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AB250165

Anti-AKAP14 antibody [EPR12955] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal AKAP14 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AKAP28, AKAP14, A-kinase anchor protein 14, AKAP-14, A-kinase anchor protein 28 kDa, Protein kinase A-anchoring protein 14, AKAP 28, PRKA14

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (AB250165)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (AB250165)

This data was developed using ab180160, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of A549 cells labeling AKAP14 with ab180160 at a 1/100 dilution.

Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (AB250165)
  • IP

Supplier Data

Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (AB250165)

This data was developed using ab180160, the same antibody clone in a different buffer formulation.

Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing AKAP14, using ab180160 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - C-terminal (<a href='/en-us/products/primary-antibodies/akap14-antibody-epr12955-c-terminal-ab180160'>ab180160</a>)

Predicted band size: 23 kDa

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Western blot - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (AB250165)
  • WB

Supplier Data

Western blot - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (AB250165)

This data was developed using ab180160, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AKAP14 antibody [EPR12955] - C-terminal (<a href='/en-us/products/primary-antibodies/akap14-antibody-epr12955-c-terminal-ab180160'>ab180160</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

NCI-H460 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Lane 5:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12955

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250165 is the carrier-free version of ab180160.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKAP14 also known as A-kinase anchor protein 14 plays an essential role in anchoring protein kinase A (PKA) to specific intracellular compartments. It weighs approximately 100 kDa. Researchers found it primarily in the centrosome and mitochondria. The protein enables the localization and functions of PKA which modulates various cellular processes. Through its capacity to tether PKA AKAP14 facilitates targeted phosphorylation of substrates impacting cellular signaling dynamics and structural integrity.
Biological function summary

AKAP14 helps organize and maintain the structural framework of cells by anchoring PKA in cellular compartments. As part of a larger protein complex AKAP14 interacts with other structural proteins facilitating their assembly and function. This function is important for maintaining centrosome integrity and proper mitochondrial function. The anchoring partners of AKAP14 ensure precise PKA activity where required affecting cell cycle regulation and energy metabolism.

Pathways

AKAP14 functions in PKA-dependent phosphorylation pathways that influence intracellular signaling. In coordination with other proteins it plays roles in the cAMP signaling pathway which regulates cell growth differentiation and apoptosis. AKAP14's interaction with proteins like RII the regulatory subunit of PKA enables the tight modulation of signaling pathways essential for these cellular processes. Another significant pathway involving AKAP14 is the mitotic spindle formation pathway where it ensures centrosome stability and chromosome segregation.

AKAP14's dysfunction is linked to specific mitochondrial-related disorders and certain types of cancer. Disruption in its function can result in cellular signaling imbalance contributing to mitochondrial myopathy due to impaired energy metabolism. Furthermore AKAP14's involvement in centrosome regulation associates it with cancers such as breast cancer where centrosome abnormalities are common. Mutations that affect its interaction with other centrosomal proteins like ASPM could lead to anomalies in cell division promoting oncogenic processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
See full target information AKAP14
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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