JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB196673

Anti-AKR1C3 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal AKR1C3 antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AKR1C3.

View Alternative Names

DDH1, HSD17B5, KIAA0119, PGFS, AKR1C3, Aldo-keto reductase family 1 member C3, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-hydroxysteroid dehydrogenase type 2, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase 3, Dihydrodiol dehydrogenase type I, HA1753, Prostaglandin F synthase, Testosterone 17-beta-dehydrogenase 5, 17-beta-HSD 5, 3-alpha-HSD type 2, DD-3, DD3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (AB196673)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (AB196673)

Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling AKR1C3 with ab196673 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (AB196673)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (AB196673)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling AKR1C3 with ab196673 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (AB196673)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (AB196673)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling AKR1C3 with ab196673 at 1/100 dilution.

Western blot - Anti-AKR1C3 antibody (AB196673)
  • WB

Supplier Data

Western blot - Anti-AKR1C3 antibody (AB196673)

All lanes:

Western blot - Anti-AKR1C3 antibody (ab196673) at 1/500 dilution

Lane 1:

K562 cell extract

Lane 2:

Jurkat cell extract

Lane 3:

HepG2 cell extract

Lane 4:

A549 cell extract

Lane 5:

SW620 cell extract

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human AKR1C3.

P42330

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AKR1C3 protein also known as aldo-keto reductase family 1 member C3 functions as an enzyme and part of the aldo-keto reductase superfamily. AKR1C3 reduces ketosteroids and prostaglandins participating in steroid hormone metabolism. It has a molecular mass of about 37 kDa. It is expressed in various tissues such as the liver ovaries and adrenal glands.
Biological function summary

The enzyme engages in important roles in androgen estrogen and prostaglandin metabolism. AKR1C3 works in steroid biosynthesis by reducing 17-ketosteroids to their hydroxysteroid forms. It participates in the regulation of sex steroid hormones and contributes to prostaglandin metabolism though it does not function as part of a complex. Its activity can affect the balance of hormones and local cellular processes impacting a variety of biological activities.

Pathways

AKR1C3 plays significant roles in metabolic and signaling pathways including the steroid hormone biosynthesis and prostaglandin receptor pathways. It interacts with other enzymes like AKR1C2 which also works in steroid processing adding to the regulation of hormone levels and activities. These pathways enable the enzyme to alter cellular responses and influence receptor signaling.

AKR1C3 has associations with hormone-related cancers such as prostate and breast cancer. Its overexpression or misregulation could drive tumor progression by altering hormone levels that fuel cancer growth. It connects with other proteins like CYP19A1 (aromatase) involved in estrogen synthesis indicating a contribution to estrogen-sensitive cancer development. Overall AKR1C3's role in metabolic dysfunctions and hormone-related disorders emphasizes its importance as a biological target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone (PubMed : 10622721, PubMed : 11165022, PubMed : 7650035, PubMed : 9415401, PubMed : 9927279). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed : 11165022, PubMed : 14672942). Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone (PubMed : 20036328). Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol (PubMed : 10622721, PubMed : 10998348, PubMed : 11165022, PubMed : 15047184, PubMed : 19010934, PubMed : 20036328). Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed : 10557352, PubMed : 10998348, PubMed : 11165022, PubMed : 14672942, PubMed : 7650035, PubMed : 9415401). Also displays retinaldehyde reductase activity toward 9-cis-retinal (PubMed : 21851338).
See full target information AKR1C3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 181:3760-3778 PubMed38872396

2024

Factor XII and prekallikrein promote microvascular inflammation and psoriasis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yurong Zhang,Zengrong Chen,Junyan Guo,Qing Wan,Yingjie Zhang,Huihui Li,Haojie Rao,Jianfeng Yang,Pengfei Xu,Hong Chen,Miao Wang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB137546

Anti-AKR1C3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (AB137546)

  • 0
  • 0
Primary Antibodies

AB209899

Anti-AKR1C3+AKR1C4 antibody [EPR16726]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C3+AKR1C4 antibody [EPR16726] (AB209899)

  • 0
  • 0
Primary Antibodies

AB203834

Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725]

RabMAb|Recombinant

Western blot - Anti-AKR1C1+AKR1C2+AKR1C3 antibody [EPR16725] (AB203834)

  • 0
  • 0
Primary Antibodies

AB137545

Anti-AKR1C3 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C3 antibody - C-terminal (AB137545)

  • 0
  • 0
Primary Antibodies

AB84327

Anti-AKR1C3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C3 antibody (AB84327)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB131375

Anti-AKR1C1/AKR1C2 antibody [4B6AF3]

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (AB131375)

  • 0
  • 0
Biochemicals

AB141015

(S)-(+)-Ibuprofen, Non-selective COX inhibitor

Immunocytochemistry/ Immunofluorescence - (S)-(+)-Ibuprofen, Non-selective COX inhibitor (AB141015)

  • 0
  • 0
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com