AB250676

Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal HDAC5 phospho S498 + S498 antibody. Carrier free. Suitable for IP, ELISA, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 2 publications.

View Alternative Names

KIAA0600, HDAC5, Histone deacetylase 5, HD5, Antigen NY-CO-9

7 Images

Supplier Data

Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

This data was developed using ab183556, the same antibody clone in a different buffer formulation.

Immunoprecipitation of AKT1 from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate achieved using ab183556 at 1/100 dilution.

Lane 1 : Input : 10μg of MCF7 whole cell lysate.

Lane 2 : MCF7 whole cell lysate following IP with ab183556.

Lane 3 : negative control : IP using Rabbit monoclonal IgG (ab172730) instead of ab183556 in MCF7 whole cell lysate.

Western blot was performed using ab183556 at 1/1000 dilution.

An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] (<a href='/en-us/products/primary-antibodies/akt1-phospho-s124-antibody-epr17680-ab183556'>ab183556</a>)

Predicted band size: 47 kDa,55 kDa

Observed band size: 56 kDa

false

Supplier Data

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

This data was developed using ab183556, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (<a href='/en-us/products/primary-antibodies/akt1-phospho-s124-antibody-epr17680-ab183556'>ab183556</a>) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate, untreated at 10 µg

Lane 2:

MCF7 whole cell lysate, treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 56 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

This data was developed using ab183556, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (<a href='/en-us/products/primary-antibodies/akt1-phospho-s124-antibody-epr17680-ab183556'>ab183556</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

Mouse brain tissue lysate at 10 µg

Lane 4:

Rat brain tissue lysate at 10 µg

Lane 5:

Rat heart tissue lysate at 10 µg

Lane 6:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 7:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 56 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

This data was developed using ab183556, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (<a href='/en-us/products/primary-antibodies/akt1-phospho-s124-antibody-epr17680-ab183556'>ab183556</a>) at 1/10000 dilution

Lane 1:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate, untreated at 10 µg

Lane 2:

NIH/3T3 whole cell lysate, treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 56 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

This data was developed using ab183556, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] (<a href='/en-us/products/primary-antibodies/akt1-phospho-s124-antibody-epr17680-ab183556'>ab183556</a>) at 1/10000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate, untreated at 10 µg

Lane 2:

C6 whole cell lysate, treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

Observed band size: 56 kDa

false

Exposure time: 3min

ELISA - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

ELISA

Supplier Data

ELISA - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

This data was developed using ab183556, the same antibody clone in a different buffer formulation.

ELISA image showing specificity of ab183556 to AKT1 (phospho S124) peptide only.

Peptides concentration : 1000 ng/ml.

ab183556 working dilution : 1/1700.

Secondary antibody : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500.

Supplier Data

Dot Blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

This data was developed using ab183556, the same antibody clone in a different buffer formulation.

Dot blot analysis of AKT1 (phospho S124) labeled with ab183556 at 1/1000 dilution.
Lane 1 : phospho peptide
Lane 2 : non phospho peptide
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
Exposure time : 3 minutes.

Blocking and Diluting buffer buffer and concentration : 5% NFDM/TBST

Unconjugated

Anti-AKT1 (phospho S124) antibody [EPR17680]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17680

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ELISA, Dot, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250676 is the carrier-free version of ab183556.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKT1 also known as Protein Kinase B (PKB) is a serine/threonine kinase involved in various cellular processes. AKT1 plays an important role in mediating signals for cell survival growth and metabolism. This protein has a molecular weight of approximately 56 kDa. AKT1 is ubiquitously expressed in many tissues including the brain heart and lungs showing its importance in multiple physiological contexts. Phosphorylation of AKT1 at serine 473 denoted as p-AKT S473 is an important modification that regulates its activity.

Biological function summary

AKT1 regulates a broad spectrum of cellular functions through signaling pathways. It is an important player in the phosphatidylinositol 3-kinase (PI3K) and AKT pathway forming part of a complex involved in promoting cell survival and growth. AKT1 interacts closely with other proteins such as mTOR influencing cellular metabolism and autophagy. The phosphorylation state of AKT1 is critical for its activity with modifications like p-AKT S473 impacting its interaction with cellular substrates.

Pathways

AKT1 participates in the PI3K/AKT/mTOR signaling cascade instrumental in cell proliferation and metabolism. AKT1 integrates signals from insulin and growth factors modulating pathways that control cell growth and glucose uptake. This pathway involves proteins like mTOR and phospho-AKT which coordinate cellular responses to external stimuli. AKT1’s phosphorylation at serine 473 and the involvement of AKT1 E17K a gain-of-function mutation influence these pathways significantly.

AKT1 has associations with cancer and metabolic disorders. Dysregulation of the PI3K/AKT/mTOR pathway involving AKT1 and mTOR often results in oncogenic transformation and uncontrolled cellular proliferation. In cancer such as breast cancer AKT1 mutations including AKT1 E17K are implicated altering cell signals for growth. Additionally AKT1 is connected to metabolic disorders such as Type 2 diabetes where it affects insulin signaling and glucose metabolism highlighting its interaction with metabolic proteins like mTOR and phospho-AKT.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758).

See full target information HDAC5 phospho S498 + S498

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20230639 PubMed36820063

2023

Glycoprotein non-metastatic melanoma B interacts with epidermal growth factor receptor to regulate neural stem cell survival and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Yang,Gang Jin,Shihong Chen,Jing Luo,Wei Xu

Oncology letters 21:419 PubMed33841580

2021

Upregulation of circRNA_100395 sponges miR-142-3p to inhibit gastric cancer progression by targeting the PI3K/AKT axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyi Cheng,Guiyuan Liu,Chuanjiang Huang,Xiaojun Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free

Immunoprecipitation - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

Western blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

ELISA - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

Dot Blot - Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (AB250676)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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