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AB251405

Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal AKR1C3 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DDH1, HSD17B5, KIAA0119, PGFS, AKR1C3, Aldo-keto reductase family 1 member C3, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-hydroxysteroid dehydrogenase type 2, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase 3, Dihydrodiol dehydrogenase type I, HA1753, Prostaglandin F synthase, Testosterone 17-beta-dehydrogenase 5, 17-beta-HSD 5, 3-alpha-HSD type 2, DD-3, DD3

5 Images
Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)
  • WB

Lab

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)

Blocking and diluting buffer : 5% NFDM/TBST This data was developed using ab203834, the same antibody clone in a different buffer formulation

All lanes:

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (<a href='/en-us/products/primary-antibodies/akr1c1akr1c2akr1c3-antibody-epr16725-ab203834'>ab203834</a>) at 1/1000 dilution

Lane 1:

His-tagged human AKR1C1 recombinant protein, full-length

Lane 2:

His-tagged human AKR1C2 recombinant protein, full-length

Lane 3:

His-tagged human AKR1C3 recombinant protein, full-length

Lane 4:

His-tagged human AKR1C4 recombinant protein, full-length

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Observed band size: 39 kDa

false

Exposure time: 40s

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)
  • WB

Unknown

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)

This data was developed using ab203834, the same antibody clone in a different buffer formulation.

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 5% NFDM /TBST or 1% BSA/TBST.

All lanes:

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (<a href='/en-us/products/primary-antibodies/akr1c1akr1c2akr1c3-antibody-epr16725-ab203834'>ab203834</a>) at 1/10000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Exposure time: 3min

Immunoprecipitation - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)
  • IP

Supplier Data

Immunoprecipitation - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)

This data was developed using ab203834, the same antibody clone in a different buffer formulation.

AKR1C3 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab203834 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203834 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : Human fetal liver whole cell lysate 10ug (Input). Lane 2 : ab203834 IP in Human fetal liver whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203834 in Human fetal liver whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (<a href='/en-us/products/primary-antibodies/akr1c1akr1c2akr1c3-antibody-epr16725-ab203834'>ab203834</a>)

Predicted band size: 37 kDa

false

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)
  • WB

Supplier Data

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)

This data was developed using ab203834, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (<a href='/en-us/products/primary-antibodies/akr1c1akr1c2akr1c3-antibody-epr16725-ab203834'>ab203834</a>) at 1/10000 dilution

All lanes:

MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Exposure time: 3min

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)
  • WB

Supplier Data

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] - BSA and Azide free (AB251405)

This data was developed using ab203834, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (<a href='/en-us/products/primary-antibodies/akr1c1akr1c2akr1c3-antibody-epr16725-ab203834'>ab203834</a>) at 1/5000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16725

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251405 is the carrier-free version of ab203834.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AKR1C3 AKR1C2 and AKR1C1 proteins collectively known as aldo-keto reductase family 1 members C3 C2 and C1 are enzymes that convert carbonyl groups into alcohols in various biological processes. AKR1C3 also called 17β-hydroxysteroid dehydrogenase type 5 has a mass of approximately 37 kDa. These proteins are found in several tissues including liver prostate and mammary glands. They play a role in steroid hormone metabolism and are essential in regulating androgen progesterone and prostaglandin levels.
Biological function summary

The AKR1C3 enzyme modifies steroid hormones impacting cell proliferation and differentiation. AKR1C2 and AKR1C1 have similar functions but can affect other substances such as bile acids and xenobiotics. Together they do not form a single complex but act in overlapping and multiple pathways collectively influencing hormone levels and metabolic processes. Their activity affects the balance of active and inactive forms of various hormones contributing to normal cellular function.

Pathways

AKR1C3 AKR1C2 and AKR1C1 participate in steroid hormone biosynthesis and retinoic acid metabolism. They interrelate with enzymes like cytochrome P450 in the synthesis and breakdown of hormones and retinoids. This association affects cellular signaling and gene expression particularly in response to hormone fluctuations. Their integration into these pathways highlights their contribution to homeostasis and the intricate regulation of biochemical processes.

These proteins have connections to prostate cancer and breast cancer. Their role in androgen and estrogen metabolism influences tumor growth and progression. In prostate cancer AKR1C3 interacts with androgen receptor signaling intensifying the disease state. For breast cancer it affects estrogen receptor pathways where altered expression may lead to hormone-resistant forms of cancer. Understanding these associations aids in identifying therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone (PubMed : 10622721, PubMed : 11165022, PubMed : 7650035, PubMed : 9415401, PubMed : 9927279). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed : 11165022, PubMed : 14672942). Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone (PubMed : 20036328). Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol (PubMed : 10622721, PubMed : 10998348, PubMed : 11165022, PubMed : 15047184, PubMed : 19010934, PubMed : 20036328). Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed : 10557352, PubMed : 10998348, PubMed : 11165022, PubMed : 14672942, PubMed : 7650035, PubMed : 9415401). Also displays retinaldehyde reductase activity toward 9-cis-retinal (PubMed : 21851338).
See full target information AKR1C3

Additional targets

AKR1C2,AKR1C1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3596 PubMed34155205

2021

ERRγ enhances cardiac maturation with T-tubule formation in human iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Miki,Kohei Deguchi,Misato Nakanishi-Koakutsu,Antonio Lucena-Cacace,Shigeru Kondo,Yuya Fujiwara,Takeshi Hatani,Masako Sasaki,Yuki Naka,Chikako Okubo,Megumi Narita,Ikue Takei,Stephanie C Napier,Tsukasa Sugo,Sachiko Imaichi,Taku Monjo,Tatsuya Ando,Norihisa Tamura,Kenichi Imahashi,Tomoyuki Nishimoto,Yoshinori Yoshida
View all publications
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Product promise

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