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AB204559

Anti-AKAP12 antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal AKAP12 antibody. C-terminal. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AKAP12 aa 1600 to C-terminus.

View Alternative Names

AKAP250, AKAP12, A-kinase anchor protein 12, AKAP-12, A-kinase anchor protein 250 kDa, Gravin, Myasthenia gravis autoantigen, AKAP 250

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB204559)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB204559)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling AKAP12 with ab204559 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-AKAP12 antibody - C-terminal (AB204559)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AKAP12 antibody - C-terminal (AB204559)

Immunofluorescence analysis of U-251 MG cells labeling AKAP12 with ab204559 at 4 μg/ml.

Fixation/Permeabilization : PFA/Triton X-100

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB204559)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB204559)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling AKAP12 with ab204559 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB204559)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB204559)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling AKAP12 with ab204559 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB204559)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP12 antibody - C-terminal (AB204559)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling AKAP12 with ab204559 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human AKAP12 aa 1600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02952

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKAP12 also known as A-kinase anchoring protein 12 or Gravin is a scaffolding protein that orchestrates the spatial and temporal coordination of signaling pathways. It has a molecular mass of approximately 150 kilodaltons and is primarily expressed in various tissues including the heart brain and skeletal muscle. AKAP12 functions by anchoring protein kinase A (PKA) and other signaling molecules to specific cellular compartments enabling efficient signal transduction. The protein’s structural adaptability allows for the assembly of multiprotein complexes which are important for its mechanical role in cells.
Biological function summary

The functions of AKAP12 extend beyond simple scaffolding. It interacts with other components to form macromolecular complexes that regulate cellular processes such as cytoskeletal dynamics cell cycle progression and signal transduction. By binding to PKA and other kinases such as protein kinase C (PKC) AKAP12 modulates phosphorylation events essential for maintaining cellular homeostasis. It also plays roles in regulating gene expression and cellular responses to external stimuli influencing processes like cell migration and proliferation.

Pathways

AKAP12 significantly contributes to the regulation of the MAPK/ERK and PI3K/Akt signaling pathways. Within these pathways AKAP12 acts by organizing multiple signaling proteins into functional networks influencing cell growth and survival. It closely interacts with components like Raf and PKB/Akt facilitating effective signal relay. These interactions ensure tight regulation of key cellular activities including growth differentiation and survival which are pivotal for normal cellular function.

Research implicates AKAP12 in cancer progression and cardiovascular diseases. It appears to act as a tumor suppressor in certain cancers where its downregulation or loss correlates with poor prognosis and increased metastatic potential. Its interaction with proteins like integrins further suggests a role in promoting or inhibiting cancer cell migration and invasion. Additionally AKAP12's involvement in cardiovascular diseases relates to its function in vascular smooth muscle cell migration and the maintenance of endothelial barrier function potentially interacting with proteins such as endothelial nitric oxide synthase (eNOS).
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
See full target information AKAP12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 13:1064-1075 PubMed28453381

2017

Degradation of protein translation machinery by amino acid starvation-induced macroautophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Christine Gretzmeier,Sven Eiselein,Gregory R Johnson,Rudolf Engelke,Heike Nowag,Mostafa Zarei,Victoria Küttner,Andrea C Becker,Kristoffer T G Rigbolt,Maria Høyer-Hansen,Jens S Andersen,Christian Münz,Robert F Murphy,Jörn Dengjel
View all publications
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