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Mouse Monoclonal AKT3 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AKT3 aa 100-150.

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Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human AKT3 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y243
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Predicted
Rat
Predicted
Chicken
Predicted
Cow
Predicted
Dog
Predicted

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Dog

Dilution info

-

Notes

-

Target data

Function

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.

Alternative names

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Mouse Monoclonal AKT3 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AKT3 aa 100-150.

Alternative names

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human AKT3 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y243
Clone number

66C1247.1

Purification technique

Affinity purification Protein G

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle, Store undiluted

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody [66C1247.1] (ab118509), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 antibody [66C1247.1] (ab118509)

    ab118509 at 5 µg/ml staining AKT3 in Formalin-Fixed, Paraffin-Embedded Human Brain, Cerebellum tissue, followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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