Rabbit Polyclonal AKR7A2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AKR7A2 aa 50 to C-terminus.
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Publications
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- The Journal of clinical investigation 128:323-3402017ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrachi Mishra,Wei Tang,Vasanta Putluri,Tiffany H Dorsey,Feng Jin,Fang Wang,Donewei Zhu,Lauren Amable,Tao Deng,Shaofei Zhang,J Keith Killian,Yonghong Wang,Tsion Z Minas,Harry G Yfantis,Dong H Lee,Arun Sreekumar,Michael Bustin,Wei Liu,Nagireddy Putluri,Stefan AmbsPubMed 29202474
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AKR7A2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43488
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Target data
Function
Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
Alternative names
AFAR, AFAR1, AKR7, AKR7A2, Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, Aldoketoreductase 7, Succinic semialdehyde reductase, AFB1-AR 1, SSA reductase
Recommended products
Rabbit Polyclonal AKR7A2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AKR7A2 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AKR7A2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43488
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AKR7A2 also known as aflatoxin aldehyde reductase or succinic semialdehyde reductase is an enzyme with a molecular mass of approximately 38 kDa. It is part of the aldo-keto reductase superfamily and catalyzes the reduction of aldehydes and ketones to their corresponding alcohols using NAD(P)H as a cofactor. AKR7A2 is expressed in various tissues with significant levels in the liver kidney and lungs indicating its involvement in detoxification processes.
- Biological function summary
AKR7A2 participates in the detoxification of harmful aldehydes and builds up resistance against oxidative stress. This enzymatic activity helps in converting reactive aldehydes which are formed during lipid peroxidation into less harmful compounds. AKR7A2 does not form part of a larger enzymatic complex but interacts with other molecules within the cell to fulfill its protective roles. By metabolizing these aldehydes AKR7A2 contributes to safeguarding cellular integrity and function.
- Pathways
AKR7A2 is involved in cellular defense mechanisms and detoxification pathways. It plays a role in the glutathione metabolism pathway important for handling oxidative damage in cells. AKR7A2 also intersects with pathobiological pathways related to aldehyde detoxification. Its activity correlates with proteins such as glutathione S-transferases (GSTs) which together help neutralize and excrete toxic substances from the body maintaining cellular health.
- Associated diseases and disorders
AKR7A2 has links to cancer and neurodegenerative diseases. High levels of this enzyme are associated with increased capacity to detoxify carcinogenic aldehydes which could contribute to resistance against chemotherapy in certain cancers. Additionally alterations in AKR7A2 activity are seen in neurodegenerative disorders due to its pivotal role in protecting neurons from oxidative stress. Proteins like aldehyde dehydrogenases (ALDHs) also participate in these oxidative pathways and have connections with AKR7A2 in such disease contexts highlighting the enzyme's importance in disease prevention and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-AKR7A2 antibody (ab97458)
10% SDS PAGE
All lanes: Western blot - Anti-AKR7A2 antibody (ab97458) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR7A2 antibody (ab97458)
ab97458, at a 1/200 dilution, staining AKR7A2 in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR7A2 antibody (ab97458)
Immunohistochemical analysis of PFA fixed, paraffin-embedded mouse fore brain tissue labeling AKR7A2 with a97458 at 1/500 dilution.
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