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AB32679

Anti-AKAP9 antibody [17G10]

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(2 Publications)

Mouse Monoclonal AKAP9 antibody. Suitable for ICC, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AKAP9 aa 1-50.

View Alternative Names

AKAP350, AKAP450, KIAA0803, AKAP9, A-kinase anchor protein 9, AKAP-9, A-kinase anchor protein 350 kDa, A-kinase anchor protein 450 kDa, AKAP 120-like protein, Centrosome- and Golgi-localized PKN-associated protein, Protein hyperion, Protein kinase A-anchoring protein 9, Protein yotiao, AKAP 350, hgAKAP 350, AKAP 450, CG-NAP, PRKA9

4 Images
Immunocytochemistry - Anti-AKAP9 antibody [17G10] (AB32679)
  • ICC

Supplier Data

Immunocytochemistry - Anti-AKAP9 antibody [17G10] (AB32679)

Immunocytochemical analysis of AKAP9 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1 : 20 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunocytochemistry - Anti-AKAP9 antibody [17G10] (AB32679)
  • ICC

Supplier Data

Immunocytochemistry - Anti-AKAP9 antibody [17G10] (AB32679)

Immunocytochemical analysis of AKAP9 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1 : 100 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunocytochemistry - Anti-AKAP9 antibody [17G10] (AB32679)
  • ICC

Supplier Data

Immunocytochemistry - Anti-AKAP9 antibody [17G10] (AB32679)

Immunocytochemical analysis of AKAP9 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1 : 100 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP9 antibody [17G10] (AB32679)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP9 antibody [17G10] (AB32679)

Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1 : 20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

17G10

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC

applications

Immunogen

Synthetic Peptide within Human AKAP9 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q99996

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "4 µg/mL", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "4 µg/mL", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as A-kinase anchor protein 9 (AKAP9) also called Yotiao is involved in the regulation of protein kinase A (PKA). AKAP9 with a mass of approximately 454 kDa is expressed in multiple tissues including the brain heart and muscle tissue. This protein serves as a scaffolding protein that binds PKA to specific subcellular locations thereby organizing the action of signaling enzymes to their target sites.
Biological function summary

AKAP9 plays an essential role in coordinating cellular signaling events. It acts as a hub in a protein complex that includes PKA protein phosphatase 1 and other signaling molecules facilitating targeted signal transduction. It helps regulate ion channel function and the organization of the cell's cytoskeleton. In neurons AKAP9 modulates synaptic signaling and plasticity which influences neurotransmitter release.

Pathways

AKAP9 interacts with signaling pathways such as the cAMP-dependent pathway and calcium signaling. Through these pathways AKAP9 modulates the activity of several related proteins including PKA itself and other proteins like RyR2 a calcium channel. Its involvement in these pathways indicates important roles in regulating cardiac rhythm and other neuronal functions by targeting enzymes to their specific substrates.

AKAP9 has links to cardiac arrhythmias and neurodegenerative disorders. Long QT syndrome linked to aberrant calcium handling involves AKAP9 through its relationship with RyR2 and other cardiac proteins. In Alzheimer's disease the protein's role in synaptic signaling might connect it to amyloid beta and Tau proteins suggesting its involvement in neuronal dysfunction and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus (PubMed : 10202149, PubMed : 15047863). Required for microtubule nucleation at the cis-side of the Golgi apparatus (PubMed : 15047863, PubMed : 19242490). Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed : 25657325). In complex with PDE4DIP isoform 13/MMG8/SMYLE, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed : 27666745, PubMed : 28814570). In complex with PDE4DIP isoform 13/MMG8/SMYLE, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension also from the centrosome to the cell periphery (PubMed : 29162697).. Isoform 4. Associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.
See full target information AKAP9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 13:933963 PubMed35837016

2022

Loss of Unconventional Myosin VI Affects cAMP/PKA Signaling in Hindlimb Skeletal Muscle in an Age-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lilya Lehka,Dominika Wojton,Małgorzata Topolewska,Vira Chumak,Łukasz Majewski,Maria Jolanta Rędowicz

Cancer management and research 14:157-167 PubMed35046723

2022

AKAP9 Upregulation Predicts Unfavorable Prognosis in Pediatric Acute Myeloid Leukemia and Promotes Stemness Properties via the Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shiwen Wu,Dongqin Shen,Li Zhao
View all publications
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