AB131375

Anti-AKR1C1/AKR1C2 antibody [4B6AF3]

Name: Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375) | Abcam
Brand: Abcam Limited
SKU: AB131375
Price: 505 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal AKR1C2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

DDH2, AKR1C2, Aldo-keto reductase family 1 member C2, 3-alpha-HSD3, Chlordecone reductase homolog HAKRD, Dihydrodiol dehydrogenase 2, Dihydrodiol dehydrogenase/bile acid-binding protein, Type III 3-alpha-hydroxysteroid dehydrogenase, DD-2, DD2, DD/BABP

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (AB131375)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (AB131375)

Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 μg/ml.

The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor^® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.

Unknown

Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (AB131375)

0.3% PBST was used as a blocking buffer in all incubation steps. The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the Goat anti-Mouse secondary antibody.

All lanes:

Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375) at 2 µg/mL

Lane 1:

Molecular weight marker

Lane 2:

HeLa Cell Lysate at 15 µg

Lane 3:

HepG2 Cell Lysate at 15 µg

Lane 4:

Human Liver Homogenate (HLH) at 10 µg

Lane 5:

Human Heart Homogenate (HHH) at 10 µg

Lane 6:

Mouse Liver Homogenate (MLH) at 10 µg

Lane 7:

Rat Liver Homogenate (RLH) at 10 µg

Lane 8:

Rat Heart Homogenate (RHH) at 10 µg

Secondary

All lanes:

Goat anti-Mouse AP at 1/3000 dilution

Predicted band size: 37 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4B6AF3

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "(paraformaldehyde fixed cells)" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification notes

Purity >90% by SDS-PAGE.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 0.877% Sodium chloride, 0.357% HEPES

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AKR1C1 and AKR1C2 are members of the aldo-keto reductase family. These enzymes also known as dihydrodiol dehydrogenases reduce a variety of substrates including steroids prostaglandins and xenobiotics. AKR1C1 has a molecular mass of approximately 37 kDa while AKR1C2 has a similar but slightly distinct mass. These proteins are expressed in various tissues including the liver prostate and mammary glands where they perform their enzymatic functions.

Biological function summary

The AKR1C1/AKR1C2 enzymes play significant roles in steroid hormone metabolism. They modulate the conversion of active hormones to their inactive forms and vice versa affecting the cellular concentration of these hormones. The proteins do not function as part of a larger complex but influence multiple biochemical processes through their enzymatic actions. Their roles in the regulation of sex steroids are important for maintaining normal cellular activity and tissue homeostasis.

Pathways

AKR1C1 and AKR1C2 are involved in intricate steroid hormone signaling and metabolism pathways. They engage with the androgen and estrogen receptor signaling pathways by modulating hormone availability. The interplay with enzymes like 5-alpha reductase and aromatase highlights their role in the balance of active and inactive hormone forms within these pathways. Their function can influence cellular proliferation and differentiation processes within different tissues.

AKR1C1/AKR1C2 have links to hormone-related cancers such as prostate and breast cancer. These enzymes impact the local hormone levels affecting cancer progression and response to therapy. Alterations in AKR1C1 and AKR1C2 expression or function can lead to hormone imbalances influencing cancer risk and treatment outcomes. Through their modulation of steroid hormones they connect to the activity of proteins like androgen and estrogen receptors which are critical in the pathogenesis of hormone-driven cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed : 19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed : 14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed : 10998348). Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol) (PubMed : 15929998, PubMed : 17034817, PubMed : 17442338, PubMed : 8573067). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT (PubMed : 10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed : 19218247). Displays affinity for bile acids (PubMed : 8486699).

See full target information AKR1C2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular pain 18:17448069211069255 PubMed35040378

2022

Sigma-1 receptors and progesterone metabolizing enzymes in nociceptive sensory neurons of the female rat trigeminal ganglia: A neural substrate for the antinociceptive actions of progesterone.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca S Hornung,Namrata Gr Raut,Daisy J Cantu,Lauren M Lockhart,Dayna L Averitt

Oncology research 22:203-211 PubMed26351209

2015

AEG-1 Promotes Metastasis Through Downstream AKR1C2 and NF1 in Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Li,Xia Wu,Wei Zhang,Jia Li,Huawei Liu,Ming Hao,Junsong Wang,Honghai Zhang,Gengxia Yang,Meijun Hao,Shoupeng Sheng,Yu Sun,Jiang Long,Xiongbing Hu,Hanshuo Zhang,Caixia Hu,Li Li,Jiasheng Zheng

Journal of biomolecular screening 21:101-7 PubMed26318406

2015

High-Content Functional Screening of AEG-1 and AKR1C2 for the Promotion of Metastasis in Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Li,Xia Wu,Wei Zhang,Jia Li,Huawei Liu,Ming Hao,Junsong Wang,Honghai Zhang,Gengxia Yang,Meijun Hao,Shoupeng Sheng,Yu Sun,Jiang Long,Juan Li,Fengfeng Zhuang,Caixia Hu,Li Li,Jiasheng Zheng

Drug metabolism and disposition: the biological fate of chemicals 43:1019-27 PubMed25904761

2015

Metabolism of bupropion by carbonyl reductases in liver and intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie N Connarn,Xinyuan Zhang,Andrew Babiskin,Duxin Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-AKR1C1/AKR1C2 antibody [4B6AF3]

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (AB131375)

Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (AB131375)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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