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AB117927

Anti-AK5 antibody [OTI1D6]

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(1 Publication)

Mouse Monoclonal AK5 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Adenylate kinase isoenzyme 5.

View Alternative Names

Adenylate kinase isoenzyme 5, AK 5, ATP-AMP transphosphorylase 5, AK5

3 Images
Flow Cytometry - Anti-AK5 antibody [OTI1D6] (AB117927)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-AK5 antibody [OTI1D6] (AB117927)

Flow Cytometry analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1, using ab117927 (red) compared to an empty vector control plasmid (blue).

Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (AB117927)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (AB117927)

pCMV6-ENTRY AK5 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for AK5 using ab117927 at a 1/100 dilution in ICC/IF.

Western blot - Anti-AK5 antibody [OTI1D6] (AB117927)
  • WB

Unknown

Western blot - Anti-AK5 antibody [OTI1D6] (AB117927)

All lanes:

Western blot - Anti-AK5 antibody [OTI1D6] (ab117927) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY AK5 cDNA at 5 µg

Predicted band size: 63 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1D6

Isotype

IgG1

Carrier free

No

Reacts with

Human, African green monkey

Applications

Flow Cyt, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Adenylate kinase isoenzyme 5.

Q9Y6K8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Clone OTI1D6 (formerly 1D6).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AK5 or Adenylate Kinase 5 is an enzyme with a specialized role in phosphate group transfer. It functions by catalyzing the reversible transfer of phosphate groups from ATP to AMP which results in the production of ADP. The enzyme has a molecular mass of approximately 26 kDa. AK5 typically shows expression in the brain where it can significantly influence energy transfer and cellular energy balance.
Biological function summary

The function of AK5 involves maintaining energy homeostasis in neural cells. It does not act independently; rather it functions as a part of a larger complex that regulates cellular energetic states. In neural tissues AK5 may have substantial impacts on cellular activity by controlling the levels of key adenylates therefore influencing intracellular signaling pathways.

Pathways

AK5 plays a part in the cellular energy homeostasis pathway and is integral to the purine metabolism pathway. In these pathways AK5 interacts with other adenylate kinases and has associations with enzymes such as AMP-activated protein kinase (AMPK) which acts as an energy sensor. AK5's role in these pathways helps to maintain the balance of adenine nucleotides critical for cellular functions.

Abnormalities in AK5 expression or function can be associated with neurological conditions such as epilepsy. AK5's connection with epilepsy relates partly through its involvement in maintaining proper cellular energetic conditions critical for normal neural activity. It may also relate to metabolic disorders because changes in adenylate kinase function can impact metabolic regulation in the brain. AK5's relationship with AMPK hints at how it might link to these conditions given AMPK's role in detecting cellular energy levels.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.
See full target information Adenylate kinase isoenzyme 5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 18:1551-1559 PubMed29901122

2018

Proteomic profiling for plasma biomarkers of tuberculosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyue Liu,Liping Pan,Fen Han,Baojian Luo,Hongyan Jia,Aiying Xing,Qi Li,Zongde Zhang
View all publications
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