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AB97458

Anti-AKR7A2 antibody

5

(1 Review)

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(1 Publication)

Rabbit Polyclonal AKR7A2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AKR7A2 aa 50 to C-terminus.

View Alternative Names

AFAR, AFAR1, AKR7, AKR7A2, Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, Aldoketoreductase 7, Succinic semialdehyde reductase, AFB1-AR 1, SSA reductase

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-AKR7A2 antibody (AB97458)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AKR7A2 antibody (AB97458)

ab97458, at a 1/200 dilution, staining AKR7A2 in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR7A2 antibody (AB97458)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR7A2 antibody (AB97458)

Immunohistochemical analysis of PFA fixed, paraffin-embedded mouse fore brain tissue labeling AKR7A2 with a97458 at 1/500 dilution.

Western blot - Anti-AKR7A2 antibody (AB97458)
  • WB

Unknown

Western blot - Anti-AKR7A2 antibody (AB97458)

10% SDS PAGE

All lanes:

Western blot - Anti-AKR7A2 antibody (ab97458) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AKR7A2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43488

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKR7A2 also known as aflatoxin aldehyde reductase or succinic semialdehyde reductase is an enzyme with a molecular mass of approximately 38 kDa. It is part of the aldo-keto reductase superfamily and catalyzes the reduction of aldehydes and ketones to their corresponding alcohols using NAD(P)H as a cofactor. AKR7A2 is expressed in various tissues with significant levels in the liver kidney and lungs indicating its involvement in detoxification processes.
Biological function summary

AKR7A2 participates in the detoxification of harmful aldehydes and builds up resistance against oxidative stress. This enzymatic activity helps in converting reactive aldehydes which are formed during lipid peroxidation into less harmful compounds. AKR7A2 does not form part of a larger enzymatic complex but interacts with other molecules within the cell to fulfill its protective roles. By metabolizing these aldehydes AKR7A2 contributes to safeguarding cellular integrity and function.

Pathways

AKR7A2 is involved in cellular defense mechanisms and detoxification pathways. It plays a role in the glutathione metabolism pathway important for handling oxidative damage in cells. AKR7A2 also intersects with pathobiological pathways related to aldehyde detoxification. Its activity correlates with proteins such as glutathione S-transferases (GSTs) which together help neutralize and excrete toxic substances from the body maintaining cellular health.

AKR7A2 has links to cancer and neurodegenerative diseases. High levels of this enzyme are associated with increased capacity to detoxify carcinogenic aldehydes which could contribute to resistance against chemotherapy in certain cancers. Additionally alterations in AKR7A2 activity are seen in neurodegenerative disorders due to its pivotal role in protecting neurons from oxidative stress. Proteins like aldehyde dehydrogenases (ALDHs) also participate in these oxidative pathways and have connections with AKR7A2 in such disease contexts highlighting the enzyme's importance in disease prevention and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
See full target information AKR7A2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 128:323-340 PubMed29202474

2017

ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Prachi Mishra,Wei Tang,Vasanta Putluri,Tiffany H Dorsey,Feng Jin,Fang Wang,Donewei Zhu,Lauren Amable,Tao Deng,Shaofei Zhang,J Keith Killian,Yonghong Wang,Tsion Z Minas,Harry G Yfantis,Dong H Lee,Arun Sreekumar,Michael Bustin,Wei Liu,Nagireddy Putluri,Stefan Ambs
View all publications
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