JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB198895

Anti-AKAP12 antibody - C-terminal

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal AKAP12 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human AKAP12.

View Alternative Names

AKAP250, AKAP12, A-kinase anchor protein 12, AKAP-12, A-kinase anchor protein 250 kDa, Gravin, Myasthenia gravis autoantigen, AKAP 250

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB198895)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB198895)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling AKAP12 with ab198895 at 1/40 dilution.

Western blot - Anti-AKAP12 antibody - C-terminal (AB198895)
  • WB

Supplier Data

Western blot - Anti-AKAP12 antibody - C-terminal (AB198895)

8% SDS-PAGE

All lanes:

Western blot - Anti-AKAP12 antibody - C-terminal (ab198895) at 1/600 dilution

All lanes:

HT29 cell lysate at 40 µg

Predicted band size: 191 kDa

false

Exposure time: 40s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human AKAP12. The exact immunogen used to generate this antibody is proprietary information.

Q02952

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKAP12 also known as A-kinase anchoring protein 12 or Gravin is a scaffolding protein that orchestrates the spatial and temporal coordination of signaling pathways. It has a molecular mass of approximately 150 kilodaltons and is primarily expressed in various tissues including the heart brain and skeletal muscle. AKAP12 functions by anchoring protein kinase A (PKA) and other signaling molecules to specific cellular compartments enabling efficient signal transduction. The protein’s structural adaptability allows for the assembly of multiprotein complexes which are important for its mechanical role in cells.
Biological function summary

The functions of AKAP12 extend beyond simple scaffolding. It interacts with other components to form macromolecular complexes that regulate cellular processes such as cytoskeletal dynamics cell cycle progression and signal transduction. By binding to PKA and other kinases such as protein kinase C (PKC) AKAP12 modulates phosphorylation events essential for maintaining cellular homeostasis. It also plays roles in regulating gene expression and cellular responses to external stimuli influencing processes like cell migration and proliferation.

Pathways

AKAP12 significantly contributes to the regulation of the MAPK/ERK and PI3K/Akt signaling pathways. Within these pathways AKAP12 acts by organizing multiple signaling proteins into functional networks influencing cell growth and survival. It closely interacts with components like Raf and PKB/Akt facilitating effective signal relay. These interactions ensure tight regulation of key cellular activities including growth differentiation and survival which are pivotal for normal cellular function.

Research implicates AKAP12 in cancer progression and cardiovascular diseases. It appears to act as a tumor suppressor in certain cancers where its downregulation or loss correlates with poor prognosis and increased metastatic potential. Its interaction with proteins like integrins further suggests a role in promoting or inhibiting cancer cell migration and invasion. Additionally AKAP12's involvement in cardiovascular diseases relates to its function in vascular smooth muscle cell migration and the maintenance of endothelial barrier function potentially interacting with proteins such as endothelial nitric oxide synthase (eNOS).
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
See full target information AKAP12
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 20:20220912 PubMed40226362

2025

AKAP12, mediated by transcription factor 21, inhibits cell proliferation, metastasis, and glycolysis in lung squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Chen,Hehe Liao,Kaibin Wang,Tan Yan,Shaofei Ma,Guodong Bai

European journal of medical research 27:205 PubMed36253873

2022

Construction of mRNA prognosis signature associated with differentially expressed genes in early stage of stomach adenocarcinomas based on TCGA and GEO datasets.

Applications

Unspecified application

Species

Unspecified reactive species

Fuquan Jiang,Haiguan Lin,Hongfeng Yan,Xiaomin Sun,Jianwu Yang,Manku Dong

Journal of the American Society of Nephrology : JASN 33:1033-1044 PubMed35321939

2022

Hematopoietic Stem Cell Transplant-Membranous Nephropathy Is Associated with Protocadherin FAT1.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjeev Sethi,Benjamin Madden,Marta Casal Moura,Samih H Nasr,Nattawat Klomjit,LouAnn Gross,Vivian Negron,M Cristine Charlesworth,Mariam P Alexander,Nelson Leung,Ulrich Specks,Fernando C Fervenza,Mark Haas

Archives of medical science : AMS 17:462-473 PubMed33747281

2019

MiR-338-3p improved lung adenocarcinoma by suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Chang,Shuo Liu,Baowei Li,Zhongchao Huo,Xiaomin Wang,Hui Zhang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB204559

Anti-AKAP12 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB204559)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB202123

Anti-Gastrin Releasing Peptide antibody

Western blot - Anti-Gastrin Releasing Peptide antibody (AB202123)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB73288

Anti-GDF6 antibody

Western blot - Anti-GDF6 antibody (AB73288)

  • 5
  • 4
Primary Antibodies

AB245578

Anti-MAP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB245578)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com