Anti-AKAP4 antibody [EPR21123]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP4 antibody [EPR21123] (AB230954)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling AKAP4 with ab230954 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining in spermatids of human testis (PMID : 19139142) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP4 antibody [EPR21123] (AB230954)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling AKAP4 with ab230954 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) Secondary antibody.

Negative control : Negative staining in human lung (PMID : 19139142).

Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-AKAP4 antibody [EPR21123] (AB230954)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human testis tissue. Panel A : Merged staining of anti-AKAP4 (magenta; Opal™690), anti-TPTE (green; Opal™520) and anti-IGF2BP1/IMP1 (red; Opal™570) on human testis. Panel B : Anti-IGF2BP1/IMP1 stained on spermatogonia. Panel C : Anti-TPTE stained on spermatocytes. Panel D : Anti-AKAP4 stained on spermatids. The section was incubated in three rounds of staining : in the order of ab238887, ab250148, and ab290736 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.

• WB

Supplier Data

Western blot - Anti-AKAP4 antibody [EPR21123] (AB230954)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AKAP4 antibody [EPR21123] (ab230954) at 1/1000 dilution

All lanes:

Human testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 94 kDa

false

Exposure time: 6s