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AB197973

Anti-AKR1C4 antibody

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(1 Publication)

Rabbit Polyclonal AKR1C4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AKR1C4.

View Alternative Names

CHDR, AKR1C4, Aldo-keto reductase family 1 member C4, 3-alpha-hydroxysteroid dehydrogenase type I, 3alpha-hydroxysteroid 3-dehydrogenase, Chlordecone reductase, Dihydrodiol dehydrogenase 4, HAKRA, 3-alpha-HSD1, CDR, DD-4, DD4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C4 antibody (AB197973)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C4 antibody (AB197973)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue sections labeling AKR1C4 with ab197973 at a 1/30 dilution.

Western blot - Anti-AKR1C4 antibody (AB197973)
  • WB

Supplier Data

Western blot - Anti-AKR1C4 antibody (AB197973)

8% SDS-PAGE

All lanes:

Western blot - Anti-AKR1C4 antibody (ab197973) at 1/400 dilution

All lanes:

mouse liver tissue lysate at 40 µg

Predicted band size: 37 kDa

false

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human AKR1C4.

P17516

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKR1C4 also known as Aldo-keto reductase family 1 member C4 plays a role in the metabolism of steroid hormones. It acts mainly as a ketosteroid reductase involved in the conversion of aldo-keto forms. The protein has a molecular mass of approximately 37 kDa. AKR1C4 is expressed in several tissues with high levels found in the liver. In the liver it contributes to the oxidative inactivation of various steroid hormones thereby maintaining hormonal balance.
Biological function summary

AKR1C4 functions to reduce ketosteroids into their respective alcohol forms. This activity is important in steroid metabolism and detoxification processes. AKR1C4 is not part of any major enzyme complexes but its function is essential for processing steroids within cells. It impacts hormonal activity by converting potent steroids into their less active forms facilitating the regulation of physiological responses.

Pathways

AKR1C4 is part of the steroid hormone metabolic pathway and the bile acid biosynthesis pathway. In steroid hormone metabolism it interacts closely with proteins such as AKR1C3 which also acts on similar substrates. Its activity in bile acid biosynthesis ensures the proper conversion and excretion of bile acids essential for lipid digestion and absorption.

AKR1C4 involvement has been noted in liver diseases and hormone-related disorders. Dysregulation or altered expression of AKR1C4 can contribute to conditions such as hepatic steatosis and endocrine disorders like polycystic ovary syndrome (PCOS). Its interaction with proteins like the androgen receptor is also significant as it modulates androgen availability and activity potentially impacting androgen-sensitive conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Liver specific enzyme that acts as an NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain (PubMed : 10634139, PubMed : 10998348, PubMed : 11158055, PubMed : 14672942, PubMed : 1530633, PubMed : 19218247, PubMed : 7650035). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed : 14672942). Acts preferentially as a 3-alpha-hydroxysteroid dehydrogenase (HSD) with a subsidiary 3-beta-HSD activity (PubMed : 14672942). Catalyzes efficiently the transformation of the potent androgen 5-alpha-dihydrotestosterone (5alpha-DHT or 17beta-hydroxy-5alpha-androstan-3-one) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed : 10998348, PubMed : 11158055, PubMed : 14672942). Catalyzes the reduction of estrone into 17beta-estradiol but with low efficiency (PubMed : 14672942). Metabolizes a broad spectrum of natural and synthetic therapeutic steroid and plays an important role in metabolism of androgens, estrogens, progestereone and conjugated steroids (PubMed : 10998348, PubMed : 14672942, PubMed : 19218247). Catalyzes the biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leading to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route (PubMed : 2427522).
See full target information AKR1C4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:2135-2151.e7 PubMed38848692

2024

eIF4F complex dynamics are important for the activation of the integrated stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Kyusik Q Kim,Ankanahalli N Nanjaraj Urs,Victor Lasehinde,Alison C Greenlaw,Benjamin H Hudson,Hani S Zaher
View all publications
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