Rabbit Polyclonal AKT1 phospho T342 antibody. Suitable for WB and reacts with Drosophila melanogaster samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Drosophila melanogaster Akt1 phospho T342.
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Publications
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- Cells 12:2023Neuronal Coordinates Autophagy Induction and Physiological Adaptations to Balance mTORC1 Signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAthanasios Metaxakis,Michail Pavlidis,Nektarios TavernarakisPubMed 37626835
- Cell reports 27:1979-1990.e72019Phosphatidylinositol 5 Phosphate 4-Kinase Regulates Plasma-Membrane PIP Turnover and Insulin Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanjeev Sharma,Swarna Mathre,Visvanathan Ramya,Dhananjay Shinde,Padinjat RaghuPubMed 31091438
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Drosophila melanogaster Akt1 phospho T342. The exact immunogen used to generate this antibody is proprietary information. Database link Q8INB9
Reactivity data
Select an application| WB | |
|---|---|
| Drosophila melanogaster | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info 1/500 | Notes - |
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Target data
Function
Serine/threonine kinase involved in various developmental processes (PubMed:10587646, PubMed:10962553, PubMed:11740943, PubMed:11872800, PubMed:12172554, PubMed:12893776, PubMed:14525946, PubMed:15466161, PubMed:15712201, PubMed:9601646). During early embryogenesis, acts as a survival protein (PubMed:10962553, PubMed:9601646). During mid-embryogenesis, phosphorylates and activates trh, a transcription factor required for tracheal cell fate determination (PubMed:11740943). Also regulates tracheal cell migration (PubMed:11740943, PubMed:14525946). Later in development, acts downstream of PI3K and Pk61C/PDK1 in the insulin receptor transduction pathway which regulates cell growth and organ size, by phosphorylating and antagonizing FOXO transcription factor (PubMed:10587646, PubMed:10962553, PubMed:11752451, PubMed:12893776, PubMed:24603715, PubMed:25329475, PubMed:29025897). Controls follicle cell size during oogenesis (PubMed:15712201). May also stimulate cell growth by phosphorylating Gig/Tsc2 and inactivating the Tsc complex (PubMed:12172554, PubMed:15466161). Dephosphorylation of 'Ser-586' by Phlpp triggers apoptosis and suppression of tumor growth (PubMed:10962553).
Alternative names
Akt1, CG4006, Akt, Akt1, CG4006, RAC serine/threonine-protein kinase, DAkt, DRAC-PK, Dakt1, Protein kinase B, PKB
Recommended products
Rabbit Polyclonal AKT1 phospho T342 antibody. Suitable for WB and reacts with Drosophila melanogaster samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Drosophila melanogaster Akt1 phospho T342.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Drosophila melanogaster Akt1 phospho T342. The exact immunogen used to generate this antibody is proprietary information. Database link Q8INB9
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab228808 was prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AKT1 also known as Protein Kinase B (PKB) is a serine/threonine kinase involved in various cellular processes. AKT1 plays an important role in mediating signals for cell survival growth and metabolism. This protein has a molecular weight of approximately 56 kDa. AKT1 is ubiquitously expressed in many tissues including the brain heart and lungs showing its importance in multiple physiological contexts. Phosphorylation of AKT1 at serine 473 denoted as p-AKT S473 is an important modification that regulates its activity.
- Biological function summary
AKT1 regulates a broad spectrum of cellular functions through signaling pathways. It is an important player in the phosphatidylinositol 3-kinase (PI3K) and AKT pathway forming part of a complex involved in promoting cell survival and growth. AKT1 interacts closely with other proteins such as mTOR influencing cellular metabolism and autophagy. The phosphorylation state of AKT1 is critical for its activity with modifications like p-AKT S473 impacting its interaction with cellular substrates.
- Pathways
AKT1 participates in the PI3K/AKT/mTOR signaling cascade instrumental in cell proliferation and metabolism. AKT1 integrates signals from insulin and growth factors modulating pathways that control cell growth and glucose uptake. This pathway involves proteins like mTOR and phospho-AKT which coordinate cellular responses to external stimuli. AKT1’s phosphorylation at serine 473 and the involvement of AKT1 E17K a gain-of-function mutation influence these pathways significantly.
- Associated diseases and disorders
AKT1 has associations with cancer and metabolic disorders. Dysregulation of the PI3K/AKT/mTOR pathway involving AKT1 and mTOR often results in oncogenic transformation and uncontrolled cellular proliferation. In cancer such as breast cancer AKT1 mutations including AKT1 E17K are implicated altering cell signals for growth. Additionally AKT1 is connected to metabolic disorders such as Type 2 diabetes where it affects insulin signaling and glucose metabolism highlighting its interaction with metabolic proteins like mTOR and phospho-AKT.
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Tested
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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1 product image
Western blot - Anti-AKT1 (phospho T342) antibody (ab228808)
Phosphospecificity is shown in Lane 2 where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1200 units for 30 minutes).
All lanes: Western blot - Anti-AKT1 (phospho T342) antibody (ab228808) at 1/500 dilution
Lane 1: Drosophila lysate
Lane 2: Drosophila lysate treated with lambda phosphatase (1200 units for 30 minutes).
Predicted band size: 55 kDa
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