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Rabbit Recombinant Monoclonal AKR1B10 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 4 publications.

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Images

Western blot - Anti-AKR1B10 antibody [EPR14421] (AB192865), expandable thumbnail
  • Western blot - Anti-AKR1B10 antibody [EPR14421] (AB192865), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (AB192865), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (AB192865), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody [EPR14421] (AB192865), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/500 - 1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed:9565553, PubMed:18087047, PubMed:12732097, PubMed:19013440, PubMed:19563777). Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:18087047). Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:19013440, PubMed:19563777). Displays no reductase activity towards glucose (PubMed:12732097).

Alternative names

Aldo-keto reductase family 1 member B10, ARL-1, Aldose reductase-like, Aldose reductase-related protein, Small intestine reductase, ARP, hARP, SI reductase, AKR1B11, AKR1B10

Recommended products

Rabbit Recombinant Monoclonal AKR1B10 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR14421

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aldo-keto reductase family 1 member B10 (AKR1B10) also known as ARL-1 (aldo-keto reductase-like-1) is an enzyme encoded by the AKR1B10 gene. The enzyme has a molecular weight of approximately 36 kDa. It is mainly expressed in the liver gastrointestinal tract and to a lesser extent in the lung and adrenal gland. As an enzyme AKR1B10 catalyzes the reduction of carbonyl groups to their corresponding alcohols using NADPH as a cofactor. This function plays a role in the detoxification of aldehydes and ketones.

Biological function summary

AKR1B10 protects cells from disorders caused by toxic aldehydes. It is important in lipid biosynthesis through the reduction of retinaldehyde to retinol which is part of the retinoic acid metabolism. AKR1B10 forms part of the aldo-keto reductase superfamily consisting of multiple related enzymes that regulate various cellular processes. This enzyme class is involved in stabilizing the oxidoreduction balance within cells.

Pathways

AKR1B10 engages in lipid metabolism and the protection against oxidative stress. It is an important player in the retinoid metabolism and signaling pathway where it collaborates with enzymes like retinaldehyde dehydrogenase. Additionally AKR1B10 is involved in the polyol pathway although its exact role here continues to be studied. The enzyme's relationship with NADPH suggests it could intersect with systems managing cellular responses to oxidative stress.

Associated diseases and disorders

AKR1B10 is associated with certain cancers and complications related to chronic alcohol consumption. Overexpression of AKR1B10 has been linked to hepatocellular carcinoma where it possibly collaborates with proteins like the carcinogenic beta-catenin pathway. Furthermore it plays a part in promoting resistance to chemotherapeutic drugs due to its detoxification role. In liver diseases caused by alcohol AKR1B10 helps protect hepatocytes from oxidative and aldehyde stress with aldehyde dehydrogenase being another involved enzyme in these processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Western blot - Anti-AKR1B10 antibody [EPR14421] (ab192865), expandable thumbnail

    Western blot - Anti-AKR1B10 antibody [EPR14421] (ab192865)

    Blocking buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-AKR1B10 antibody [EPR14421] (ab192865) at 1/10000 dilution

    All lanes: A549 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

    Observed band size: 36 kDa

  • Western blot - Anti-AKR1B10 antibody [EPR14421] (ab192865), expandable thumbnail

    Western blot - Anti-AKR1B10 antibody [EPR14421] (ab192865)

    Blocking buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-AKR1B10 antibody [EPR14421] (ab192865) at 1/1000 dilution

    All lanes: SW480 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

    Observed band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (ab192865), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (ab192865)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling AKR1B10 with ab192865 at a 1/1000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (ab192865), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1B10 antibody [EPR14421] (ab192865)

    Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling AKR1B10 using ab192865 at a 1/1,000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody [EPR14421] (ab192865), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AKR1B10 antibody [EPR14421] (ab192865)

    Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cells labeling AKR1B10 with ab192865 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

  • Flow Cytometry (Intracellular) - Anti-AKR1B10 antibody [EPR14421] (ab192865), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-AKR1B10 antibody [EPR14421] (ab192865)

    Intracellular flow cytometric analysis of A549 cells labeling AKR1B10 using ab192865 at a 1/50 dilution (red). Goat anti rabbit IgG (FITC) was used as the secondary antibody at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control cells without incubation with primary antibody and secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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