Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)

Rabbit Recombinant Monoclonal AKR1C3 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Human, Mouse samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (AB316865), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	Flow Cyt (Intra)	IP	ICC/IF
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Expected	Expected	Expected
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Target data

See full target information AKR1C3

Function

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone (PubMed:10622721, PubMed:11165022, PubMed:7650035, PubMed:9415401, PubMed:9927279). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed:11165022, PubMed:14672942). Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone (PubMed:20036328). Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol (PubMed:10622721, PubMed:10998348, PubMed:11165022, PubMed:15047184, PubMed:19010934, PubMed:20036328). Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed:10557352, PubMed:10998348, PubMed:11165022, PubMed:14672942, PubMed:7650035, PubMed:9415401). Also displays retinaldehyde reductase activity toward 9-cis-retinal (PubMed:21851338).

Alternative names

DDH1, HSD17B5, KIAA0119, PGFS, AKR1C3, Aldo-keto reductase family 1 member C3, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-hydroxysteroid dehydrogenase type 2, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase 3, Dihydrodiol dehydrogenase type I, HA1753, Prostaglandin F synthase, Testosterone 17-beta-dehydrogenase 5, 17-beta-HSD 5, 3-alpha-HSD type 2, DD-3, DD3

Recommended products

Rabbit Recombinant Monoclonal AKR1C3 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28747-41
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab316865 is the carrirer-free version of Anti-AKR1C3 antibody [EPR28747-41] ab316864.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The AKR1C3 protein also known as aldo-keto reductase family 1 member C3 functions as an enzyme and part of the aldo-keto reductase superfamily. AKR1C3 reduces ketosteroids and prostaglandins participating in steroid hormone metabolism. It has a molecular mass of about 37 kDa. It is expressed in various tissues such as the liver ovaries and adrenal glands.

Biological function summary

The enzyme engages in important roles in androgen estrogen and prostaglandin metabolism. AKR1C3 works in steroid biosynthesis by reducing 17-ketosteroids to their hydroxysteroid forms. It participates in the regulation of sex steroid hormones and contributes to prostaglandin metabolism though it does not function as part of a complex. Its activity can affect the balance of hormones and local cellular processes impacting a variety of biological activities.

Pathways

AKR1C3 plays significant roles in metabolic and signaling pathways including the steroid hormone biosynthesis and prostaglandin receptor pathways. It interacts with other enzymes like AKR1C2 which also works in steroid processing adding to the regulation of hormone levels and activities. These pathways enable the enzyme to alter cellular responses and influence receptor signaling.

Associated diseases and disorders

AKR1C3 has associations with hormone-related cancers such as prostate and breast cancer. Its overexpression or misregulation could drive tumor progression by altering hormone levels that fuel cancer growth. It connects with other proteins like CYP19A1 (aromatase) involved in estrogen synthesis indicating a contribution to estrogen-sensitive cancer development. Overall AKR1C3's role in metabolic dysfunctions and hormone-related disorders emphasizes its importance as a biological target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Western blot - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
AKR1C3 Western blot staining using rabbit Anti-AKR1C3 antibody
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Low expression:HCT 116 (PMID: 21215737, PMID: 35388063)
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-AKR1C3 antibody [EPR28747-41] (Anti-AKR1C3 antibody [EPR28747-41] ab316864) at 1/1000 dilution
Lane 1: TF-1 (human Erythroleukemia erythroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 4: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 36 kDa, 124 kDa
Exposure time: 10s
Dot Blot - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
AKR1C3 Dot Blot staining using rabbit Anti-AKR1C3 antibody
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Dot blot analysis of AKR1C3 using Anti-AKR1C3 antibody [EPR28747-41] ab316864 (Lane 1-4) at 1/1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 Dilution] dilution and Dot blot analysis of AKR1C3 using Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 (lane 5-8) at 1/1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution.
Lane 1&5: His tagged AKR1C1 recombinant protein
Lane 2&6: His tagged AKR1C2 recombinant protein
Lane 3&7: AKR1C3 recombinant protein without tag
Lane 4&8: His tagged AKR1C4 recombinant protein
Lanes 1 - 4: Dot Blot - Anti-AKR1C3 antibody [EPR28747-41] (Anti-AKR1C3 antibody [EPR28747-41] ab316864) at 1/1000 dilution
Lanes 5 - 8: Dot Blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) at 1/1000 dilution
Lanes 1 and 5: His tagged AKR1C1 recombinant protein
Lanes 2 and 6: His tagged AKR1C2 recombinant protein
Lanes 3 and 7: AKR1C3 recombinant protein without tag
Lanes 4 and 8: His tagged AKR1C4 recombinant protein
Secondary
All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Exposure time: 180s
Western blot - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Low expression: muscle (PMID: 18635746)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-AKR1C3 antibody [EPR28747-41] (Anti-AKR1C3 antibody [EPR28747-41] ab316864) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse kidney tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Mouse skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 36 kDa
Exposure time: 10s
Western blot - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Low expression: muscle (PMID: 18635746)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-AKR1C3 antibody [EPR28747-41] (Anti-AKR1C3 antibody [EPR28747-41] ab316864) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human kidney tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 36 kDa
Exposure time: 8s
Immunoprecipitation - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
AKR1C3 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate

Lane 2: Anti-AKR1C3 antibody [EPR28747-41] ab316864 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-AKR1C3 antibody [EPR28747-41] ab316864 in A549 whole cell lysate
All lanes: Immunoprecipitation - Anti-AKR1C3 antibody [EPR28747-41] (Anti-AKR1C3 antibody [EPR28747-41] ab316864) at 1/30 dilution
All lanes: A549 (human lung carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 6s
Flow Cytometry (Intracellular) - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell, Left) / TF-1 (human erythroleukemia erythroblast, Right) cells labelling AKR1C3 with Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: HCT 116.
Immunocytochemistry/ Immunofluorescence - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized TF-1 (human erythroleukemia erythroblast) cells labelling AKR1C3 with Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/50 (10.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing nuclear and cytoplasmic staining in TF-1 cell line.

Low expression: HCT 116 (PMID: 21215737).

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling AKR1C3 with Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on mouse cardiac muscle.

The section was incubated with Anti-AKR1C3 antibody [EPR28747-41] ab316864 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling AKR1C3 with Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on human cardiac muscle (PMID: 33352741).

The section was incubated with Anti-AKR1C3 antibody [EPR28747-41] ab316864 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling AKR1C3 with Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse colon.

The section was incubated with Anti-AKR1C3 antibody [EPR28747-41] ab316864 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling AKR1C3 with Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse liver.

The section was incubated with Anti-AKR1C3 antibody [EPR28747-41] ab316864 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AKR1C3 with Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human hepatocellular carcinoma.

The section was incubated with Anti-AKR1C3 antibody [EPR28747-41] ab316864 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody [EPR28747-41] - BSA and Azide free (ab316865)
This data was developed using Anti-AKR1C3 antibody [EPR28747-41] ab316864, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling AKR1C3 with Anti-AKR1C3 antibody [EPR28747-41] ab316864 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon (PMID: 33352741).

The section was incubated with Anti-AKR1C3 antibody [EPR28747-41] ab316864 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins