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Rabbit Recombinant Monoclonal AK3L1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.

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Images

Western blot - Anti-AK3L1 antibody [EPR7678] - BSA and Azide free (AB248407), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Not recommended
Tested
Expected
Expected

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Broad-specificity mitochondrial nucleoside phosphate kinase involved in cellular nucleotide homeostasis by catalyzing nucleoside-phosphate interconversions (PubMed:19073142, PubMed:19766732, PubMed:23416111, PubMed:24767988). Similar to other adenylate kinases, preferentially catalyzes the phosphorylation of the nucleoside monophosphate AMP with ATP as phosphate donor to produce ADP (PubMed:19766732). Phosphorylates only AMP when using GTP as phosphate donor (PubMed:19766732). In vitro, can also catalyze the phosphorylation of CMP, dAMP and dCMP and use GTP as an alternate phosphate donor (PubMed:19766732, PubMed:23416111). Moreover, exhibits a diphosphate kinase activity, producing ATP, CTP, GTP, UTP, TTP, dATP, dCTP and dGTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donors (PubMed:23416111). Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK (PubMed:24767988, PubMed:26980435). Plays a protective role in the cellular response to oxidative stress (PubMed:19130895, PubMed:23474458, PubMed:26980435).

Alternative names

AK3, AK3L1, AK4, Adenylate kinase 3-like, GTP:AMP phosphotransferase AK4

Recommended products

Rabbit Recombinant Monoclonal AK3L1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7678
Purification technique
Affinity purification Protein A
Dissociation constant
3.23 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248407 is the carrier-free version of Anti-AK3L1 antibody [EPR7678] ab131327.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

AK3L1 also known as adenylate kinase 3-like 1 is an enzyme with a molecular mass of approximately 26 kDa. This protein primarily catalyzes the reversible transfer of phosphate groups between ATP and AMP. AK3L1 is expressed mainly in the mitochondrial matrix playing a fundamental role in nucleotide metabolism. By facilitating energy transfer within the mitochondria AK3L1 ensures the availability of ATP and maintains cellular energy homeostasis.

Biological function summary

AK3L1 regulates mitochondrial energy metabolism and supports cellular functions. It operates as part of a network managing the mitochondrial adenylate kinase isoform family participating in mitochondrial phosphotransfer systems. AK3L1 contributes to sustaining energy balance especially in tissues with high-energy demand like muscle and brain. Within these systems AK3L1 interacts with other adenylate kinases and mitochondrial proteins to balance nucleotide ratios and prevent energy deficits.

Pathways

AK3L1 integrates into the wider cellular energy regulation and oxidative phosphorylation pathways. It supports the cellular energy landscape by linking to the citric acid cycle and oxidative phosphorylation essential for ATP production. AK3L1 coordinates with proteins like AK2 influencing energy metabolism and ensuring effective cellular processes. By aligning with these pathways AK3L1 plays a significant role in ensuring efficient bioenergetic processes.

Associated diseases and disorders

AK3L1 features involvement in conditions such as mitochondrial myopathy and neurodegenerative disorders. Its dysfunction can disrupt mitochondrial energy balance linking it to diseases characterized by energy production deficits. The protein closely interacts with AK2 and other mitochondrial enzymes whose imbalance can further contribute to disease development. Understanding AK3L1's role provides insight into potential therapeutic targets for these disorders addressing the underlying mitochondrial dysfunctions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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