AB320772

Anti-AKR1C3 + AKR1C1 antibody [EPR28747-30] - BSA and Azide free (Detector)

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal AKR1C3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

DDH1, HSD17B5, KIAA0119, PGFS, AKR1C3, Aldo-keto reductase family 1 member C3, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-hydroxysteroid dehydrogenase type 2, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase 3, Dihydrodiol dehydrogenase type I, HA1753, Prostaglandin F synthase, Testosterone 17-beta-dehydrogenase 5, 17-beta-HSD 5, 3-alpha-HSD type 2, DD-3, DD3, DDH, DDH1, AKR1C1, Aldo-keto reductase family 1 member C1, 20alpha-hydroxysteroid dehydrogenase, 3beta-hydroxysteroid 3-dehydrogenase, Chlordecone reductase homolog HAKRC, Dihydrodiol dehydrogenase 1, High-affinity hepatic bile acid-binding protein, 20-alpha-HSD, DD1, HBAB

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Sandwich ELISA - Anti-AKR1C3 + AKR1C1 antibody [EPR28747-30] - BSA and Azide free (Detector) (AB320772)

sELISA

Supplier Data

Sandwich ELISA - Anti-AKR1C3 + AKR1C1 antibody [EPR28747-30] - BSA and Azide free (Detector) (AB320772)

Sandwich ELISA of ab320770 with the capture antibody ab320771 dilution at 2 ug/mL and detector antibody ab320772 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28747-30

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has 100% cross reaction with human AKR1C1 and 61% cross reaction with human AKR1C4.

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Reactivity data

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Product details

ab320772 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human AKR1C3. The recommended pair for sandwich ELISA is:

Capture: ab320771, Human AKR1C3 Capture Antibody (unconjugated)

Detector: ab320772, Human AKR1C3 Detector Antibody (unconjugated)

The reference range value is 39.06-5000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKR1C3 and AKR1C1 are members of the aldo-keto reductase family. These enzymes also known as human type 5 17β-hydroxysteroid dehydrogenases (17β-HSD5) play roles in steroid metabolism. AKR1C3 has a mass of about 37 kDa while AKR1C1 weighs around 36 kDa. They catalyze the reduction of ketones and aldehydes to their corresponding alcohols leveraging NADPH as a cofactor. Both proteins are expressed in a variety of tissues such as the liver prostate and mammary glands.

Biological function summary

AKR1C3 and AKR1C1 are involved in hormone regulation by converting active steroids into their inactive forms and vice versa. They do not typically operate as part of large complexes but still have significant roles. AKR1C3 for example converts androstenedione to testosterone therefore impacting androgen signaling. AKR1C1 on the other hand transforms progesterone into an inactive metabolite thereby regulating progestin levels.

Pathways

AKR1C3 and AKR1C1 are integral to the androgen and progestin biosynthesis pathways. They play critical roles in the detoxification of aldehydes and hormones in these pathways. AKR1C3 is associated with the conversion of prostaglandin D2 to its inactive form PGF2α altering inflammatory responses. These enzymes are linked functionally to cytochrome P450 enzymes such as CYP19A1 which are involved in hormone synthesis and metabolism.

AKR1C3 and AKR1C1 show significant implications in hormone-dependent cancers and metabolic disorders. AKR1C3 is highly expressed in prostate cancer where it enables increased levels of active androgens promoting cancer cell proliferation. This protein is also involved in polycystic ovary syndrome (PCOS) owing to its role in steroid metabolism. In breast cancer AKR1C1 contributes to the disease's progression by altering progesterone levels working alongside estrogen receptors in modulating cell growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytosolic aldo-keto reductase that catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids. Displays broad substrate specificity with distinct positional and stereochemistry, primarily generating 17beta-hydroxysteroids, but also 3alpha- and 20alpha-hydroxysteroids (PubMed : 10998348, PubMed : 11165022, PubMed : 20036328, PubMed : 9415401, PubMed : 9927279, PubMed : 10998348, PubMed : 9927279). Produces potent androgens via classical and 'backdoor'/alternative pathways. In the classical androgen metabolic pathway (biosynthesis of 5alpha-dihydrotestosterone (5alpha-DHT) via testosterone), catalyzes the reduction of delta4-androstenedione to form testosterone (PubMed : 10998348, PubMed : 11165022, PubMed : 20036328, PubMed : 9415401, PubMed : 9927279). In the 'backdoor' androgen metabolic pathway (biosynthesis of 5alpha-dihydrotestosterone (5alpha-DHT) via pregnanes), reduces androsterone to 5alpha-androstane-3alpha,17beta-diol preceding 5alpha-DHT secretion (PubMed : 10557352, PubMed : 10998348, PubMed : 9415401). Reduces 5alpha-DHT to less potent androgen 5alpha-androstane-3alpha,17beta-diol, likely regulating ligand availability for androgen receptors (PubMed : 10557352, PubMed : 10998348, PubMed : 11165022, PubMed : 14672942, PubMed : 7650035, PubMed : 9415401). May contribute to the metabolism of adrenal-derived androgen precursors. Reduces 11-keto-4-androstene-3,17-dione (11KA4) and 11-keto-5alpha-androstane-3,17-dione (11K-Adione) into potent androgens 11-ketotestosterone (11KT) and 11-ketodihydrotestosterone (11KDHT), respectively (PubMed : 31926269). In estrogen metabolism, catalyzes the conversion of estrone to potent estrogen 17beta-estradiol (PubMed : 10998348, PubMed : 11165022, PubMed : 20036328). Acts as a prostaglandin (PG) F2alpha synthase. Displays 11-ketoreductase and 9,11-endoperoxide reductase activities and reduces PGD2 to 11beta-PGF2alpha and PGH2 to PGF2alpha (PubMed : 10622721, PubMed : 11165022, PubMed : 15047184, PubMed : 19010934, PubMed : 20036328, PubMed : 7650035, PubMed : 9415401, PubMed : 9927279). Also displays retinaldehyde reductase activity toward 9-cis-retinal (PubMed : 21851338). In vitro can efficiently catalyze bidirectional conversion between ketosteroids and hydroxysteroids using NADPH/NADP(+) or NADH/NAD(+) as cofactors. In vivo however, the reductase activity prevails since the major reducing cofactor NADPH inhibits NAD(+)-dependent oxidase activity (PubMed : 11165022, PubMed : 14672942). In addition, it is able to reduce in vitro various carbonyl compounds like menadione, phenanthrenequinone and nitrobenzaldehyde (By similarity).

See full target information AKR1C3

Additional targets

AKR1C1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AKR1C3 + AKR1C1 antibody [EPR28747-30] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-AKR1C3 + AKR1C1 antibody [EPR28747-30] - BSA and Azide free (Detector) (AB320772)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise