AB249759

Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal AKAP associated Sperm Protein/ROPN1L antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ASP, RSPH11, ROPN1L, Ropporin-1-like protein, ROPN1-like protein, AKAP-associated sperm protein

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

This data was developed using ab173298, the same antibody clone in a different buffer formulation.

ab173298 showing -ve staining in Human skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

This data was developed using ab173298, the same antibody clone in a different buffer formulation.

ab173298 showing +ve staining in Human normal testis tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

This data was developed using ab173298, the same antibody clone in a different buffer formulation.

ab173298 showing -ve staining in Human cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

This data was developed using ab173298, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling AKAP associated Sperm Protein/ROPN1L with ab173298 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

This data was developed using ab173298, the same antibody clone in a different buffer formulation.

ab173298 showing -ve staining in Human breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

This data was developed using ab173298, the same antibody clone in a different buffer formulation.

ab173298 showing -ve staining in Human normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

This data was developed using ab173298, the same antibody clone in a different buffer formulation.

Immunoprecipitation. ab173298 at 1/1000 staining AKAP associated Sperm Protein/ROPN1L immunoprecipitated from Human tetis lysate using ab173297 at 1/10.

Lane 2 : 1X PBS (negative control).

All lanes:

Immunoprecipitation - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (<a href='/en-us/products/primary-antibodies/akap-associated-sperm-protein-ropn1l-antibody-epr11266b-ab173298'>ab173298</a>)

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Supplier Data

Western blot - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

This data was developed using ab173298, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (<a href='/en-us/products/primary-antibodies/akap-associated-sperm-protein-ropn1l-antibody-epr11266b-ab173298'>ab173298</a>) at 1/1000 dilution

All lanes:

Human testis lysate at 10 µg

Predicted band size: 26 kDa

false

Unconjugated

Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11266(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249759 is the carrier-free version of ab173298.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AKAP-associated Sperm Protein also known as ROPN1L functions as a regulatory protein in spermatozoa. It has a molecular mass of approximately 24 kDa and exhibits expression in testicular tissue particularly in the sperm flagella. As a member of the A-kinase anchoring proteins (AKAPs) family it binds to protein kinase A (PKA) thereby localizing it to specific subcellular compartments. This localization is vital for the regulation of intracellular signaling pathways and is fundamental to the functions that the sperm must perform.

Biological function summary

AKAP-associated Sperm Protein plays an important role in sperm motility and structural integrity. It participates as part of a protein complex involving other proteins that modulate the actin cytoskeleton important for cellular movement and stability. Its interactions within this complex significantly affect the dynamics of cellular scaffolding where it helps maintain the proper organization and facilitates signal transduction linked to motility.

Pathways

AKAP-associated Sperm Protein integrates into signaling pathways that regulate cAMP levels. This protein links with the cyclic adenosine monophosphate (cAMP) signaling pathway which is important for the regulation of energy metabolism and neurotransmitter actions. Other proteins it interacts with include protein kinase A (PKA) and other signaling intermediates which together guide signal transduction impacting cellular responses and function particularly in reproductive processes.

AKAP-associated Sperm Protein connects to infertility issues and ciliary dyskinesia. Abnormal function of this target protein can lead to compromised sperm motility which is often linked to male fertility problems. In the case of ciliary dyskinesia the protein may interact with other related proteins like DYX1C1 leading to defects in ciliary movement. These dysfunctions underline the significance of proper AKAP-associated Sperm Protein activity within reproductive health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia. Important for male fertility. With ROPN1, involved in fibrous sheath integrity and sperm motility, plays a role in PKA-dependent signaling processes required for spermatozoa capacitation.

See full target information ROPN1L

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

Immunoprecipitation - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

Western blot - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] - BSA and Azide free (AB249759)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise