups between ATP and AMP. AK3L1 is expressed mainly in the mitochondrial matrix playing a fundamental role in nucleotide metabolism. By facilitating energy transfer within the mitochondria AK3L1 ensures the availability of ATP and maintains cellular energy homeostasis.

Pathways

AK3L1 integrates into the wider cellular energy regulation and oxidative phosphorylation pathways. It supports the cellular energy landscape by linking to the citric acid cycle and oxidative phosphorylation essential for ATP production. AK3L1 coordinates with proteins like AK2 influencing energy metabolism and ensuring effective cellular processes. By aligning with these pathways AK3L1 plays a significant role in ensuring efficient bioenergetic processes.

Associated diseases and disorders

AK3L1 features involvement in conditions such as mitochondrial myopathy and neurodegenerative disorders. Its dysfunction can disrupt mitochondrial energy balance linking it to diseases characterized by energy production deficits. The protein closely interacts with AK2 and other mitochondrial enzymes whose imbalance can further contribute to disease development. Understanding AK3L1's role provides insight into potential therapeutic targets for these disorders addressing the underlying mitochondrial dysfunctions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-AK3L1 antibody [EPR7678] (ab131327), expandable thumbnail

    Western blot - Anti-AK3L1 antibody [EPR7678] (ab131327)

    Lanes 1-4: Merged signal (red and green). Green - ab131327 observed at 25 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab131327 Anti-AK3L1 antibody [EPR7678] was shown to specifically react with Adenylate kinase 4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265331 (knockout cell lysate ab257827) was used. Wild-type and Adenylate kinase 4 knockout samples were subjected to SDS-PAGE. ab131327 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-AK3L1 antibody [EPR7678] (AB131327) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: AK3L1 knockout HeLa cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (AB216773) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 25 kDa

    Observed band size: 25 kDa

    This data was developed using the same antibody clone in a different buffer formulation (ab131327).

    Lanes 1-4: Merged signal (red and green). Green - ab131327 observed at 25 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab131327 Anti-AK3L1 antibody [EPR7678] was shown to specifically react with Adenylate kinase 4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265331 (knockout cell lysate ab257827) was used. Wild-type and Adenylate kinase 4 knockout samples were subjected to SDS-PAGE. ab131327 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3L1 antibody [EPR7678] (ab131327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3L1 antibody [EPR7678] (ab131327)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling AK3L1 with ab131327 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-AK3L1 antibody [EPR7678] (ab131327), expandable thumbnail

    Western blot - Anti-AK3L1 antibody [EPR7678] (ab131327)

    All lanes: Western blot - Anti-AK3L1 antibody [EPR7678] (AB131327) at 1/1000 dilution

    Lane 1: MCF-7 cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: Raji cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AK3L1 antibody [EPR7678] (ab131327), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AK3L1 antibody [EPR7678] (ab131327)

    Immunofluorescent analysis of HepG2 cells labelling AK3L1 with ab131327 at 1/250 dilution.

  • OI-RD Scanning - Anti-AK3L1 antibody [EPR7678] (ab131327), expandable thumbnail

    OI-RD Scanning - Anti-AK3L1 antibody [EPR7678] (ab131327)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com