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Mouse Monoclonal AKR1C2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

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Images

Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (AB131375), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (AB131375), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 0.877% Sodium chloride, 0.357% HEPES

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
Cow
Predicted
Predicted
Rabbit
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
2 µg/mL
Notes

-

Species
Rat
Dilution info
2 µg/mL
Notes

-

Species
Human
Dilution info
2 µg/mL
Notes

-

Predicted
Predicted

Species
Rabbit, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

(paraformaldehyde fixed cells)

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rabbit, Cow
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed:19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed:14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed:10998348). Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol) (PubMed:15929998, PubMed:17034817, PubMed:17442338, PubMed:8573067). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT (PubMed:10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed:19218247). Displays affinity for bile acids (PubMed:8486699).

Alternative names

DDH2, AKR1C2, Aldo-keto reductase family 1 member C2, 3-alpha-HSD3, Chlordecone reductase homolog HAKRD, Dihydrodiol dehydrogenase 2, Dihydrodiol dehydrogenase/bile acid-binding protein, Type III 3-alpha-hydroxysteroid dehydrogenase, DD-2, DD2, DD/BABP

Recommended products

Mouse Monoclonal AKR1C2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
4B6AF3
Concentration
Loading...
Purification notes

Purity >90% by SDS-PAGE.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The AKR1C1 and AKR1C2 are members of the aldo-keto reductase family. These enzymes also known as dihydrodiol dehydrogenases reduce a variety of substrates including steroids prostaglandins and xenobiotics. AKR1C1 has a molecular mass of approximately 37 kDa while AKR1C2 has a similar but slightly distinct mass. These proteins are expressed in various tissues including the liver prostate and mammary glands where they perform their enzymatic functions.

Biological function summary

The AKR1C1/AKR1C2 enzymes play significant roles in steroid hormone metabolism. They modulate the conversion of active hormones to their inactive forms and vice versa affecting the cellular concentration of these hormones. The proteins do not function as part of a larger complex but influence multiple biochemical processes through their enzymatic actions. Their roles in the regulation of sex steroids are important for maintaining normal cellular activity and tissue homeostasis.

Pathways

AKR1C1 and AKR1C2 are involved in intricate steroid hormone signaling and metabolism pathways. They engage with the androgen and estrogen receptor signaling pathways by modulating hormone availability. The interplay with enzymes like 5-alpha reductase and aromatase highlights their role in the balance of active and inactive hormone forms within these pathways. Their function can influence cellular proliferation and differentiation processes within different tissues.

Associated diseases and disorders

AKR1C1/AKR1C2 have links to hormone-related cancers such as prostate and breast cancer. These enzymes impact the local hormone levels affecting cancer progression and response to therapy. Alterations in AKR1C1 and AKR1C2 expression or function can lead to hormone imbalances influencing cancer risk and treatment outcomes. Through their modulation of steroid hormones they connect to the activity of proteins like androgen and estrogen receptors which are critical in the pathogenesis of hormone-driven cancers.

Product promise

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2 product images

  • Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375), expandable thumbnail

    Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375)

    0.3% PBST was used as a blocking buffer in all incubation steps. The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the Goat anti-Mouse secondary antibody.

    All lanes: Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375) at 2 µg/mL

    Lane 1: Molecular weight marker

    Lane 2: HeLa Cell Lysate at 15 µg

    Lane 3: HepG2 Cell Lysate at 15 µg

    Lane 4: Human Liver Homogenate (HLH) at 10 µg

    Lane 5: Human Heart Homogenate (HHH) at 10 µg

    Lane 6: Mouse Liver Homogenate (MLH) at 10 µg

    Lane 7: Rat Liver Homogenate (RLH) at 10 µg

    Lane 8: Rat Heart Homogenate (RHH) at 10 µg

    Secondary

    All lanes: Goat anti-Mouse AP at 1/3000 dilution

    Predicted band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375)

    Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 μg/ml.

    The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.

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Product protocols

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