Rabbit Polyclonal AKT3 phospho Y312 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 54 publications. Immunogen corresponding to Synthetic Peptide within Human AKT1 phospho Y315 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
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AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
AKT1 phospho Y315, RAC-beta serine/threonine-protein kinase phospho Y316
PKBG, AKT3, PKBG, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, RAC-PK-gamma, STK-2, PKB gamma
Rabbit Polyclonal AKT3 phospho Y312 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 54 publications. Immunogen corresponding to Synthetic Peptide within Human AKT1 phospho Y315 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
Liquid
Polyclonal
Affinity purification Immunogen
ab131443 detects endogenous levels of AKT1/2/3 only when phosphorylated at tyrosine 315/316/312.
ab131443 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Blue Ice
+4°C
-20°C
Stable for 12 months at -20°C
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This supplementary information is collated from multiple sources and compiled automatically.
AKT1 AKT2 and AKT3 also known as Protein Kinase B (PKB) isoforms are serine/threonine-specific protein kinases with critical roles in cellular processes. AKT1 has a molecular weight of about 55.8 kDa AKT2 weighs approximately 56.1 kDa and AKT3 typically has a similar mass. These proteins are expressed in many tissues including brain and heart with AKT1 ubiquitously present AKT2 focused in insulin-responsive tissues and AKT3 mainly in the brain. The molecular weight of AKT plays an important role in their functionality and specificity in tissues.
AKT proteins regulate cell cycle growing cell survival proliferation and metabolism. They participate as core components of the PI3K/AKT/mTOR signaling pathway forming complexes with other proteins to transmit signals. They bind to phosphoinositide lipids on the cell membrane facilitating their activation and downstream signaling. Through these activities the AKT transporter proteins maintain cellular homeostasis and play a part in stress response.
AKT proteins engage in important signaling networks including the PI3K/AKT pathway and mTOR pathway. They work closely with PI3K and mTOR proteins coordinating cellular growth and energy metabolism. In particular the AKT pathway responds to growth factors and insulin influencing glucose uptake and glycolysis regulation through interaction with proteins such as glycogen synthase kinase 3 (GSK3).
Dysregulation of AKT signaling can lead to cancer and diabetes. High AKT activation correlates with various cancers by promoting cell survival and growth. In diabetes impaired AKT2 regulation disrupts glucose uptake affecting insulin response. AKT's relationship with mTOR is significant as it often influences tumor growth and progression in cancerous tissues.
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All lanes: Western blot - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443) at 1/500 dilution
Lane 1: HepG2 cell extract untreated
Lane 2: HepG2 cell extract treated with EGF
Predicted band size: 56 kDa
Immunofluorescent analysis of methanol-fixed HeLa cells labelling AKT1 + AKT2 + AKT3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling AKT1 + AKT2 + AKT3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/50 dilution. Right panel was preincubated with blocking peptide.
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