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AB251511

Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal AKR1C3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DDH1, HSD17B5, KIAA0119, PGFS, AKR1C3, Aldo-keto reductase family 1 member C3, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-hydroxysteroid dehydrogenase type 2, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase 3, Dihydrodiol dehydrogenase type I, HA1753, Prostaglandin F synthase, Testosterone 17-beta-dehydrogenase 5, 17-beta-HSD 5, 3-alpha-HSD type 2, DD-3, DD3

7 Images
Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)
  • WB

Lab

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)

Blocking and diluting buffer : 5% NFDM/TBST This data was developed using ab209899, the same antibody clone in a different buffer formulation.

Lanes 1 - 4:

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] (<a href='/en-us/products/primary-antibodies/akr1c3akr1c4-antibody-epr16726-ab209899'>ab209899</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (ab251511) at 1/1000 dilution

Lane 1:

His-tagged human AKR1C1 recombinant protein, full-length

Lane 2:

His-tagged human AKR1C2 recombinant protein, full-length

Lane 3:

His-tagged human AKR1C3 recombinant protein, full-length

Lane 4:

His-tagged human AKR1C4 recombinant protein, full-length

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Observed band size: 39 kDa

false

Exposure time: 10s

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)

This data was developed using ab209899, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling AKR1C3 with ab209899 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on HepG2 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab209899 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)

This data was developed using ab209899, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A549 (Human lung carcinoma cell line) cells labeling AKR1C3 with ab209899 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)

This data was developed using ab209899, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling AKR1C3 with ab209899 at 1/100 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on A549 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (Alexa Fluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab209899 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunoprecipitation - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)
  • IP

Supplier Data

Immunoprecipitation - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)

This data was developed using ab209899, the same antibody clone in a different buffer formulation.

AKR1C3 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab209899 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab209899 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HepG2 whole cell lysate, 10μg (Input).
Lane 2 : ab209899 IP in HepG2 whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab209899 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-AKR1C3+AKR1C4 antibody [EPR16726] (<a href='/en-us/products/primary-antibodies/akr1c3akr1c4-antibody-epr16726-ab209899'>ab209899</a>)

Predicted band size: 37 kDa

false

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)
  • WB

Supplier Data

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)

This data was developed using ab209899, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1 and 3 : 2 seconds; Lane 2 : 30 seconds.

Lanes 1 - 2:

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] (<a href='/en-us/products/primary-antibodies/akr1c3akr1c4-antibody-epr16726-ab209899'>ab209899</a>) at 1/1000 dilution

Lane 3:

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] (<a href='/en-us/products/primary-antibodies/akr1c3akr1c4-antibody-epr16726-ab209899'>ab209899</a>) at 1/5000 dilution

Lane 1:

Human fetal kidney lysate at 20 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 37 kDa,90 kDa

Observed band size: 37 kDa

false

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)
  • WB

Supplier Data

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] - BSA and Azide free (AB251511)

This data was developed using ab209899, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AKR1C3+AKR1C4 antibody [EPR16726] (<a href='/en-us/products/primary-antibodies/akr1c3akr1c4-antibody-epr16726-ab209899'>ab209899</a>) at 1/2000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (control) at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with AKR1C3 peptide on the membrane

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with AKR1C4 peptide on the membrane

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16726

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab251511 is the carrier-free version of ab209899.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proteins AKR1C3 and AKR1C4 also known as Aldo-Keto Reductase Family 1 Member C3 and C4 are reductases involved in the metabolism of steroids prostaglandins and xenobiotics. AKR1C3 has a molecular mass of approximately 37 kDa while AKR1C4 is slightly larger at around 37.2 kDa. These proteins are expressed mainly in the liver but you can also find AKR1C3 in adrenal glands and prostate tissues. Current research shows their functions in enzymatic reduction processes significantly affect hormone conversion and lipid metabolism.
Biological function summary

The proteins AKR1C3 and AKR1C4 have significant roles in steroid hormone metabolism. They do not form a part of any larger complex but act independently to catalyze the conversion of active androgens and estrogens. AKR1C3 for example converts androstenedione to testosterone and estrone to estradiol affecting steroid balance in tissues. Meanwhile AKR1C4 primarily catalyzes the reduction of bile acid intermediates assisting in maintaining liver health and function.

Pathways

AKR1C3 and AKR1C4 operate closely in metabolic pathways involving steroid metabolism and bile acid biosynthesis. In the steroid metabolism pathway they associate with other enzymes like 5α-reductase and CYP19A1 (aromatase) to regulate the conversion and amplification of steroid hormones. In bile acid biosynthesis AKR1C4 works conjointly with enzymes like CYP7A1 playing a significant role in processing cholesterol substrates into bile acids important for lipid digestion and absorption.

AKR1C3 and AKR1C4 have associations with conditions such as prostate cancer and liver disease. AKR1C3's upregulation correlates with prostate cancer progression by influencing androgen receptor activation. It relates to enzymes like CYP17A1 which share involvement in androgen biosynthesis. Meanwhile AKR1C4 plays a role in liver diseases such as cholestasis and is thought to interact with proteins involved in bile transport and synthesis potentially impacting pathological bile acid accumulation. Understanding these interactions helps inform therapeutic strategies targeting hormone and bile acid-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone (PubMed : 10622721, PubMed : 11165022, PubMed : 7650035, PubMed : 9415401, PubMed : 9927279). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed : 11165022, PubMed : 14672942). Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone (PubMed : 20036328). Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol (PubMed : 10622721, PubMed : 10998348, PubMed : 11165022, PubMed : 15047184, PubMed : 19010934, PubMed : 20036328). Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed : 10557352, PubMed : 10998348, PubMed : 11165022, PubMed : 14672942, PubMed : 7650035, PubMed : 9415401). Also displays retinaldehyde reductase activity toward 9-cis-retinal (PubMed : 21851338).
See full target information AKR1C3

Additional targets

AKR1C4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 18:545-552 PubMed31289526

2019

Lymphoepithelioma-like gastric carcinoma treated with partial gastrectomy: Two case reports.

Applications

Unspecified application

Species

Unspecified reactive species

Huihua Cao,Jun Xie,Yongxiang Qian,Yugang Wu,Zhaoqing Tang
View all publications
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