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AB131327

Anti-AK3L1 antibody [EPR7678]

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(4 Publications)

Rabbit Recombinant Monoclonal AK3L1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

AK3, AK3L1, AK4, Adenylate kinase 3-like, GTP:AMP phosphotransferase AK4

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3L1 antibody [EPR7678] (AB131327)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK3L1 antibody [EPR7678] (AB131327)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling AK3L1 with ab131327 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-AK3L1 antibody [EPR7678] (AB131327)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AK3L1 antibody [EPR7678] (AB131327)

Immunofluorescent analysis of HepG2 cells labelling AK3L1 with ab131327 at 1/250 dilution.

Western blot - Anti-AK3L1 antibody [EPR7678] (AB131327)
  • WB

Lab

Western blot - Anti-AK3L1 antibody [EPR7678] (AB131327)

Lanes 1-4 : Merged signal (red and green). Green - ab131327 observed at 25 kDa. Red - loading control ab8245 observed at 37 kDa.

ab131327 Anti-AK3L1 antibody [EPR7678] was shown to specifically react with Adenylate kinase 4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265331 (knockout cell lysate ab257827) was used. Wild-type and Adenylate kinase 4 knockout samples were subjected to SDS-PAGE. ab131327 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-AK3L1 antibody [EPR7678] (ab131327) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

AK3L1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human AK4 (AK3L1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ak4-ak3l1-knockout-hela-cell-line-ab265331'>ab265331</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

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Western blot - Anti-AK3L1 antibody [EPR7678] (AB131327)
  • WB

Unknown

Western blot - Anti-AK3L1 antibody [EPR7678] (AB131327)

All lanes:

Western blot - Anti-AK3L1 antibody [EPR7678] (ab131327) at 1/1000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 25 kDa

false

OI-RD Scanning - Anti-AK3L1 antibody [EPR7678] (AB131327)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-AK3L1 antibody [EPR7678] (AB131327)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7678

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AK3L1 also known as adenylate kinase 3-like 1 is an enzyme with a molecular mass of approximately 26 kDa. This protein primarily catalyzes the reversible transfer of phosphate groups between ATP and AMP. AK3L1 is expressed mainly in the mitochondrial matrix playing a fundamental role in nucleotide metabolism. By facilitating energy transfer within the mitochondria AK3L1 ensures the availability of ATP and maintains cellular energy homeostasis.
Biological function summary

AK3L1 regulates mitochondrial energy metabolism and supports cellular functions. It operates as part of a network managing the mitochondrial adenylate kinase isoform family participating in mitochondrial phosphotransfer systems. AK3L1 contributes to sustaining energy balance especially in tissues with high-energy demand like muscle and brain. Within these systems AK3L1 interacts with other adenylate kinases and mitochondrial proteins to balance nucleotide ratios and prevent energy deficits.

Pathways

AK3L1 integrates into the wider cellular energy regulation and oxidative phosphorylation pathways. It supports the cellular energy landscape by linking to the citric acid cycle and oxidative phosphorylation essential for ATP production. AK3L1 coordinates with proteins like AK2 influencing energy metabolism and ensuring effective cellular processes. By aligning with these pathways AK3L1 plays a significant role in ensuring efficient bioenergetic processes.

AK3L1 features involvement in conditions such as mitochondrial myopathy and neurodegenerative disorders. Its dysfunction can disrupt mitochondrial energy balance linking it to diseases characterized by energy production deficits. The protein closely interacts with AK2 and other mitochondrial enzymes whose imbalance can further contribute to disease development. Understanding AK3L1's role provides insight into potential therapeutic targets for these disorders addressing the underlying mitochondrial dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Broad-specificity mitochondrial nucleoside phosphate kinase involved in cellular nucleotide homeostasis by catalyzing nucleoside-phosphate interconversions (PubMed : 19073142, PubMed : 19766732, PubMed : 23416111, PubMed : 24767988). Similar to other adenylate kinases, preferentially catalyzes the phosphorylation of the nucleoside monophosphate AMP with ATP as phosphate donor to produce ADP (PubMed : 19766732). Phosphorylates only AMP when using GTP as phosphate donor (PubMed : 19766732). In vitro, can also catalyze the phosphorylation of CMP, dAMP and dCMP and use GTP as an alternate phosphate donor (PubMed : 19766732, PubMed : 23416111). Moreover, exhibits a diphosphate kinase activity, producing ATP, CTP, GTP, UTP, TTP, dATP, dCTP and dGTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donors (PubMed : 23416111). Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK (PubMed : 24767988, PubMed : 26980435). Plays a protective role in the cellular response to oxidative stress (PubMed : 19130895, PubMed : 23474458, PubMed : 26980435).
See full target information AK4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 144:2722-2731 PubMed34581780

2021

MLIP causes recessive myopathy with rhabdomyolysis, myalgia and baseline elevated  serum creatine kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Osorio Lopes Abath Neto,Livija Medne,Sandra Donkervoort,Maria Elena Rodríguez-García,Véronique Bolduc,Ying Hu,Eleonora Guadagnin,A Reghan Foley,John F Brandsema,Allan M Glanzman,Gihan I Tennekoon,Mariarita Santi,Justin H Berger,Lynn A Megeney,Hirofumi Komaki,Michio Inoue,Francisco Javier Cotrina-Vinagre,Aurelio Hernández-Lain,Elena Martin-Hernández,Linford Williams,Sabine Borell,David Schorling,Kimberly Lin,Konstantinos Kolokotronis,Uta Lichter-Konecki,Janbernd Kirschner,Ichizo Nishino,Brenda Banwell,Francisco Martínez-Azorín,Patrick G Burgon,Carsten G Bönnemann

Oncology letters 20:346 PubMed33123257

2020

Identification of AK4 as a novel therapeutic target for serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Minmin Huang,Xinlei Qin,Yuwei Wang,Furong Mao

Disease markers 2019:8186091 PubMed31827645

2019

AK4 Promotes the Progression of HER2-Positive Breast Cancer by Facilitating Cell Proliferation and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Yan-Tao Yin,Chi-Hua Wu,Rong-Lin Qiu,Wen-Jun Jiang,Xiao-Geng Deng,Zhi-Xi Li

Clinical and experimental medicine 19:525-534 PubMed31463832

2019

Adenylate kinase 4 promotes bladder cancer cell proliferation and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Xin,Dong-Wei Yao,Li Fan,Jiu-Hua Liu,Xiao-Dong Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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