Rabbit Polyclonal AKR1C3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AKR1C3 aa 1-200.
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Publications
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- Steroids 143:73-792019Characterization of a highly specific monoclonal antibody against human aldo-keto reductase AKR1C3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayu Liu et. al.PubMed 30639543
- Scientific reports 6:199702016Intracrine Androgens Enhance Decidualization and Modulate Expression of Human Endometrial Receptivity Genes.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AKR1C3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P42330
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Associated Products
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Target data
Function
Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone (PubMed:10622721, PubMed:11165022, PubMed:7650035, PubMed:9415401, PubMed:9927279). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed:11165022, PubMed:14672942). Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone (PubMed:20036328). Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol (PubMed:10622721, PubMed:10998348, PubMed:11165022, PubMed:15047184, PubMed:19010934, PubMed:20036328). Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed:10557352, PubMed:10998348, PubMed:11165022, PubMed:14672942, PubMed:7650035, PubMed:9415401). Also displays retinaldehyde reductase activity toward 9-cis-retinal (PubMed:21851338).
Alternative names
DDH1, HSD17B5, KIAA0119, PGFS, AKR1C3, Aldo-keto reductase family 1 member C3, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-hydroxysteroid dehydrogenase type 2, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase 3, Dihydrodiol dehydrogenase type I, HA1753, Prostaglandin F synthase, Testosterone 17-beta-dehydrogenase 5, 17-beta-HSD 5, 3-alpha-HSD type 2, DD-3, DD3
Recommended products
Rabbit Polyclonal AKR1C3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AKR1C3 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AKR1C3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P42330
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The AKR1C3 protein also known as aldo-keto reductase family 1 member C3 functions as an enzyme and part of the aldo-keto reductase superfamily. AKR1C3 reduces ketosteroids and prostaglandins participating in steroid hormone metabolism. It has a molecular mass of about 37 kDa. It is expressed in various tissues such as the liver ovaries and adrenal glands.
- Biological function summary
The enzyme engages in important roles in androgen estrogen and prostaglandin metabolism. AKR1C3 works in steroid biosynthesis by reducing 17-ketosteroids to their hydroxysteroid forms. It participates in the regulation of sex steroid hormones and contributes to prostaglandin metabolism though it does not function as part of a complex. Its activity can affect the balance of hormones and local cellular processes impacting a variety of biological activities.
- Pathways
AKR1C3 plays significant roles in metabolic and signaling pathways including the steroid hormone biosynthesis and prostaglandin receptor pathways. It interacts with other enzymes like AKR1C2 which also works in steroid processing adding to the regulation of hormone levels and activities. These pathways enable the enzyme to alter cellular responses and influence receptor signaling.
- Associated diseases and disorders
AKR1C3 has associations with hormone-related cancers such as prostate and breast cancer. Its overexpression or misregulation could drive tumor progression by altering hormone levels that fuel cancer growth. It connects with other proteins like CYP19A1 (aromatase) involved in estrogen synthesis indicating a contribution to estrogen-sensitive cancer development. Overall AKR1C3's role in metabolic dysfunctions and hormone-related disorders emphasizes its importance as a biological target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (ab137546)
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, labelling AKR1C3 with ab137546 at 1/100 dilution.
Western blot - Anti-AKR1C3 antibody (ab137546)
10% SDS PAGE
All lanes: Western blot - Anti-AKR1C3 antibody (ab137546) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 30 µg
Lane 2: HepG2 whole cell lysate at 30 µg
Lane 3: Molt-4 whole cell lysate at 30 µg
Predicted band size: 37 kDa
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