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Rabbit Recombinant Monoclonal AKT3 phospho S473 antibody. Carrier free. Suitable for IP, Dot, WB, ICC/IF and reacts with Mouse, Synthetic peptide, Rat, Human samples. Cited in 3 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody [EPR18853] - BSA and Azide free (AB222489), expandable thumbnail
  • Immunoprecipitation - Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody [EPR18853] - BSA and Azide free (AB222489), expandable thumbnail
  • Dot Blot - Anti-AKT1 (pS473) + AKT2 (pS474) + AKT3 (pS472) antibody [EPR18853] - BSA and Azide free (AB222489), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWBICC/IF
Human
Predicted
Predicted
Expected
Predicted
Mouse
Tested
Expected
Expected
Tested
Rat
Predicted
Predicted
Expected
Predicted
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.

Targets

AKT1 phospho S473, AKT2 phospho S474

Alternative names

PKBG, AKT3, PKBG, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, RAC-PK-gamma, STK-2, PKB gamma

Recommended products

Rabbit Recombinant Monoclonal AKT3 phospho S473 antibody. Carrier free. Suitable for IP, Dot, WB, ICC/IF and reacts with Mouse, Synthetic peptide, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR18853

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab222489 is the carrier-free version of Anti-AKT1 (pS473) + AKT2 (pS474) + AKT3 (pS472) antibody [EPR18853] ab192623.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein kinase AKT also known as protein kinase B (PKB) includes three isoforms: AKT1 AKT2 and AKT3. These play a central role in cellular processes. AKT1 has a molecular weight of approximately 56 kDa AKT2 has around 55 kDa and AKT3 similarly weighs about 54 kDa. These isoforms of AKT are expressed in various tissues but AKT1 is abundantly found in most tissues AKT2 is often present in insulin-responsive tissues like skeletal muscle and adipose tissue and AKT3 is mainly in the brain. The activation of AKT involves phosphorylation which increases their kinase activity significantly. The isoforms have unique and overlapping functions offering distinct mechanical roles in cellular signaling.

Biological function summary

The AKT kinase family plays a major role in regulating vital cellular functions including metabolism cell proliferation survival and growth. It does so by catalyzing phosphorylation of a range of substrates within these pathways. AKT is often involved in complexes with other proteins to achieve these biological effects. It impacts the regulation of glycogen synthase kinase 3 (GSK3) involved in insulin response and mTOR which controls cell growth. These wide-ranging functions make AKT proteins essential in maintaining cellular homeostasis.

Pathways

AKT proteins are important components of the PI3K/AKT/mTOR signaling pathway and the insulin signaling pathway. Within these pathways they interact with and modulate activities of related proteins such as PI3K PDK1 and mTOR. This regulation helps control the balance between anabolic and catabolic processes influencing cell survival growth and metabolism. The importance of AKT kinases in these pathways marks them as key regulators of cellular responses to external and internal stimuli.

Associated diseases and disorders

AKT isoforms have strong connections to cancer and diabetes. Over-activation of AKT pathways frequently occurs in cancerous cells contributing to uncontrolled cell growth and survival. In diabetes AKT2 is particularly implicated due to its role in insulin signaling influencing how cells process glucose. Dysregulation of AKT pathways has been linked to the development and progression of these diseases where proteins like mTOR in cancer and IRS1 in diabetes show direct relation to AKT function and malfunctions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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