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AB227231

Anti-AKR7A3 antibody

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(3 Publications)

Rabbit Polyclonal AKR7A3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AKR7A3.

View Alternative Names

AFAR2, AKR7A3, Aldo-keto reductase family 7 member A3, AFB1 aldehyde reductase 2, Aflatoxin B1 aldehyde reductase member 3, AFB1-AR 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR7A3 antibody (AB227231)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR7A3 antibody (AB227231)

Paraffin-embedded CL1-5 xenograft tissue stained for AKR7A3 using ab227231 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-AKR7A3 antibody (AB227231)
  • WB

Supplier Data

Western blot - Anti-AKR7A3 antibody (AB227231)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-AKR7A3 antibody (ab227231) at 1/1000 dilution

All lanes:

HEK-293T (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human AKR7A3. The exact immunogen used to generate this antibody is proprietary information.

O95154

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKR7A3 also known as aflatoxin aldehyde reductase is an enzyme that functions mechanically to reduce aldehyde substrates to alcohols. This protein weighs approximately 36 kDa and is expressed widely in human tissues with higher levels in the liver and kidneys. AKR7A3 belongs to the aldo-keto reductase superfamily which is involved in detoxifying reactive carbonyl species playing an important role in protecting cells from oxidative stress.
Biological function summary

The detoxifying enzyme AKR7A3 significantly contributes to cellular defense mechanisms by converting toxic aldehydes such as those from lipid peroxidation into less harmful alcohols. It does not commonly form part of any protein complex but interacts indirectly with other detoxification enzymes to maintain cellular redox balance. Its activity supports cell survival under conditions of oxidative stress by mitigating the damaging effects of reactive aldehyde intermediates.

Pathways

AKR7A3 is instrumental in the metabolism of xenobiotics and the inflammatory response pathways. It plays a role in pathways alongside enzymes like glutathione S-transferase contributing to the detoxification of electrophiles and reactive aldehydes. This protective action reduces cellular oxidative stress functioning in tandem with enzymes involved in glutathione metabolism to safeguard cellular components from damage.

AKR7A3 has associations with liver pathologies including hepatocellular carcinoma and metabolic disorders involving oxidative stress. It shares a connection with proteins like Nrf2 which regulates the expression of antioxidant proteins and contributes to the cellular response against oxidative damage. Altered expression or function of AKR7A3 can impact the detoxification processes potentially leading to increased susceptibility to liver disease and cancer development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NADPH-dependent reduction of various carbonyl-containing compounds, including aldehydes, ketones, and toxic products from cellular metabolism or environmental exposure. Can reduce the dialdehyde form of aflatoxin B1 (AFB1) into alcohol derivatives, via monoaldehydes intermediates. Can reduce the dialdehyde form of aflatoxin B1 (AFB1) into alcohol derivatives, via monoaldehydes intermediates, thus preventing the formation of protein adducts that contribute to AFB1-induced toxicity.
See full target information AKR7A3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed41007649

2025

Inhibiting Suppresses Cell Proliferation by Upregulating the Apoptotic Pathway in Multiple Myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Tao,Lihua Yuan,Yuntian Ding,Rongli Xie,Fangjie Liu,Zhongming Zhang,Xiaojun Xu,Xiaobo Wang

Frontiers in pharmacology 16:1534118 PubMed40365308

2025

Triptolide alleviates psoriasis through inhibiting the Wnt5a/β-Catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Eryang Chen,Lei Wang,Qu Wang,Yan Cai,Yaning Dou,Hongyan Qu,Junyi Zhu,Haiyang Zhao,Suqing Zheng,Chengguang Zhao,Bin Chen

Journal of hepatocellular carcinoma 8:997-1021 PubMed34513744

2021

Regulation Network and Prognostic Significance of Aldo-Keto Reductase (AKR) Superfamily Genes in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tianxing Dai,Linsen Ye,Haoyuan Yu,Kun Li,Jing Li,Rongqiang Liu,Xu Lu,Mingbin Deng,Rong Li,Wei Liu,Yang Yang,Guoying Wang
View all publications
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Product promise

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