AB248732

Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal AKAP 95 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AKAP95, AKAP8, A-kinase anchor protein 8, AKAP-8, A-kinase anchor protein 95 kDa, AKAP 95

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

This data was developed using ab134923, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab134923 showing positive staining in Normal heart tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

This data was developed using ab134923, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab134923 showing positive staining in Normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

This data was developed using ab134923, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab134923 showing positive staining in Normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

This data was developed using ab134923, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil staining AKAP 95 with ab134923 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

This data was developed using ab134923, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue staining AKAP 95 with ab134923 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

This data was developed using ab134923, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HepG2 cells staining AKAP 95 with ab134923 at a 1/100 dilution.

Unknown

Western blot - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

This data was developed using ab134923, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AKAP 95 antibody [EPR8978(B)] (<a href='/en-us/products/primary-antibodies/akap-95-antibody-epr8978b-ab134923'>ab134923</a>) at 1/1000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

BxPC3 lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Lane 5:

HeLa lysate at 10 µg

Lane 6:

Fetal liver lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 76 kDa

false

OI-RD Scanning - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-AKAP 95 antibody [EPR8978(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8978(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248732 is the carrier-free version of ab134923.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKAP95 also known as A-kinase anchor protein 95 is a scaffolding protein with a mass of approximately 85-100 kDa. This protein facilitates the localization of protein kinase A (PKA) to specific subcellular compartments optimizing its function. AKAP95 is expressed in the nucleus of various cell types where it participates in organizing and assembling multi-protein complexes. It plays a mechanical role in linking PKA to specific substrates and other signaling molecules which influences cellular processes.

Biological function summary

AKAP95 integrates into protein complexes that regulate key aspects of cell cycle progression and transcription. It acts in chromatin remodeling which influences gene expression. AKAP95 interacts with several regulatory proteins such as cyclin-dependent kinases to affect cell division. Its role extends to ensuring efficient mitotic entry and chromosome segregation. Through these interactions AKAP95 serves as a central node in the orchestration of nuclear events critical for cell proliferation.

Pathways

AKAP95 operates within signaling networks like the cAMP/PKA pathway and the cell cycle regulation pathway. Through these pathways it connects intimately with proteins such as PKA and cyclin-dependent kinases. AKAP95 binds to these proteins facilitating the orchestration of phosphorylation events that drive cellular responses to external and internal stimuli. These pathways highlight AKAP95’s connectivity in transducing signals necessary for maintaining cellular homeostasis and proliferation control.

AKAP95 shows connections to cancer and cardiac disease. Alterations in its expression and function associate with oncogenic processes involving abnormal cell proliferation commonly seen in cancers. Interactions with proteins like cyclin-dependent kinases highlight its role in tumorigenesis. Additionally dysregulated cAMP/PKA signaling linked to AKAP95 contributes to cardiac dysfunction. Therefore studying AKAP95 in these contexts provides insight into its potential as a biomarker or therapeutic target in these pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed : 9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed : 10601332, PubMed : 10791967, PubMed : 11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed : 14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L (PubMed : 16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed : 22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed : 12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation (PubMed : 23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed : 16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed : 26880274). May play a repressive role in the regulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro. In cells, associates with ribosomal RNA (rRNA) chromatin, preferentially with rRNA promoter and transcribed regions (PubMed : 26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity).

See full target information AKAP8

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4467 PubMed37491377

2023

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhong,Xujia Wu,Yixin Gao,Junju Chen,Maolei Zhang,Huangkai Zhou,Jia Yang,Feizhe Xiao,Xuesong Yang,Nunu Huang,Haoyue Qi,Xiuxing Wang,Fan Bai,Yu Shi,Nu Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

Immunocytochemistry/ Immunofluorescence - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

Western blot - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

OI-RD Scanning - Anti-AKAP 95 antibody [EPR8978(B)] - BSA and Azide free (AB248732)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise