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AB156004

Anti-AKAP1 antibody [EPR10518]

3

(2 Reviews)

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(2 Publications)

Rabbit Recombinant Monoclonal AKAP1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

AKAP149, PRKA1, AKAP1, A-kinase anchor protein 149 kDa, Dual specificity A-kinase-anchoring protein 1, Protein kinase A-anchoring protein 1, Spermatid A-kinase anchor protein 84, AKAP 149, D-AKAP-1, S-AKAP84

3 Images
Flow Cytometry (Intracellular) - Anti-AKAP1 antibody [EPR10518] (AB156004)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-AKAP1 antibody [EPR10518] (AB156004)

Intracellular flow cytometrical analysis of permeabilized 293T cells with ab156004 antibody at a dilution of 1/100 (red) compared to a negative rabbit IgG (green).

Western blot - Anti-AKAP1 antibody [EPR10518] (AB156004)
  • WB

Lab

Western blot - Anti-AKAP1 antibody [EPR10518] (AB156004)

False colour image of Western blot : Anti-AKAP1 antibody [EPR10518] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab156004 was shown to bind specifically to AKAP1. A band was observed at 125 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in AKAP1 knockout cell line ab266182 (knockout cell lysate ab257828). To generate this image, wild-type and AKAP1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-AKAP1 antibody [EPR10518] (ab156004) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

AKAP1 knockout HEK-293T cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

NIH/3T3 cell lysate at 20 µg

Predicted band size: 97 kDa

Observed band size: 125 kDa

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Western blot - Anti-AKAP1 antibody [EPR10518] (AB156004)
  • WB

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Western blot - Anti-AKAP1 antibody [EPR10518] (AB156004)

All lanes:

Western blot - Anti-AKAP1 antibody [EPR10518] (ab156004) at 1/1000 dilution

Lane 1:

SW480 cell lysate at 10 µg

Lane 2:

Caco-2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 97 kDa

Observed band size: 149 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10518

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

A-kinase anchoring protein 1 (AKAP1) also known as AKAP121 or D-AKAP1 is a protein that serves as an anchor point for signaling molecules. AKAP1 is approximately 121 kDa in mass. It is an important player in coordinating cellular processes by anchoring protein kinase A (PKA) to specific cellular compartments such as mitochondria. The protein is mainly expressed in the heart liver and brain but also found in other tissues indicating its broad role in cellular function.
Biological function summary

AKAP1 connects with several components to influence cellular signaling pathways and energy metabolism. It is a part of a complex network that involves mitochondrial targeting sequences which guide proteins to correct mitochondrial compartments. AKAP1 helps regulate processes like mitochondrial dynamics apoptosis and oxidative phosphorylation. These functions ensure efficient cellular energy production and maintenance of cellular health.

Pathways

AKAP1 engages in both the cAMP-dependent and mitochondrial signaling pathways. In the cAMP-dependent pathway AKAP1 interacts with PKA to modulate phosphorylation events that alter cellular responses to external signals. The mitochondrial pathway involves communication with proteins like Drp1 influencing mitochondrial fission and fusion processes. These interactions highlight AKAP1's central role in maintaining cellular energy balance and integrity.

Certain pathways involving AKAP1 link closely with neurodegenerative diseases such as Alzheimer's and cardiac disorders. During neurodegeneration AKAP1-related mitochondrial dysfunction can lead to energy deficits and increased oxidative stress contributing to disease progression. Additionally in cardiac health dysregulation of AKAP1 interactions with PKA can affect heart muscle contractility and lead to cardiac anomalies. Understanding these connections offers insights into potential therapeutic avenues for targeting such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane (By similarity). Involved in mitochondrial-mediated antiviral innate immunity (PubMed : 31522117). Promotes translocation of NDUFS1 into mitochondria to regulate mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) activity (By similarity).
See full target information AKAP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 20:388 PubMed33193848

2020

Hypoxia-induced circular RNA hsa_circ_0008450 accelerates hepatocellular cancer progression via the miR-431/AKAP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiajun Du,Jie Han,Shan Gao,Shangdi Zhang,Yunyan Pan

International journal of hypertension 2016:9146870 PubMed28097020

2016

Renal Urotensin II System Plays Roles in the Regulation of Blood Pressure in Dahl Salt-Resistant Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Wu,Guanjong Chen,Aihua Zhang,Yang Yu,Minhua Fan,Chaoshu Tang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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