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Rabbit Recombinant Monoclonal HDAC5 phospho S498 + S498 antibody. Carrier free. Suitable for IP, ELISA, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 1 publication.

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Images

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676), expandable thumbnail
  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676), expandable thumbnail
  • Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676), expandable thumbnail
  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676), expandable thumbnail
  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPELISADotWB
Human
Tested
Expected
Expected
Tested
Mouse
Expected
Expected
Expected
Tested
Rat
Expected
Expected
Expected
Tested
Synthetic peptide
Not recommended
Tested
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Target data

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed:28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed:28167758).

Alternative names

KIAA0600, HDAC5, KIAA0600, Histone deacetylase 5, HD5, Antigen NY-CO-9

Recommended products

Rabbit Recombinant Monoclonal HDAC5 phospho S498 + S498 antibody. Carrier free. Suitable for IP, ELISA, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR17680

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab250676 is the carrier-free version of Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

AKT1 also known as Protein Kinase B (PKB) is a serine/threonine kinase involved in various cellular processes. AKT1 plays an important role in mediating signals for cell survival growth and metabolism. This protein has a molecular weight of approximately 56 kDa. AKT1 is ubiquitously expressed in many tissues including the brain heart and lungs showing its importance in multiple physiological contexts. Phosphorylation of AKT1 at serine 473 denoted as p-AKT S473 is an important modification that regulates its activity.

Biological function summary

AKT1 regulates a broad spectrum of cellular functions through signaling pathways. It is an important player in the phosphatidylinositol 3-kinase (PI3K) and AKT pathway forming part of a complex involved in promoting cell survival and growth. AKT1 interacts closely with other proteins such as mTOR influencing cellular metabolism and autophagy. The phosphorylation state of AKT1 is critical for its activity with modifications like p-AKT S473 impacting its interaction with cellular substrates.

Pathways

AKT1 participates in the PI3K/AKT/mTOR signaling cascade instrumental in cell proliferation and metabolism. AKT1 integrates signals from insulin and growth factors modulating pathways that control cell growth and glucose uptake. This pathway involves proteins like mTOR and phospho-AKT which coordinate cellular responses to external stimuli. AKT1’s phosphorylation at serine 473 and the involvement of AKT1 E17K a gain-of-function mutation influence these pathways significantly.

Associated diseases and disorders

AKT1 has associations with cancer and metabolic disorders. Dysregulation of the PI3K/AKT/mTOR pathway involving AKT1 and mTOR often results in oncogenic transformation and uncontrolled cellular proliferation. In cancer such as breast cancer AKT1 mutations including AKT1 E17K are implicated altering cell signals for growth. Additionally AKT1 is connected to metabolic disorders such as Type 2 diabetes where it affects insulin signaling and glucose metabolism highlighting its interaction with metabolic proteins like mTOR and phospho-AKT.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676), expandable thumbnail

    Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676)

    This data was developed using Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556) at 1/1000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate, untreated at 10 µg

    Lane 2: MCF7 whole cell lysate, treated with Alkaline Phosphatase at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

    Observed band size: 56 kDa

    Exposure time: 15s

  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676), expandable thumbnail

    Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676)

    This data was developed using Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556) at 1/10000 dilution

    Lane 1: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate, untreated at 10 µg

    Lane 2: NIH/3T3 whole cell lysate, treated with Alkaline Phosphatase at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

    Observed band size: 56 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676), expandable thumbnail

    Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676)

    This data was developed using Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556, the same antibody clone in a different buffer formulation.

    Immunoprecipitation of AKT1 from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate achieved using Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556 at 1/100 dilution.

    Lane 1: Input: 10μg of MCF7 whole cell lysate.

    Lane 2: MCF7 whole cell lysate following IP with Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556.

    Lane 3: negative control: IP using Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556 in MCF7 whole cell lysate.

    Western blot was performed using Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556 at 1/1000 dilution.

    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] (Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556)

    Predicted band size: 47 kDa, 55 kDa

    Observed band size: 56 kDa

  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676), expandable thumbnail

    Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676)

    This data was developed using Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556) at 1/10000 dilution

    Lane 1: C6 (Rat glial tumor cells) whole cell lysate, untreated at 10 µg

    Lane 2: C6 whole cell lysate, treated with Alkaline Phosphatase at 10 µg

    Secondary

    All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

    Observed band size: 56 kDa

    Exposure time: 3min

  • Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676), expandable thumbnail

    Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676)

    This data was developed using Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556) at 1/1000 dilution

    Lane 1: Human fetal brain tissue lysate at 10 µg

    Lane 2: Human fetal kidney tissue lysate at 10 µg

    Lane 3: Mouse brain tissue lysate at 10 µg

    Lane 4: Rat brain tissue lysate at 10 µg

    Lane 5: Rat heart tissue lysate at 10 µg

    Lane 6: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 7: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa

    Observed band size: 56 kDa

    Exposure time: 3min

  • Dot Blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676), expandable thumbnail

    Dot Blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676)

    This data was developed using Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556, the same antibody clone in a different buffer formulation.Dot blot analysis of AKT1 (phospho S124) labeled with Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556 at 1/1000 dilution.
    Lane 1: phospho peptide
    Lane 2: non phospho peptide
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
    Exposure time: 3 minutes.

    Blocking and Diluting buffer buffer and concentration: 5% NFDM/TBST

  • ELISA - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676), expandable thumbnail

    ELISA - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676)

    This data was developed using Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556, the same antibody clone in a different buffer formulation.ELISA image showing specificity of Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556 to AKT1 (phospho S124) peptide only.

    Peptides concentration: 1000 ng/ml.

    Anti-AKT1 (phospho S124) antibody [EPR17680] ab183556 working dilution: 1/1700.

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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